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Multi-exposure image fusion aims to generate a single high-dynamic image by integrating images with different exposures. Existing deep learning-based multi-exposure image fusion methods primarily focus on spatial domain fusion, neglecting…

Computer Vision and Pattern Recognition · Computer Science 2023-12-19 Guang Yang , Jie Li , Xinbo Gao

High-throughput 3D quantitative phase imaging (QPI) in flow cytometry enables label-free, volumetric characterization of individual cells by reconstructing their refractive index (RI) distributions from multiple viewing angles during flow…

Computer Vision and Pattern Recognition · Computer Science 2025-09-08 Enze Ye , Wei Lin , Shaochi Ren , Yakun Liu , Xiaoping Li , Hao Wang , He Sun , Feng Pan

We demonstrate a significant resolution enhancement beyond the conventional limit in multiphoton microscopy (MPM) using saturated excitation of fluorescence. Our technique achieves super-resolved imaging by temporally modulating the…

White Light Interferometry (WLI) is a precise optical tool for measuring the 3D topography of microstructures. However, conventional WLI cannot capture the natural color of a sample's surface, which is essential for many microscale research…

Computer Vision and Pattern Recognition · Computer Science 2025-01-17 Shuo Chen , Yijin Li , Guofeng Zhang

The acquisition of high-resolution retinal fundus images with a large field of view (FOV) is challenging due to technological, physiological and economic reasons. This paper proposes a fully automatic framework to reconstruct retinal images…

Computer Vision and Pattern Recognition · Computer Science 2016-02-11 Thomas Köhler , Axel Heinrich , Andreas Maier , Joachim Hornegger , Ralf P. Tornow

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

We present an external interferometric setup that is able to simultaneously acquire three wavelengths of the same sample instance without scanning or multiple exposures. This setup projects onto the monochrome digital camera three off-axis…

The resolution of photoacoustic imaging deep inside scattering media is limited by the acoustic diffraction limit. In this work, taking inspiration from super-resolution imaging techniques developed to beat the optical diffraction limit, we…

Optics · Physics 2017-11-15 Sergey Vilov , Bastien Arnal , Emmanuel Bossy

Current 3D photoacoustic tomography (PAT) systems offer either high image quality or high frame rates but are not able to deliver high spatial and temporal resolution simultaneously, which limits their ability to image dynamic processes in…

Numerical Analysis · Mathematics 2016-12-21 Simon Arridge , Paul Beard , Marta Betcke , Ben Cox , Nam Huynh , Felix Lucka , Olumide Ogunlade , Edward Zhang

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

This paper introduces Spectral Incoherent Diffractive Imaging (SIDI) as a novel method for achieving dark-field imaging of nanostructures with heterogeneous oxidation states. With SIDI, shifts in photoemission profiles can be spatially…

Optics · Physics 2023-12-25 Tamme Wollweber , Kartik Ayyer

Versatile, sterically accessible imaging systems capable of in vivo rapid volumetric functional and structural imaging deep in the brain continue to be a limiting factor in neuroscience research. Towards overcoming this obstacle, we present…

Neurons and Cognition · Quantitative Biology 2018-05-10 Manish Kumar , Sandeep Kishore , Jordan Nasenbeny , David McLean , Yevgenia Kozorovitskiy

We investigate methods to calibrate the non-common path aberrations at an adaptive optics system having a wavefront-correcting device working at an extremely high resolution (larger than 150x150). We use focal-plane images collected…

Instrumentation and Methods for Astrophysics · Physics 2015-06-22 Visa Korkiakoski , Christoph U. Keller , Niek Doelman , Matthew Kenworthy , Gilles Otten , Michel Verhaegen

Super-resolution imaging with advanced optical systems has been revolutionizing technical analysis in various fields from biological to physical sciences. However, many objects are hidden by strongly scattering media such as rough wall…

Optics · Physics 2021-09-06 Dong Wang , Sujit Kumar Sahoo , Cuong Dang

Acousto-optic tomography (AOT) enables optical-contrast imaging deep inside scattering samples via localized ultrasound modulation of scattered light. However, the resolution of AOT is inherently limited by the ultrasound focus size,…

Optics · Physics 2018-12-12 Daniel Doktofsky , Moriya Rosenfeld , Ori Katz

Single-photon avalanche diodes (SPADs) are becoming popular in time-of-flight depth-ranging due to their unique ability to capture individual photons with picosecond timing resolution. However, ambient light (e.g., sunlight) incident on a…

Image and Video Processing · Electrical Eng. & Systems 2019-08-20 Anant Gupta , Atul Ingle , Mohit Gupta

Three-dimensional (3D) subcellular imaging is essential for biomedical research, but the diffraction limit of optical microscopy compromises axial resolution, hindering accurate 3D structural analysis. This challenge is particularly…

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

A robust method and strategy for efficient full field-ofview and depth separation optical imaging through scattering media regardless of the three-dimensional (3D) optical memory effect are proposed. In this method, the problem of imaging…

Optics · Physics 2020-01-17 Wei Li , Jietao Liu , Shunfu He , Lixian Liu , Xiaopeng Shao

Polarization microscopy provides insights into the structure and orientational organization of biomolecules and their architectures in cells. The above key functional signatures, which are natively 3D, can be only detected in 2D for a…