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For more than a century, the diffraction limit has defined the resolution achievable by passive optical imaging systems. Although some resolution improvement can be gained through classical data processing of the image, it is limited by the…

Quantum Physics · Physics 2026-05-12 A. I. Lvovsky , Michael R. Grace , Saikat Guha , Mankei Tsang , Gerardo Adesso , Nicolas Treps

As a general rule of thumb the resolution of a light microscope (i.e. the ability to discern objects) is predominantly described by the full width at half maximum (FWHM) of its point spread function (psf)---the diameter of the blurring…

Statistics Theory · Mathematics 2020-10-23 Gytis Kulaitis , Axel Munk , Frank Werner

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

Physics Education · Physics 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

The relentless pursuit of understanding matter at ever-finer scales has pushed optical microscopy to surpass the diffraction limit and produced the super-resolution microscopy which enables visualizing structures shorter than the wavelength…

Atomic time scale imaging, opening a new era for studying dynamics in microcosmos, is presently attracting immense research interesting on the global level due to its powerful ability. On the atom level, physics, chemistry, and biology are…

Optics · Physics 2023-10-19 Jingzhen Li , Yi Cai , Xuanke Zeng , Xiaowei Lu , Qifan Zhu , Yongle Zhu

Optics is an important subfield of physics required for instrument design and used in a variety of other disciplines, including materials science, physics, and life sciences such as developmental biology and cell biology. It is important to…

We demonstrate that a deep neural network can significantly improve optical microscopy, enhancing its spatial resolution over a large field-of-view and depth-of-field. After its training, the only input to this network is an image acquired…

Machine Learning · Computer Science 2017-11-21 Yair Rivenson , Zoltan Gorocs , Harun Gunaydin , Yibo Zhang , Hongda Wang , Aydogan Ozcan

Through digital imaging, microscopy has evolved from primarily being a means for visual observation of life at the micro- and nano-scale, to a quantitative tool with ever-increasing resolution and throughput. Artificial intelligence, deep…

This paper is a review of recent mathematical and computational advances in optical tomography. We discuss the physical foundations of forward models for light propagation on microscopic, mesoscopic and macroscopic scales. We also consider…

Analysis of PDEs · Mathematics 2009-07-16 Simon Arridge , John Schotland

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

We discuss recently emerging applications of the state-of-art deep learning methods on optical microscopy and microscopic image reconstruction, which enable new transformations among different modes and modalities of microscopic imaging,…

Machine Learning · Computer Science 2018-07-23 Yair Rivenson , Aydogan Ozcan

Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record…

Optics · Physics 2021-08-26 Zengbo Wang

Optical spectroscopy constitutes the historical path to accumulate basic knowledge on the atom and its structure. Former work based on fluorescence and resonance ionization spectroscopy enabled identifying optical spectral lines up to…

Atomic Physics · Physics 2020-07-14 Mustapha Laatiaoui , Alexei A. Buchachenko , Larry A. Viehland

The optical resolution of a digital camera is one of its most crucial parameters with broad relevance for consumer electronics, surveillance systems, remote sensing, or medical imaging. However, resolution is physically limited by the…

Computer Vision and Pattern Recognition · Computer Science 2018-12-27 Thomas Köhler

Adaptive optics (AO) is a powerful image correction technique with proven benefits for many life-science microscopy methods. However, the complexity of adding a reflective wavefront modulator and a wavefront sensor into already complicated…

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…

The year 2015 was the International Year of Light. It marked, however, also the 20th anniversary of the first observation of Bose-Einstein condensation in atomic vapors by Eric Cornell, Carl Wieman and Wolfgang Ketterle. This discovery can…

Quantum Gases · Physics 2017-01-04 Alessio Celi , Anna Sanpera , Veronica Ahufinger , Maciej Lewenstein

A thorough understanding of biological species and of emerging nanomaterials requires, among others, their in-depth characterization with optical techniques capable of nano-resolution. Nanoscopy techniques based on tip-enhanced optical…

An optical microscope enables image-based findings and diagnosis on microscopic targets, which is indispensable in many scientific, industrial and medical settings. Majority of microscope users are accustomed to standard benchtop…

Optics · Physics 2025-05-06 Keiichiro Toda , Miu Tamamitsu , Ryoichi Horisaki , Takuro Ideguchi

I propose a superoscillation measurement method for subdiffraction incoherent optical sources, with potential applications in astronomy, remote sensing, fluorescence microscopy, and spectroscopy. The proposal, based on coherent optical…

Quantum Physics · Physics 2022-10-10 Mankei Tsang