Related papers: Correction of cell-induced optical aberrations in …
We report on the recently emerging (Laser) Light Sheet based Fluorescence Microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects non-destructively in high-resolution through virtual…
The spatio-temporal organization of proteins within the cell membrane can affect numerous biological functions, including cell signaling, communication, and transportation. Deviations from normal spatial arrangements have been observed in…
Dynamic full-field optical coherence microscopy (d-FF-OCM) is a label-free imaging technique that captures intrinsic subcellular motions to generate functional contrast. This dynamic approach yields images with fluorescence-like contrast,…
Control of light through a microscope objective with a high numerical aperture is a common requirement in applications such as optogenetics, adaptive optics, or laser processing. Light propagation, including polarization effects, can be…
We present a novel learning-based method to build a differentiable computational model of a real fluorescence microscope. Our model can be used to calibrate a real optical setup directly from data samples and to engineer point spread…
Accelerating imaging speed in optical microscopy is often realized at the expense of image contrast, image resolution, and detection sensitivity- a common predicament for advancing high-speed and high-throughput cellular imaging. We here…
We present a scheme for active compensation of complex extrinsic polarization perturbations introduced into an optical system. Imaging polarimeter is used to measure the polarization state across a beam profile and a liquid crystal spatial…
Multicolor super-resolution imaging remains an intractable challenge for both far-field and near-field based super-resolution techniques. Planar super-oscillatory lens (SOL), a far-field subwavelength-focusing diffractive lens device, holds…
Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Modeling these features is ever more important in quantitatively interpreting microscopy images collected at scales on par or…
We investigated the nanometer scale height fluctuations of 3T3 fibroblast cells with the atomic force microscope (AFM) under physiological conditions. Correlation between these fluctuations and lateral cellular motility can be observed.…
Orbital angular momentum (OAM) of photons is carried upon the wave front of an optical vortex and is important in physics research due to its fundamental degree of freedom. As for the interaction with materials, the optical OAM was shown to…
Strong turbulence conditions create amplitude aberrations through the effects of near-field diffraction. When integrated over long optical path lengths, amplitude aberrations (seen as scintillation) can nullify local areas in the recorded…
Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…
We describe an adaptive optics technique for two-photon microscopy in which the deformable mirror used for aberration compensation is positioned in a plane conjugate to the plane of the aberration. We demonstrate in a proof-of-principle…
Since 2014, the LBT's First Light Adaptive Optics (FLAO) system has also included a seldom used capability, known as Enhanced Seeing Mode (ESM), that can improve the angular resolution over a 4' x 4' field of view (FOV). In full AO…
By employing a series of massively parallel ab-initio simulations, we study how optical forces act on biological matter subject to morphological disease. As a representative case study, we here consider the case of Plasmodium Falciparum on…
Optical photothermal microscopy is a powerful, emerging method that overcomes the diffraction limit in infrared hyperspectral imaging by utilizing a visible probe laser beam to detect local temperature-induced modulation at the visible…
Point-wise localization of individual fluorophores is a critical step in super-resolution microscopy and single particle tracking. Although the methods are limited by the accuracy in localizing individual flourophores, this point-wise…
Adaptive optics (AO) systems have significantly improved astronomical imaging capabilities over the last decade, and are revolutionizing the kinds of science possible with 4-5m class ground-based telescopes. A thorough understanding of AO…
Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in…