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Molecular dipoles near interfaces emit highly directional radiation due to near-field interactions, making surface-bound fluorophores sensitive probes of local physicochemical changes. We introduce smart coverslips, stably coated with…

Light diffraction through a subwavelength aperture located at the apex of a metallic screen with conical geometry is investigated theoretically. A method based on a multipole field expansion is developed to solve Maxwell's equations…

Optics · Physics 2010-02-15 A. Drezet , J. C. Woehl , S. Huant

Diffractive/refractive optics, such as Phase Fresnel Lenses (PFL's), offer the potential to achieve excellent imaging performance in the x-ray and gamma-ray photon regimes. In principle, the angular resolution obtained with these devices…

We present a new interferometric imaging approach that allows for multiple-depth imaging in a single acquisition, using off-axis low-coherence holographic multiplexing. This technique enables sectioned imaging of multiple slices within a…

Optics · Physics 2019-03-29 Lauren Wolbromsky , Nir A. Turko , Natan T. Shaked

Focal plane wavefront sensing and control is a critical approach to reducing non-common path errors between the a conventional astronomical adaptive optics (AO) wavefront sensor (WFS) detector and science camera. However, in addition to…

Instrumentation and Methods for Astrophysics · Physics 2023-03-01 Benjamin L. Gerard , Daren Dillon , Sylvain Cetre , Rebecca Jensen-Clem

Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing…

Image and Video Processing · Electrical Eng. & Systems 2026-02-20 Lukas Harris , Micah Roschelle , Jack Bartley , Mekhail Anwar

Super-resolution fluorescence microscopy overcomes blurring arising from light diffraction, allowing the reconstruction of fine scale details in biological structures. Standard methods come at the expense of long acquisition time and/or…

Optimization and Control · Mathematics 2021-10-12 Bastien Laville , Laure Blanc-Féraud , Gilles Aubert

Imaging through optical multimode fibers (MMFs) has the potential to enable hair-thin endoscopes that reduce the invasiveness of imaging deep inside tissues and organs. Current approaches predominantly require active wavefront shaping and…

Optics · Physics 2021-12-13 Szu-Yu Lee , Vicente J. Parot , Brett E. Bouma , Martin Villiger

We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism and structured illumination microscopy. In this method, the optical chirality of the illumination is structured and the…

Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…

Instrumentation and Detectors · Physics 2019-12-03 Gaelle Recher , Pierre Nassoy , Amaury Badon

A method for the analysis of superresolution microscopy images is presented. This method is based on the analysis of stochastic trajectories of particles moving on the membrane of a cell with the assumption that this motion is determined by…

Optimization and Control · Mathematics 2023-11-09 Mario Annunziato , Alfio Borzì

The conversion of phase variations in an optical wavefield into intensity information is of fundamental importance for optical imaging technology including microscopy of biological cells. While conventional approaches to phase-imaging…

Optics · Physics 2022-09-07 Lukas Wesemann , Jon Rickett , Timothy J. Davis , Ann Roberts

We introduce the concept of saturated absorption competition (SAC) microscopy as a means of providing sub-diffraction spatial resolution in fluorescence imaging. Unlike the post-competition process between stimulated and spontaneous…

Point spread function (PSF) plays an essential role in image reconstruction. In the context of confocal microscopy, optical performance degrades towards the edge of the field of view as astigmatism, coma and vignetting. Thus, one should…

Computer Vision and Pattern Recognition · Computer Science 2017-08-01 Anna Jezierska , Hugues Talbot , Jean-Christophe Pesquet , Gilbert Engler

Event-based image sensors provide microsecond temporal resolution but lack spectral discrimination, whereas diffractive spectral imagers encode wavelength information at conventional frame rates. We introduce a fluorescence microscopy…

Optics · Physics 2026-03-20 Richard G. Baird , Boyden Myers , Erik M. Jorgensen , Rajesh Menon

We present a physics-informed deep learning framework to address common limitations in Confocal Laser Scanning Microscopy (CLSM), such as diffraction limited resolution, noise, and undersampling due to low laser power conditions. The…

Materials Science · Physics 2025-01-27 Zaheer Ahmad , Junaid Shabeer , Usman Saleem , Tahir Qadeer , Abdul Sami , Zahira El Khalidi , Saad Mehmood

Fluorescence recovery after photobleaching (FRAP) measurements offer an important tool for analyzing diffusion and binding processes. Confocal scanning laser microscopes that are used in FRAP experiments bleach regions with a radially…

Subcellular Processes · Quantitative Biology 2009-03-09 George D Tsibidis

Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Sparse Aperture Masking (SAM) allows for high-contrast imaging at small inner working angles, however the performance is limited by the small throughput and the number of baselines. We present the concept and first lab results of…

Instrumentation and Methods for Astrophysics · Physics 2018-08-03 David S. Doelman , Peter Tuthill , Barnaby Norris , Michael J. Wilby , Emiel H. Por , Christoph U. Keller , Michael J. Escuti , Frans Snik

The scanning fiber endoscope (SFE), an ultra-small optical imaging device with a large field-of-view (FOV) for having a clear forward view into the interior of blood vessels, has great potential in the cardio-vascular disease diagnosis and…

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