Related papers: Correction of cell-induced optical aberrations in …
Fluorescence Correlation Spectroscopy (FCS) is widely used to detect and quantify diffusion processes at the molecular level. The molecules of which diffusion is studied are marked with fluorescent dyes. It is commonly maintained that this…
We report experimental evidence and correction of defocus in full-field OCT of biological samples due to mismatch of the refractive index of biological tissues and water. Via a metric based on the image quality, we demonstrate that we are…
Optical interferometers increasingly use single-mode fibers as spatial filters to convert varying wavefront distortion into intensity fluctuations which can be monitored for accurate calibration of fringe amplitudes. Here I propose using an…
Patient-specific therapies require that cells be manufactured in multiple batches of small volumes, making it a challenge for conventional modes of quality control. The added complexity of inherent variability (even within batches)…
Normal thermal fluctuations of the cell membrane have been studied extensively using high resolution microscopy and focused light, particularly at the peripheral regions of a cell. We use a single probe particle attached non-specifically to…
We introduce a full field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering.…
Adaptive optics (AO) are reconfigurable devices that compensate for wavefront distortions or aberrations in optical systems such as microscopes, telescopes and ophthalmoscopes. Aberrations have detrimental effects that can reduce imaging…
Fourier microscopy is becoming an increasingly important tool for the analysis of optical nanostructures and quantum emitters. However, achieving quantitative Fourier space measurements requires a thorough understanding of the impact of…
Numerical simulation of Fresnel diffraction with fast Fourier transform (FFT) is widely used in optics, especially computer holography. Fresnel diffraction with FFT cannot set different sampling rates between source and destination planes,…
We describe a new concept to correct for scintillation noise on high-precision photometry in large and extremely large telescopes using telemetry data from adaptive optics (AO) systems. Most wide-field AO systems designed for the current…
Optical interference is not only a fundamental phenomenon that has enabled new theories of light to be derived but it has also been used in interferometry for the measurement of small displacements, refractive index changes and surface…
Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…
Many adaptive optics systems operate by measuring the distortion of the wavefront in one wavelength range and performing the scientific observations in a second, different wavelength range. One common technique is to measure wavefront…
Fluorescence microscopy (FM) imaging is a fundamental technique for observing live cell division, one of the most essential processes in the cycle of life and death. Observing 3D live cells requires scanning through the cell volume while…
Diffraction-limited imaging in epi-fluorescence microscopy remains a challenge when sample aberrations are present or when the region of interest rests deep within an inhomogeneous medium. Adaptive optics is an attractive solution albeit…
Alignment-based optically pumped magnetometers (OPMs) are capable of measuring oscillating magnetic fields with high sensitivity in the fT/sqrt(Hz) range. Until now, alignment-based magnetometers have only used paraffin-coated vapour cells…
A Deformable Mirror (DM) is an important component of an Adaptive Optics system. It is known that an on-axis spherical/parabolic optical component, placed at an angle to the incident beam introduces defocus as well as astigmatism in the…
Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…
Accurate optical sensing and micromanipulation requires sensitive measurements of the position, orientation, and dynamics of small particles--and sometimes even large objects--under consideration. The signals acquired in the process,…
Structured light, where complex optical fields are tailored in all their degrees of freedom, has become highly topical of late, advanced by a sophisticated toolkit comprising both linear and nonlinear optics. Removing undesired structure…