Related papers: Cell shape identification using digital holographi…
X-ray dark-field imaging creates a representation of the sample where contrast is generated by subresolution features within the volume under inspection. These are detected by a local measurement of the radiation field's angular…
We investigate the problem of 3D complex field reconstruction corresponding to unstained red blood cells (RBCs) with a single defocused off-axis digital hologram. We employ recently introduced mean gradient descent (MGD) optimization…
High-speed digital holography reveals local blood flow contrasts in the eye fundus through deterministic signal analysis, leveraging a forward scattering model of dynamically diffused light. This approach enables the estimation of absolute…
Manual operation of microscopes for repetitive tasks in cell biology is a significant bottleneck, consuming invaluable expert time, and introducing human error. Automation is essential, and while Digital Holographic Microscopy (DHM) offers…
Digital holography numerically restores three-dimensional image information using optically captured diffractive waves. The required bandwidth is larger than that of hologram pixel at a closer distance in the Fresnel diffraction regime,…
Conventionally, 3-dimensional cellular tomography can be done with light sheet or multi-angle observations. Recently, a new technique was introduced where the cell was rotated using convection currents to visualize the outer periphery (Liu…
The conversion of phase variations in an optical wavefield into intensity information is of fundamental importance for optical imaging technology including microscopy of biological cells. While conventional approaches to phase-imaging…
Epithelial cells form diverse structures from squamous spherical organoids to densely packed pseudostratified tissues. Quantification of cellular properties in these contexts requires high-resolution deep imaging and computational…
Automatic detection and segmentation of cells and nuclei in microscopy images is important for many biological applications. Recent successful learning-based approaches include per-pixel cell segmentation with subsequent pixel grouping, or…
We demonstrate wide-angle, broadband and efficient reflection holography by utilizing coupled dipole-patch nano-antenna cells to impose an arbitrary phase profile on of the reflected light. High fidelity images were projected at angles of…
A method for direct phase difference reconstruction using single-shot dual-wavelength off-axis digital holography is presented. This approach enables direct imaging of samples with high steps without the need to reconstruct phase images at…
Recent progress in phase modulation using nanofabricated electron holograms has demonstrated how the phase of an electron beam can be controlled. In this paper, we apply this concept to the correction of spherical aberration in a scanning…
Lensless in-line holography is a simple, portable, and cost-effective method of imaging especially for the biomedical microscopy applications. We propose a multiplicative gradient descent optimization based method to obtain multi-depth…
We demonstrate numerical refocusing in coherent confocal laser scanning microscopy based on synthetic optical holography. This physics-based approach implements a computational propagation on the complex signal recovered in synthetic…
We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield…
Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…
A forward model is presented to an inverse scattering problem that arises in the application of reflective Fourier ptychographic microscopy. The model allows us to determine the 3D distributions of refractive index for weakly scattering…
In this paper we apply digital in-line holography to image opaque objects through a thick plano-concave pipe. Opaque fibers and opaque particles are considered}. Analytical expression of the intensity distribution in the CCD sensor plane is…
We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…
The paper presents a hybrid bubble hologram processing approach for measuring the size and 3D distribution of bubbles over a wide range of size and shape. The proposed method consists of five major steps, including image enhancement,…