English
Related papers

Related papers: Confocal supercritical angle fluorescence microsco…

200 papers

3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…

Optics · Physics 2024-03-01 Pawel Szczypkowski , Monika Pawlowska , Radek Lapkiewicz

The diffraction limit is a fundamental barrier in optical microscopy, which restricts the smallest resolvable feature size of a microscopic system. Microsphere-based microscopy has proven to be a promosing tool for challenging the…

The resolution of far-field optical microscopes, which rely on propagating optical modes, is widely believed to be limited because of diffraction to a value on the order of a half-wavelength $\lambda /2$ of the light used. Although…

Materials Science · Physics 2009-11-10 Igor I. Smolyaninov , Christopher C. Davis , Jill Elliott , Anatoly V. Zayats

High-contrast imaging provided by a coronagraph is critical for the direction imaging of the Earth-like planet orbiting its bright parent star. A major limitation for such direct imaging is the speckle noise that is induced from the…

Instrumentation and Methods for Astrophysics · Physics 2015-10-14 JiangPei Dou , DeQing Ren , YongTian Zhu , Xi Zhang

Spectroscopic photoemission microscopy is a well-established method to investigate the electronic structure of surfaces. In modern photoemission microscopes the electron optics allows imaging of the image plane, momentum plane, or…

An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…

Optics · Physics 2009-11-11 C. Vinegoni , T. Ralston , W. Tan , W. Luo , D. L. Marks , S. A. Boppart

Surface molecules, distributed in diverse patterns and clusters on cell membranes, influence vital functions of living cells. It is therefore important to understand their molecular surface organisation under different physiological and…

We demonstrate a Fourier Imaging system with centimeter field of view(FOV), and 0.0042{\deg}angular resolution at C band, for the entire 180{\deg} radiation angular range. To pursue omnidirectional Fourier imaging without aberration, a…

Optics · Physics 2018-09-07 Jinxi Huang

The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demonstrated using three-dimensional quantitative phase imaging methods,…

Optical coherence tomography (OCT) microscope (OCM) uses a high-numerical-aperture objective to achieve cellular-level lateral resolution. However, its practical imaging depth range is limited by the depth of focus (DOF). Although…

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

Physics Education · Physics 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

I propose a superoscillation measurement method for subdiffraction incoherent optical sources, with potential applications in astronomy, remote sensing, fluorescence microscopy, and spectroscopy. The proposal, based on coherent optical…

Quantum Physics · Physics 2022-10-10 Mankei Tsang

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

One-shot full-field surface profilometry using digital diffractive-confocal imaging correlation microscope based on digital micromirror device is developed for one-shot microscopic 3D surface measurement. Optical configuration applies…

Optics · Physics 2019-04-24 Duc Trung Nguyen , Liang-Chia Chen , Nguyen Dinh Nguyen

Imaging with a layered superlens is a spatial filtering operation characterized by the point spread function (PSF). We show that in the same optical system the image of a narrow sub-wavelength Gaussian incident field may be surprisingly…

Optics · Physics 2010-04-08 Rafal Kotynski , Tomasz Stefaniuk

The structural complexity and instability of many interference phase microscopy methods are the major obstacles toward high-precision phase measurement. In this vein, improving more efficient configurations as well as proposing new methods…

Optics · Physics 2020-01-08 Samira Ebrahimi , Masoomeh Dashtdar

High-resolution optical microscopy suffers from a low contrast in scattering media where a multiply scattered wave obscures a ballistic wave used for image formation. To extend the imaging depth, various gating operations - confocal,…

Optics · Physics 2018-11-08 Mooseok Jang , Hakseok Ko , Won Kyu Lee , Jae-Seung Lee , Wonshik Choi

Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes /…

Optics · Physics 2020-10-29 Yujia Xue , Ian G. Davison , David A. Boas , Lei Tian

To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…

Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…