Related papers: Correction of cell-induced optical aberrations in …
There is widespread interest in estimating the fluorescence properties of natural materials in an image. However, the separation between reflected and fluoresced components is difficult, because it is impossible to distinguish reflected and…
Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…
A magnetometric technique is demonstrated that may be suitable for precision measurements of fields ranging from the sub-microgauss level to above the Earth field. It is based on resonant nonlinear magneto-optical rotation caused by atoms…
Fourier Ptychographic Microscopy (FPM) is a computational technique that achieves a large space-bandwidth product imaging. It addresses the challenge of balancing a large field of view and high resolution by fusing information from multiple…
In the past years, optical fluorescence microscopy (OFM) made steady progress towards increasing the localisation precision of fluorescent emitters in biological samples. The high precision achieved by these techniques has prompted new…
Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane was introduced to measure diffusion and interactions of fluorescent components in free standing biomembranes. Using a confocal laser…
Fourier ptychographic microscopy (FPM) is a powerful computational imaging modality that achieves high space-bandwidth product imaging for biomedical samples. However, its adoption is limited by slow data acquisition due to the need for…
Oblique plane microscopy is a method enabling light-sheet fluorescence imaging through a single microscope objective lens by focusing on a tilted plane within the sample. To focus the fluorescence emitted by the oblique plane on a camera,…
Studying the internal structure of complex samples with light is an important task, but a difficult challenge due to light scattering. While the complex optical distortions induced by multiple scattering can be effectively undone with the…
We show how the effects of azimuthal optical aberrations on singular light beams can result in an intensity modulation in the beam waist or focal point spread function (PSF) that is directly proportional to the amplitude of the applied…
We demonstrate an optical frequency analysis method using the Fourier transform of detection times of fluorescence photons emitted from a single trapped 40Ca+ ion. The response of the detected photon rate to the relative laser frequency…
Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) to segment the Fourier Plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional(3D)…
Traditional full-field optical coherence tomography (FF-OCT) is effective for rapid cross-sectional imaging but often suffers from incoherent signals due to imbalanced light intensities between the sample and reference arms. While the…
The plasma membrane of living cells is compartmentalized at multiple spatial scales ranging from the nano- to the meso-scale. This non-random organization is crucial for a large number of cellular functions. At the nanoscale, cell membranes…
The main objective of the present project is to explore the viability of an adaptive optics control system based exclusively on Field Programmable Gate Arrays (FPGAs), making strong use of their parallel processing capability. In an…
High-resolution tissue imaging is often compromised by sample-induced optical aberrations that degrade resolution and contrast. While wavefront sensor-based adaptive optics (AO) can measure these aberrations, such hardware solutions are…
Optical fluorescence imaging is capable of measuring both the spatial and rotational dynamics of single molecules. However, unavoidable measurement noise will result in inaccurate estimates of rotational dynamics, causing a molecule to…
In optical metrological protocols to measure physical quantities, it is, in principle, always beneficial to increase photon number to improve measurement precision. However, practical constraints prevent arbitrary increase of n due to the…
Multiconjugate adaptive optics (MCAO) systems have the potential to deliver diffraction-limited images over much larger fields of view than traditional single conjugate adaptive optics systems. In MCAO, the high altitude deformable mirrors…
In this article, we report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field,…