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An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

Super-resolution localization microscopy is based on determining the positions of individual fluorescent markers in a sample. The major challenge in reaching an ever higher localization precision lies in the limited number of collected…

Optics · Physics 2020-08-05 Daniel Böning , Franz-Ferdinand Wieser , Vahid Sandoghdar

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

The localisation of fluorophores is an important aspect of the determination of the biological function of cellular systems. Quantum correlation microscopy (QCM) is a promising technique for providing diffraction unlimited emitter…

Quantum Physics · Physics 2023-10-05 Shuo Li , Wenchao Li , Qiang Sun , Bill Moran , Timothy C. Brown , Brant C. Gibson , Andrew D. Greentree

We present a universal deep-learning method that reconstructs super-resolved images of quantum emitters from a single camera frame measurement. Trained on physics-based synthetic data spanning diverse point-spread functions, aberrations,…

Super-resolution is the problem of recovering a superposition of point sources using bandlimited measurements, which may be corrupted with noise. This signal processing problem arises in numerous imaging problems, ranging from astronomy to…

Machine Learning · Computer Science 2015-09-29 Qingqing Huang , Sham M. Kakade

We present an ultra-fast, precise, parameter-free method, which we term Deep-STORM, for obtaining super-resolution images from stochastically-blinking emitters, such as fluorescent molecules used for localization microscopy. Deep-STORM uses…

Optics · Physics 2018-05-03 Elias Nehme , Lucien E. Weiss , Tomer Michaeli , Yoav Shechtman

In single-molecule microscopy it is necessary to locate with high precision point sources from noisy observations of the spectrum of the signal at frequencies capped by $f_c$, which is just about the frequency of natural light. This paper…

Information Theory · Computer Science 2015-04-06 Veniamin I. Morgenshtern , Emmanuel J. Candes

Nanoparticles (NPs) have proven their applicability in biosensing, drug delivery, and photo-thermal therapy, but their performance depends critically on the distribution and number of functional groups on their surface. When studying…

Plasmonic nano-objects have shown great potential in enhancing biological and chemical sensing, light harvesting and energy transfer, and optical and quantum computing to name a few. Therefore, an extensive effort has been vested in…

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Resolving signals with closely spaced frequencies is central to applications in communications, spectroscopy and sensing. Recent results have shown that quantum sensing protocols can exhibit superresolution, the ability to discriminate…

Quantum Physics · Physics 2026-02-13 Joseph T. Iosue , Paraj Titum , Taohan Lin , Clare Lau , Leigh M. Norris

In fluorescence microscopy, Single Molecule Localization Microscopy (SMLM) techniques aim at localizing with high precision high density fluorescent molecules by stochastically activating and imaging small subsets of blinking emitters.…

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Daniel Freedman , Racheli Gordon , Boris Ferdman , Lucien E. Weiss , Onit Alalouf , Reut Orange , Tomer Michaeli , Yoav Shechtman

The resolution limits of classical spectroscopy can be surpassed by quantum-inspired methods leveraging the information contained in the phase of the complex electromagnetic field. Their counterpart in spatial imaging has been widely…

Optics · Physics 2024-09-02 Michał Lipka , Michał Parniak

Point-wise localization of individual fluorophores is a critical step in super-resolution microscopy and single particle tracking. Although the methods are limited by the accuracy in localizing individual flourophores, this point-wise…

Biological Physics · Physics 2017-05-08 Martin Lindén , Vladimir Ćurić , Elias Amselem , Johan Elf

Single-molecule microscopy has become an indispensable tool for biochemical analysis. The capability of characterizing distinct properties of individual molecules without averaging has provided us with a different perspective for the…

Applied Physics · Physics 2020-05-21 Miao-Hsuan Chien , Silvan Schmid

We report optical positioning single quantum dots (QDs) in planar cavity with an average position uncertainty $<$20 nm using an optimized two-color photoluminescence imaging technique. We create single-photon sources based on these QDs in…