Related papers: Improved 3D Superresolution Localization Microscop…
Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…
Adaptive optics can focus light through opaque media by compensating the random phase delay acquired while crossing a scattering curtain. The technique is commonly exploited in many fields, including astrophysics, microscopy, biomedicine…
Methods of three-dimensional deconvolution with a point-spread function as frequently employed in optical microscopy to reconstruct true three-dimensional distribution of objects are extended to holographic reconstructions. Two such schemes…
One of the most common methods for reconstructing three-dimensional (3D) images of real objects is digital holography. This technique relies on the use of electro-optical devices that modify the phase or amplitude of light fields in a…
Microscope objectives achieve near diffraction-limited performance only when used under the conditions they are designed for. In non-standard geometries, such as thick cover slips or curved surfaces, severe aberrations arise, inevitably…
Optical phase-space functions describe spatial and angular information simultaneously; examples include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration…
The purpose of this study is to enable in-vivo three-dimensional (3-D) ultrasound localization microscopy (ULM) of posterior ocular microvasculature using a 256-channel system and a 1024-element matrix array, and to overcome limitations of…
To overcome the half a wavelength resolution limitations of ultrasound imaging, microbubbles (MBs) have been utilized widely in the field. Conventional MB localization methods are limited whether by exhaustive parameter tuning or…
Adaptive optics in combination with multi-photon techniques is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators which are reflective, diffractive, or…
This letter presents a theoretical and experimental study on the viability of obtaining three dimensional super-resolution (i.e. resolution overcoming the diffraction limit for all directions in space) by means of metamaterial slab lenses.…
Super-resolution ultrasound (SRUS) imaging through localising and tracking sparse microbubbles has been shown to reveal microvascular structure and flow beyond the wave diffraction limit. Most SRUS studies use standard delay and sum (DAS)…
Three-photon (3P) microscopy is getting traction due to its superior performance in deep tissues. Yet, aberrations and light scattering still pose one of the main limitations in the attainable depth ranges for high-resolution imaging. Here,…
Control of light through a microscope objective with a high numerical aperture is a common requirement in applications such as optogenetics, adaptive optics, or laser processing. Light propagation, including polarization effects, can be…
Adaptive optics (AO) offers an opportunity to stabilize an image and maximize the spatial resolution achievable by ground based telescopes by removing the distortions due to the atmosphere. Typically, the deformable mirror in an AO system…
The most ubiquitous form of computational aberration correction for microscopy is deconvolution. However, deconvolution relies on the assumption that the point spread function is the same across the entire field-of-view. This assumption is…
Structured illumination microscopy (SIM) is an optical super-resolution technique that enables live-cell imaging beyond the diffraction limit. Reconstruction of SIM data is prone to artefacts, which becomes problematic when imaging highly…
Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in the field of biological microscopy. To address this challenge, we report an augmented version of light field microscopy,…
In the ELTs era, where the need for versatile and innovative solutions to produce very high spatial resolution images has become a major issue, the search of synergies with other science fields seems a logic step. One of the considered…
We present a promising approach to the extremely fast sensing and correction of small wavefront errors in adaptive optics systems. As our algorithm's computational complexity is roughly proportional to the number of actuators, it is…
Illuminating or imaging samples from a broad angular range is essential in a wide variety of computational 3D imaging and resolution-enhancement techniques, such as optical projection tomography (OPT), optical diffraction tomography (ODT),…