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Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…
This paper introduces Spectral Incoherent Diffractive Imaging (SIDI) as a novel method for achieving dark-field imaging of nanostructures with heterogeneous oxidation states. With SIDI, shifts in photoemission profiles can be spatially…
Interferometric scattering (iSCAT) microscopy is an emerging label-free technique optimized for the sensitive detection of nano-matter. Previous iSCAT studies have approximated the point spread function in iSCAT by a Gaussian intensity…
Optical imaging below the limit of light diffraction offers an unprecedented opportunity to study outlook, organization, interactions or in-situ functioning of sub-micrometer, highly transparent objects such as subcellular structures in…
Scattering-type scanning near-field optical microscopy (s-SNOM) is instrumental in exploring polaritonic behaviors of two-dimensional (2D) materials at the nanoscale. A sharp s-SNOM tip couples momenta into 2D materials through phase…
We recently (Optics Express 17, 19969 (2009)) introduced an all-optical method for grafting onto the apex of an optical tip a single 20 nm nanodiamond with single color-center occupancy and used the resulting single-photon tip in scanning…
A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…
Super-resolution microscopy is crucial for imaging sub-wavelength biological structures. However, most techniques rely on nonlinear saturation or stochastic switching of emitters, limiting imaging speed and increasing phototoxicity. Here,…
Automation in optical microscopy is critical for enabling high-throughput imaging across a wide range of biomedical applications. Among the essential components of automated systems, robust autofocusing plays a pivotal role in maintaining…
We demonstrate a photon-sensitive, three-dimensional camera by active near-infrared illumination and fast time-of-flight gating. It uses pico-second pump pulses to selectively up-convert the backscattered photons according to their…
Molecular dipoles near interfaces emit highly directional radiation due to near-field interactions, making surface-bound fluorophores sensitive probes of local physicochemical changes. We introduce smart coverslips, stably coated with…
Nanoscale strain control of exciton funneling is an increasingly critical tool for the scalable production of single photon emitters (SPEs) in two-dimensional materials. However, conventional far-field optical microscopies remain…
The association of scanning transmission electron microscopy (STEM) and the detection of a diffraction pattern at each probe position (so-called 4D-STEM) represents one of the most promising approaches to analyze structural properties of…
An improved method of remote optical absorption spectroscopy and hyperspectral optical absorption imaging is described which takes advantage of the photoacoustic remote sensing detection architecture. A wide range of photoacoustic…
Recent advances in Nanotechnologies have prompted the need for tools to accurately and non-invasively manipulate individual nano-objects. Among possible strategies, optical forces have been foreseen to provide researchers with nano-optical…
Effective light extraction from optically active solid-state spin centres inside high-index semiconductor host crystals is an important factor in integrating these pseudo-atomic centres in wider quantum systems. Here we report increased…
We consider the excitation of electrons in semiconducting carbon nanotubes by photons from the evanescent field created by a subwavelength-diameter optical fiber. The strongly changing evanescent field of such nanofibers requires dropping…
We characterize nano-textured surfaces by optical diffraction techniques using an adapted commercial light microscope with two detectors, a CCD camera and a spectrometer. The acquisition and analyzing time for the topological parameters…
Porous nanosponges, percolated with a three-dimensional network of 10-nm sized ligaments, recently emerged as promising substrates for plasmon-enhanced spectroscopy and (photo-)catalysis. Experimental and theoretical work suggests surface…
This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…