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Improvement of label-free far-field resolution of optical imaging is possible with prior knowledge of the object such as its sparsity or accumulated by a posteriori examination of a similar class of object1-4. We show that the sole…

Optics · Physics 2026-02-03 Taeyong Chang , Giorgio Adamo , Nikolay I. Zheludev

As a revolutionary observation tool in life science, biomedical, and material science, optical microscopy allows imaging of samples with high spatial resolution and a wide field of view. However, conventional microscopy methods are limited…

Optics · Physics 2023-03-10 Yichang Shou , Jiawei Liu , Hailu Luo

Segmentation, or the outlining of objects within images, is a critical step in the measurement and analysis of cells within microscopy images. While improvements continue to be made in tools that rely on classical methods for segmentation,…

Quantitative Methods · Quantitative Biology 2024-03-15 Nodar Gogoberidze , Beth A. Cimini

Modern imaging technologies are widely based on classical principles of light or electromagnetic wave propagation. They can be remarkably sophisticated, with recent successes ranging from single molecule microscopy to imaging far-distant…

Electrochemistry exploits local current heterogeneities at various scales ranging from the micrometer to the nanometer. The last decade has witnessed unprecedented progress in the development of a wide range of electroanalytical techniques…

Optics · Physics 2022-11-16 Frederic Kanoufi

Optical detection of nanoscale objects without relying on fluorescence is a current challenge due to their extremely weak interaction with light. Resonator-enhanced absorption microscopy is a novel tool to heavily boost the light-matter…

Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy (SMLM), have pushed…

Standard pedagogy introduces optics as though it were a consequence of Maxwell's equations, and only grudgingly admits, usually in a rushed aside, that light has a particulate character that can somehow be reconciled with the wave picture.…

Other Quantitative Biology · Quantitative Biology 2018-09-20 Philip C. Nelson

Nonlinear optical microscopy provides elegant means for label-free imaging of biological samples and condensed matter systems. The widespread areas of application could even be increased if resolution was improved, which is currently…

Perfect lensing using negative refractive index materials and radiationless electromagnetic interference both provide extreme subwavelength focusing by "amplifying" evanescent wave components that are usually lost. This paper provides a…

Optics · Physics 2015-06-03 Reuven Gordon

Expansion Microscopy is a super-resolution technique in which physically enlarging samples in an isotropic manner increases inter-molecular distances such that nano-scale structures can be resolved using light microscopy. This is…

Neurons and Cognition · Quantitative Biology 2025-01-22 Shakila Behzadi , Jacquelin Ho , Zainab Tanvir , Gal Haspel , Limor Freifeld , Kristen E. Severi

The resolution of far-field optical microscopes, which rely on propagating optical modes, is widely believed to be limited because of diffraction to a value on the order of a half-wavelength $\lambda /2$ of the light used. Although…

Materials Science · Physics 2009-11-10 Igor I. Smolyaninov , Christopher C. Davis , Jill Elliott , Anatoly V. Zayats

Convex optimization recently emerges as a compelling framework for performing super resolution, garnering significant attention from multiple communities spanning signal processing, applied mathematics, and optimization. This article offers…

Signal Processing · Electrical Eng. & Systems 2020-04-22 Yuejie Chi , Maxime Ferreira Da Costa

A microscope based on the Linnik interferometer was designed, built, and tested. Two methods were used for interference pattern measurement: phase-shifting and polarized single-shot methods. The former uses a low coherence light emitting…

Optics · Physics 2024-06-25 Sergei V. Anishchik , Marcos Dantus

One of the fundamental limits of classical optical microscopy is the diffraction limit of optical resolution. It results from the finite bandwidth of the optical transfer function (or OTF) of an optical microscope, which restricts the…

Optics · Physics 2022-03-11 Sjoerd Stallinga , Niels Radmacher , Antoine Delon , Jörg Enderlein

Optical microscopy has so far been restricted to superficial layers, leaving many important biological questions unanswered. Random scattering causes the ballistic focus, which is conventionally used for image formation, to decay…

Optics · Physics 2012-08-28 Ke Si , Reto Fiolka , Meng Cui

{\it ' Seeing is believing '} \cite{north06}- is the fundamental philosophy of optical imaging based on light microscopy \cite{ntziachristos10}. Since the pioneering works of Antonie van Leeuwenhoek and Robert Hooke in the 17th century,…

Biological Physics · Physics 2020-12-29 Swayamshree Patra , Swagata Dey , Krishanu Ray , Debashish Chowdhury

Although far-IR -- mm-wave astronomy has now been developing for several decades, access to this portion of the spectrum remains difficult and limited. Relative to their optical-wavelength counterparts, the submillimeter observatories are…

Astrophysics · Physics 2017-01-18 C. Matt Bradford , Jonas Zmuidzinas

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…