Computational refocusing in phase-resolved confocal microscopy
Abstract
We demonstrate numerical refocusing in coherent confocal laser scanning microscopy based on synthetic optical holography. This physics-based approach implements a computational propagation on the complex signal recovered in synthetic holography consistent with the wave physics and the parameters of the microscope. An experimental demonstration is shown to restore an in-focus image of a test object from data acquired at several focal plane off-sets. Numerical refocusing can provide focused views on samples with large height variation with a potential application in confocal optical surface profiling.
Cite
@article{arxiv.2411.07865,
title = {Computational refocusing in phase-resolved confocal microscopy},
author = {Martin Schnell and Melanie King and Sam Buercklin and Paulo Sarriugarte and Rainer Hillenbrand and P. Scott Carney},
journal= {arXiv preprint arXiv:2411.07865},
year = {2024}
}
Comments
5 pages, 4 figures. \c{opyright} 2023 Optica Publishing Group. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited