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Paradoxically, imaging with resolution much below the wavelength $\lambda$ - now common place in the visible spectrum - remains challenging at lower frequencies, where arguably it is needed most due to the large wavelengths used. Techniques…

Optics · Physics 2023-12-12 Alessandro Tuniz , Boris T. Kuhlmey

The concept of super-resolution refers to various methods for improving the angular resolution of an optical imaging system beyond the classical diffraction limit. Although several techniques to narrow the central lobe of the illumination…

Instrumentation and Methods for Astrophysics · Physics 2017-04-11 Luca Olmi , Pietro Bolli , Luca Cresci , Francesco D'Agostino , Massimo Migliozzi , Daniela Mugnai , Enzo Natale , Renzo Nesti , Dario Panella , Lorenzo Stefani

The Extreme Ultraviolet wavelength range was one of the final windows to be opened up to astronomy. Nevertheless, it provides very important diagnostic tools for a range of astronomical objects, although the opacity of the interstellar…

Solar and Stellar Astrophysics · Physics 2015-06-17 M. A. Barstow , S. L. Casewell , J. B. Holberg , M. P. Kowalski

Super-resolution imaging is vital for optical applications, such as high capacity information transmission, real-time bio-molecular imaging and nanolithography. Technology and method of super-resolution imaging have attracted much…

Optics · Physics 2022-10-19 Yangyang Zhou , Huanyang Chen

Diffraction limits the behaviour of light in optical systems and sets the smallest achievable line width at half the wavelength. With a novel subwavelength plasmonic lens to reduce the diffraction via an asymmetry and to generate and…

Optics · Physics 2009-01-30 K. R. Chen

Over the last decade, single-molecule optical microscopy has become the gold-standard approach to decipher complex molecular processes in cellular environments. [1-3] Single-molecule fluorescence microscopy has several advantages such as…

Biological Physics · Physics 2017-11-15 Edakkattuparambil Sidharth Shibu , Nadezda Varkentina , Laurent Cognet , Brahim Lounis

Here, we report analysis and summary of research in the field of localization microscopy for optical imaging. We introduce the basic elements of super-resolved localization microscopy methods for PALM and STORM, commonly used both in vivo…

Optics · Physics 2021-02-25 Jack W Shepherd , Mark C Leake

Microscopes and various forms of interferometers have been used for decades in optical metrology of objects that are typically larger than the wavelength of light {\lambda}. However, metrology of subwavelength objects was deemed impossible…

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

Fluorescence microscopy is essential in biological and medical research, providing critical insights into cellular structures. However, limited by optical diffraction and background noise, a substantial amount of hidden information is still…

Biological Physics · Physics 2026-04-14 Xiaofeng Zhang , Yongsheng Huang , Jielong Yang , Zhili Wang , Si Chen , Linbo Liu , Xin Ge

The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

Optics · Physics 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

Microscopy is the workhorse of the physical and life sciences, producing crisp images of everything from atoms to cells well beyond the capabilities of the human eye. However, the analysis of these images is frequently little better than…

Soft Condensed Matter · Physics 2017-10-18 Matthew Bierbaum , Brian D. Leahy , Alexander A. Alemi , Itai Cohen , James P. Sethna

The application of microscopy in biomedical research has come a long way since Antonie van Leeuwenhoek discovered unicellular organisms. Countless innovations have positioned light microscopy as a cornerstone of modern biology and a method…

Optical long-baseline interferometry is a unique and powerful technique for astronomical research. Since 2004, optical interferometers have produced an increasing number of scientific papers covering various fields of astrophysics. As…

Instrumentation and Methods for Astrophysics · Physics 2018-05-28 D. Defrère , C. Aerts , M. Kishimoto , P. Léna

Optical telescopes are powerful eyes for terrestrial and astronomical detection. Here we propose a new detection method with high efficiency, strong robustness and super precision, as an enhanced technique for optical telescopes in angular…

Quantum Physics · Physics 2025-02-14 Jian Leng , Yi-Xin Shen , Zhou-Kai Cao , Xiang-Bin Wang

Advances in fluorescence microscopy have reached a plateau in improving depth-to-resolution ratios for imaging scattering tissues, highlighting an urgent need for innovative techniques. We introduce Wavelens, a groundbreaking opaque lens…

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

First proposed by Paczynski in 1986, microlensing has been instrumental in the search of compact dark matter as well as discovery and characterization of exoplanets. In this article, we provide a brief history of microlensing, especially on…

Instrumentation and Methods for Astrophysics · Physics 2017-11-16 Chien-Hsiu Lee

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

Bacterial heterogeneity is pivotal for adaptation to diverse environments, posing significant challenges in microbial diagnostics and therapeutic interventions. Recent advancements in high-resolution optical microscopy have revolutionized…

Biological Physics · Physics 2025-01-06 Dohyeon Lee , Hyun-Seung Lee , Moosung Lee , Minhee Kang , Geon Kim , Tae Yeul Kim , Nam Yong Lee , YongKeun Park
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