Related papers: Cell shape identification using digital holographi…
Cell shape has long been used to discern cell phenotypes and states, but the underlying premise has not been quantitatively tested. Here, we show that a single cell image can be used to discriminate its migration behavior by analyzing a…
In this paper the bright-field defocusing microscopy (DM) technique is presented. DM is able to obtain quantitative information of each plane/surface of pure phase objects, as live unlabeled cells, and its application to red blood cells…
In several areas of optics and photonics like wave propagation, digital holography, holographic microscopy, diffraction imaging, biomedical imaging and diffractive optics, the behavior of the electromagnetic waves has to be calculated with…
Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…
Many medical and biological protocols for analyzing individual biological cells involve morphological evaluation based on cell staining, designed to enhance imaging contrast and enable clinicians and biologists to differentiate between…
Conventional methods of wavefront reconstruction from the raw data of the Shack-Hartmann sensor use the focal spot shifts and discard the high-frequency information about the wavefront. Phase-retrieval-based methods treat the Hartmann…
Photometric Stereo methods seek to reconstruct the 3d shape of an object from motionless images obtained with varying illumination. Most existing methods solve a restricted problem where the physical reflectance model, such as Lambertian…
Recent advances in deep learning-based image reconstruction techniques have led to significant progress in phase retrieval using digital in-line holographic microscopy (DIHM). However, existing deep learning-based phase retrieval methods…
A method is described for the reconstruction of the amplitude and phase of the object exit wave function by phase-plate transmission electron microscopy. The proposed method can be considered as in-line holography and requires three images,…
Traditional methods for biological shape inference, such as deep learning (DL) and active contour models, face important limitations in 3D. DL approaches require large annotated datasets, which are often impractical to obtain, while active…
Laser Doppler holography (LDH) is a full-field blood flow imaging technique able to reveal human retinal and choroidal blood flow with high temporal resolution. We here report on using LDH in the anterior segment of the eye without making…
We present an original heterodyne holography method for digital holography that relies on two-dimensional heterodyne detection to record the phase and the amplitude of a f ield. The technique has been tested on objects as much as 13 mm in…
Changes in morphological characteristics of cells from two cultured cancer cell lines, HeLa and A549, induced by photodynamic treatment with Radachlorin photosensitizer have been monitored using digital holographic microscopy. The observed…
Spatial transcriptomics methods capture cellular measurements such as gene expression and cell types at specific locations in a cell, helping provide a localized picture of tissue health. Traditional visualization techniques superimpose the…
In this study, we present a detailed analysis of deflections in a butterfly wing utilizing digital holographic interferometry. Our methodology revolves around an off-axis lensless Fourier holographic setup, and we employ laser excitation to…
A single-pixel digital holography system with phase-encoded illumination using a digital micromirror device (DMD) as a spatial light modulator (SLM) is presented. The enhanced switching rate of DMDs, far exceeding the stringent frame-rate…
Ultrafast X-ray imaging provides high resolution information on individual fragile specimens such as aerosols, metastable particles, superfluid quantum systems and live biospecimen, which is inaccessible with conventional imaging…
The Smith-Purcell effect enables electromagnetic radiation across arbitrary spectral ranges by phase-matching the diffraction orders of an optical grating with the near-field of a moving electron. In this work, we introduce a novel approach…
Flow cytometry is a cornerstone technique in medical and biological research, providing crucial information about cell size and granularity through forward scatter (FSC) and side scatter (SSC) signals. Despite its widespread use, the…
We demonstrate the first use of the HoloTile Computer-Generated Holography (CGH) modality on multi-wavelength targets. Taking advantage of the sub-hologram tiling and Point Spread Function (PSF) shaping of HoloTile allows for reconstruction…