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Precisely measuring the three-dimensional position and orientation of individual fluorophores is challenging due to the substantial photon shot noise in single-molecule experiments. Facing this limited photon budget, numerous techniques…

Optics · Physics 2021-02-02 Oumeng Zhang , Matthew D. Lew

We propose and demonstrate, through simulation and experiment, how the interaction of an optical field within a waveguide designed for chemical sensing and, more generally, evanescent field spectroscopy can be enhanced substantially by…

Accurately tracking particles and determining their coordinate along the optical axis is a major challenge in optical microscopy, especially when extremely high precision is needed. In this study, we introduce a deep learning approach using…

Image and Video Processing · Electrical Eng. & Systems 2025-08-05 Andrey Alexandrov , Giovanni Acampora , Giovanni De Lellis , Antonia Di Crescenzo , Chiara Errico , Daria Morozova , Valeri Tioukov , Autilia Vittiello

Modern optical nano-elements pursue ever-smaller sizes and individualized functionalities. Those elements that can efficiently manipulate the magnetic field of light boast promising future applications with a great challenge: the magnetic…

All-dielectric optical nano-resonators, exhibiting exotic near-field distributions upon excitations, have emerged as low-loss, versatile and highly adaptable components in nanophotonic structures for manipulating electromagnetic waves and…

We introduce a full field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering.…

The near-field scanning optical microscopic (NSOM) imaging of Au nanoparticles with size in the sub-wavelength limit (<wavelength/2N.A.) is reported. The NSOM imaging technique can resolve the objects which is beyond the scope of optical…

Materials Science · Physics 2017-05-23 Prajit Dhara , A. K. Sivadasan

We introduce optical polarization-insensitive high pass filters based on total internal reflection of light at the interface of two dielectric media (1D) and Bragg reflection of a multilayer stack (2D) in transmission mode. The wavevectors…

Optics · Physics 2019-11-26 Ali Pour Mohammad Qoli Vafa , Parisa Karimi , Amin Khavasi

Optical detection of nanoscale objects without relying on fluorescence is a current challenge due to their extremely weak interaction with light. Resonator-enhanced absorption microscopy is a novel tool to heavily boost the light-matter…

Single-molecule microscopy has become an indispensable tool for biochemical analysis. The capability of characterizing distinct properties of individual molecules without averaging has provided us with a different perspective for the…

Applied Physics · Physics 2020-05-21 Miao-Hsuan Chien , Silvan Schmid

The recent development of electron sensitive and pixelated detectors has attracted the use of four-dimensional scanning transmission electron microscopy (4D-STEM). Here, we present a precession electron diffraction assisted 4D-STEM…

Instrumentation and Detectors · Physics 2021-10-04 Jiwon Jeong , Niels Cautaerts , Gerhard Dehm , Christian H. Liebscher

To overcome the limit of diffraction while achieving the superresolution technique, solid immersion lenses are the key optical elements for data storage and nanophotonics applications. Recent demonstrations have shown how different…

Optics · Physics 2026-02-24 Subhankar Roy , Jianping Hu , M Ummal Momeen

We present a new kind of fluorescent oxide nanoparticles with properties well suited to active-tip based near-field optics. These particles with an average diameter in the range 5-10 nm are produced by Low Energy Cluster Beam Deposition…

Materials Science · Physics 2008-12-09 A. Cuche , B. Masenelli , G. Ledoux , D. Amans , C. Dujardin , Y. Sonnefraud , P. Melinon , S. Huant

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

Imaging of the optical properties of individual nanosystems beyond fluorescence can provide a wealth of information. However, the minute signals for absorption and dispersion are challenging to observe, and only specialized techniques…

Optics · Physics 2015-09-10 Matthias Mader , Jakob Reichel , Theodor W. Hänsch , David Hunger

Tracking cells in 3D at high speed continues to attract extensive attention for many biomedical applications, such as monitoring immune cell migration and observing tumor metastasis in flowing blood vessels. Here, we propose a deep…

Optics · Physics 2018-05-16 Kan Liu , Hui Qiao , Jiamin Wu , Haoqian Wang , Lu Fang , Qionghai Dai

Optical nanofibers (ONF) of subwavelength dimensions confine light in modes with a strong evanescent field that can trap, probe, and manipulate nearby quantum systems. To measure the evanescent field and propagating modes, and to optimize…

In single molecule localisation super-resolution microscopy the need for repeated image capture limits the imaging speed, while the size of fluorescence probes limits the possible theoretical localisation resolution. Here, we demonstrated a…

Single molecule localization microscopy has the potential to resolve structural details of biological samples at the nanometer length scale. However, to fully exploit the resolution it is crucial to account for the anisotropic emission…