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Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Daniel Freedman , Racheli Gordon , Boris Ferdman , Lucien E. Weiss , Onit Alalouf , Reut Orange , Tomer Michaeli , Yoav Shechtman

Super-resolution imaging using sub-diffraction field localization by micron sized transparent beads (microspheres) was recently demonstrated [1]. Practical applications in microscopy require control over the positioning of the microspheres.…

Optics · Physics 2013-12-25 Leonid A. Krivitsky , Jia Jun Wang , Zengbo Wang , Boris Lukiyanchuk

Super-resolution mapping of the 3D orientation of fluorophores reveals the alignment of biological structures where the fluorophores are tightly attached, and thus plays a vital role in studying the organization and dynamics of…

Optics · Physics 2024-01-23 Xiaowei Liu , Mingwei Tang , Ning Zhou , Chenlei Pang , Zhong Wen , Xu Liu , Qing Yang

Optical beams and starlight distorted by atmospheric turbulence can be corrected with adaptive optics systems to enable efficient coupling into single-mode fibers. Deformable mirrors, used to flatten the wavefront in astronomical…

Here, we report analysis and summary of research in the field of localization microscopy for optical imaging. We introduce the basic elements of super-resolved localization microscopy methods for PALM and STORM, commonly used both in vivo…

Optics · Physics 2021-02-25 Jack W Shepherd , Mark C Leake

We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based $\ell_0$…

Medical Physics · Physics 2022-07-12 Vasiliki Stergiopoulou , Luca Calatroni , Sébastien Schaub , Laure Blanc-Féraud

Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function because of its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by…

Biological Physics · Physics 2020-01-08 Courtney Johnson , Jack Exell , Jonathon Kuo , Kevin Welsher

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

An optical imager that exploits off-center image rotation to encode both the lateral and depth coordinates of point sources in a single snapshot can perform 3D localization and tracking of space debris. When actively illuminated, unresolved…

Instrumentation and Methods for Astrophysics · Physics 2018-12-24 Chao Wang , Robert Plemmons , Sudhakar Prasad , Raymond Chan , Mila Nikolova

Diffusion models have recently emerged as a powerful technique in image generation, especially for image super-resolution tasks. While 2D diffusion models significantly enhance the resolution of individual images, existing diffusion-based…

Computer Vision and Pattern Recognition · Computer Science 2024-11-27 Bohao Chen , Yanchao Zhang , Yanan Lv , Hua Han , Xi Chen

Speckle photography can be used to monitor deformations of solid surfaces. The measuring characteristics, such as range or lateral resolution depend heavily on the optical recording and illumination set-up. This paper shows how, by the…

Optics · Physics 2014-01-30 Jose M. Diazdelacruz

We present a superpixel method for full spatial phase and amplitude control of a light beam using a digital micromirror device (DMD) combined with a spatial filter. We combine square regions of nearby micromirrors into superpixels by low…

Optics · Physics 2015-06-19 Sebastianus A. Goorden , Jacopo Bertolotti , Allard P. Mosk

We introduce "microdeflectometry", a novel technique for measuring the microtopography of specular surfaces. The primary data is the local slope of the surface under test. Measuring the slope instead of the height implies high information…

Optics · Physics 2009-11-13 Gerd Häusler , Claus Richter , Karl-Heinz Leitz , Markus C. Knauer

Deconvolution is the most widely used aberration correction technique in microscopy, however most techniques assume that the aberrations are the same for each point in the image, which is rarely true. Methods for tracking spatially varying…

Optics · Physics 2025-12-02 Jakub Czuchnowski , Chuan Li , Hongli Ni , Brandon Weissbourd , Jerome Mertz

Improving axial resolution is of paramount importance for three-dimensional optical imaging systems. Here, we investigate the ultimate precision in axial localization using vortex beams. For Laguerre-Gauss beams, this limit can be achieved…

Quantum Physics · Physics 2021-03-09 D. Koutny , Z. Hradil , J. Rehacek , L. L. Sanchez-Soto

We present the development of extended diffraction tomography, a new approach to the solution of the linear seismic waveform inversion problem. This method has several appealing features, such as the use of arbitrary depth-dependent…

Geophysics · Physics 2009-05-05 R. B. Schlottmann

A flexible multimode fiber is an exceptionally efficient tool for in vivo deep tissue imaging. Recent advances in compressive multimode fiber sensing allow for imaging with sub-diffraction spatial resolution and sub-Nyquist speed. At…

Optics · Physics 2024-07-17 Zhouping Lyu , Shih-Te Hung , Carlas S. Smith , Lyubov V. Amitonova

Astronomical telescopes suffer from a tradeoff between field of view (FoV) and image resolution: increasing the FoV leads to an optical field that is under-sampled by the science camera. This work presents a novel computational imaging…

Instrumentation and Methods for Astrophysics · Physics 2025-08-07 Robin Swanson , Esther Y. H. Lin , Masen Lamb , Suresh Sivanandam , Kiriakos N. Kutulakos

The far-field resolution of optical imaging systems is restricted by the Abbe diffraction limit, a direct result of the wave nature of light. One successful technological approach to circumventing this limit is to reduce the effective size…

We present a novel automatic adaptive aperture photometry algorithm for measuring the total magnitudes of merging galaxies with irregular shapes. First, we use a morphological pattern recognition routine for identifying the shape of an…

Instrumentation and Methods for Astrophysics · Physics 2017-02-08 Jen-Chao Huang , Chorng-Yuan Hwang
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