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Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit…

3D imaging is increasingly impacting areas such as space, defense, automation, medical and automotive industries. The most well-known optical 3D imaging systems are LIDAR systems that rely on Time of Flight (ToF) measurement. The depth…

Optics · Physics 2022-07-21 Koray Ürkmen , Emre Yüce

Multispectral imaging has been used for numerous applications in e.g., environmental monitoring, aerospace, defense, and biomedicine. Here, we present a diffractive optical network-based multispectral imaging system trained using deep…

Optics · Physics 2023-04-07 Deniz Mengu , Anika Tabassum , Mona Jarrahi , Aydogan Ozcan

The row-column addressed array is an emerging probe for ultrafast 3-D ultrasound imaging. It achieves this with far fewer independent electronic channels and a wider field of view than traditional 2-D matrix arrays, of the same channel…

In deep tissue photoacoustic imaging the spatial resolution is inherently limited by the acoustic wavelength. Recently, it was demonstrated that it is possible to surpass the acoustic diffraction limit by analyzing fluctuations in a set of…

Multi-focus image fusion (MFIF) addresses the depth-of-field (DOF) limitations of optical lenses, where only objects within a specific range appear sharp. Although traditional and deep learning methods have advanced the field, challenges…

Computer Vision and Pattern Recognition · Computer Science 2025-10-23 Luca Piano , Peng Huanwen , Radu Ciprian Bilcu

We report the development of a multichannel microscopy for whole-slide multiplane, multispectral, and phase imaging. We use trinocular heads to split the beam path into 6 independent channels and employ a camera array for parallel data…

Instrumentation and Detectors · Physics 2017-08-24 Jun Liao , Zhe Wang , Zibang Zhang , Zichao Bian , Kaikai Guo , Aparna Nambiar , Yutong Jiang , Shaowei Jiang , Jingang Zhong , Michael Choma , Guoan Zheng

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Hyperspectral imaging is an important tool having been applied in various fields, but still limited in observation of dynamic scenes. In this paper, we propose a snapshot hyperspectral imaging technique which exploits both spectral and…

Instrumentation and Detectors · Physics 2018-12-26 Chao Deng , Xuemei Hu , Jinli Suo , Yuanlong Zhang , Zhili Zhang , Qionghai Dai

Multi-spectral imaging, which simultaneously captures the spatial and spectral information of a scene, is widely used across diverse fields, including remote sensing, biomedical imaging, and agricultural monitoring. Here, we introduce a…

Spatial resolution of most imaging devices is fundamentally restricted by diffraction. This limitation is manifested in the loss of high spatial frequency information contained in evanescent waves. As a result, conventional far-field optics…

Optics · Physics 2010-03-16 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

Spatial frequency domain imaging (SFDI) is a low-cost imaging technique that can deliver real-time maps of absorption and reduced scattering coefficients. However, there are a wide range of imaging geometries that practical SFDI systems…

Image and Video Processing · Electrical Eng. & Systems 2023-02-27 Jane Crowley , George S. D. Gordon

Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC…

Quantitative Methods · Quantitative Biology 2019-02-06 Yuanyuan Han , Rui Tang , Yi Gu , Alex Ce Zhang , Wei Cai , Violet Castor , Sung Hwan Cho , William Alaynick , Yu-Hwa Lo

Confocal microscopy is the cornerstone of cellular biology and biomedical research due to its non-destructive imaging, compatibility with live cells, sensitivity, optical sectioning, and subcellular resolution. To meet the demand for rapid…

We present a new two-snapshot structured light illumination (SLI) reconstruction algorithm for fast image acquisition. The new algorithm, which only requires two mutually {\pi} phase-shifted raw structured images, is implemented on a…

Optics · Physics 2017-10-25 Yunlong Meng , Wei Lin , Chenglin Li , Shih-chi Chen

Spectral imaging enables the analysis of optical material properties that are invisible to the human eye. Different spectral capturing setups, e.g., based on filter-wheel, push-broom, line-scanning, or mosaic cameras, have been introduced…

Image and Video Processing · Electrical Eng. & Systems 2024-10-01 Eric L. Wisotzky , Jost Triller , Anna Hilsmann , Peter Eisert

In coherent diffractive imaging (CDI) the resolution of the reconstructed object is limited by the numerical aperture of the experimental setup. We present here a theoretical and numerical study for achieving super-resolution by…

Data Analysis, Statistics and Probability · Physics 2016-09-20 Tatiana Latychevskaia , Yuriy Chushkin , Hans-Werner Fink

Three dimensional Polarized Light Imaging (3D-PLI) is an optical technique which allows mapping the spatial fiber architecture of fibrous postmortem tissues, at sub-millimeter resolutions. Here, we propose an analytical and fast approach to…

Medical Physics · Physics 2020-12-18 A. Alimi , S. Deslauriers-Gauthier , F. Matuschke , A. Müller , S. E. A. Muenzing , M. Axer , R. Deriche

Our work aims at using quantitative imaging tools to complement the limitation of noise encountered by high resolution fluorescence microscopy methods. Several cycles of fluorophore activation, imaging and deactivation produce a sequence of…

Quantitative Methods · Quantitative Biology 2012-02-03 Juliane Liepe , Federico Felizzi , Agata Pernus , Maria Hanulova

To obtain microscope images at multiple focal planes, the distance between the objective and sample can be mechanically adjusted. Images are acquired sequentially at each axial distance. Digital refocusing with a light-emitting diode (LED)…

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