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High refractive index semiconductor nanowires have recently been demonstrated experimentally as an efficient platform for enhancing the signal in fluorescence-based biosensors. Here, we study through modelling how a vertical GaP nanowire…

Optics · Physics 2025-02-04 Nicklas Anttu

Capturing biological specimens at large scales with sub-micron resolution is crucial for biomedical research, but conventional cameras often can't handle the pixel requirements. While most microscopes use motorized stages to move samples…

Optics · Physics 2025-03-28 Charlie Kersuzan , Aymerick Bazin , Amaury Badon

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

Diffraction limit is manifested in the loss of high spatial frequency information that results from decay of evanescent waves. As a result, conventional far-field optics yields no information about an object's subwavelength features. Here…

Optics · Physics 2012-01-24 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

We experimentally probe complex bio-photonic architecture of microstructures on the transparent insect wings by a simple, non-invasive, real time optical technique. A stable and reproducible far-field diffraction pattern in transmission was…

Current and future high contrast imaging instruments aim to detect exoplanets at closer orbital separations, lower masses, and/or older ages than their predecessors. However, continually evolving speckles in the coronagraphic science image…

Instrumentation and Methods for Astrophysics · Physics 2022-06-22 Benjamin L. Gerard , Daren Dillon , Sylvain Cetre , Rebecca Jensen-Clem

Diffraction microtomography in coherent light is foreseen as a promising technique to image transparent living samples in three dimensions without staining. Contrary to conventional microscopy with incoherent light, which gives…

Optics · Physics 2009-11-13 Stanislas Vertu , Jean-Jacques Delaunay , Olivier Haeberle

The random motion of molecules in living cells has consistently been reported to deviate from standard Brownian motion, a behavior coined as ``anomalous diffusion''. Fluorescence Correlation Spectroscopy (FCS) is a powerful method to…

Quantitative Methods · Quantitative Biology 2024-07-18 Nathan Quiblier , Jan-Michael Rye , Pierre Leclerc , Henri Truong , Abdelkrim Hannou , Laurent Héliot , Hugues Berry

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

This paper presents an innovative approach to computational acoustic imaging of biperiodic surfaces, exploiting the capabilities of an acoustic superlens to overcome the diffraction limit. We address the challenge of imaging physical…

Analysis of PDEs · Mathematics 2026-01-05 Yuliang Wang

High-numerical-aperture optical coherence tomography (OCT) enables sub-cellular imaging but faces a trade-off between lateral resolution and depth of focus. Computational refocusing can correct defocus in Fourier-domain OCT, yet its…

Optics · Physics 2026-05-22 Yue Zhu , Shuichi Makita , Naoki Fukutake , Yoshiaki Yasuno

Poor access to eye care is a major global challenge that could be ameliorated by low-cost, portable, and easy-to-use diagnostic technologies. Diffuser-based imaging has the potential to enable inexpensive, compact optical systems that can…

Medical Physics · Physics 2020-06-23 Yunzhe Li , Gregory N. McKay , Nicholas J. Durr , Lei Tian

Photoacoustic microscopy with large depth of focus is significant to the biomedical research. The conventional optical-resolution photoacoustic microscope (OR-PAM) suffers from limited depth of field (DoF) since the employed focused…

Image and Video Processing · Electrical Eng. & Systems 2020-09-07 Xianlin Song , Jianshuang Wei , Lingfang Song

Phase microscopy is an invaluable tool in the biosciences and in clinical diagnostics. The sensitivity of current phase microscopy techniques is optimized for one specific mean phase value and varies significantly across a given sample.…

Optics · Physics 2019-08-30 Thomas Juffmann , Andrés de los Ríos Sommer , Sylvain Gigan

Biological and biomedical samples are routinely examined using focused two-photon (2P) fluorescence microscopy due to its intrinsic axial sectioning and reduced out-of-focus bleaching. However, 2P imaging often requires excitation…

Confocal laser endomicroscopy (CLE) allow on-the-fly in vivo intraoperative imaging in a discreet field of view, especially for brain tumors, rather than extracting tissue for examination ex vivo with conventional light microscopy.…

Lensless illumination single-pixel imaging with a multicore fiber (MCF) is a computational imaging technique that enables potential endoscopic observations of biological samples at cellular scale. In this work, we show that this technique…

Signal Processing · Electrical Eng. & Systems 2023-07-18 Olivier Leblanc , Matthias Hofer , Siddharth Sivankutty , Hervé Rigneault , Laurent Jacques

The fields of view of Extremely Large Telescopes will contain vast numbers of spatial sampling elements (spaxels) as their Adaptive Optics systems approach the diffraction limit over wide fields. Since this will exceed the detection…

Instrumentation and Methods for Astrophysics · Physics 2015-05-13 G. J. Murray , J. R. Allington-Smith

Optical scattering presents a major obstacle to high resolution imaging in biological tissue and other turbid media. Conventional photoacoustic imaging can partially overcome this obstacle, enabling imaging of optical absorption in the…