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Confocal and multiphoton microscopy are effective techniques to obtain high-contrast images of 2-D sections within bulk tissue. However, scattering limits their application to depths only up to ~1 millimeter. Multimode fibers make excellent…

Optics · Physics 2022-02-23 Sakshi Singh , Simon Labouesse , Rafael Piestun

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

Nonlinear structured illumination microscopy (nSIM) is an effective approach for super-resolution wide-field fluorescence microscopy with a theoretically unlimited resolution. In nSIM, carefully designed, highly-contrasted illumination…

Terahertz subwavelength imaging aims at developing THz microscopes able to resolve deeply subwavelength features. To improve the spatial resolution beyond the diffraction limit, a current trend is to use various subwavelength probes to…

Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…

Biological Physics · Physics 2016-08-04 Partha Pratim Mondal

Many biological and soft matter processes occur at high speeds in complex 3D environments, and developing imaging techniques capable of elucidating their dynamics is an outstanding experimental challenge. Here, we introduce Fourier…

Light field cameras have been proved to be powerful tools for 3D reconstruction and virtual reality applications. However, the limited resolution of light field images brings a lot of difficulties for further information display and…

Image and Video Processing · Electrical Eng. & Systems 2020-08-27 Qingyan Sun , Shuo Zhang , Song Chang , Lixi Zhu , Youfang Lin

We present SPIFFI, the integral field spectrometer for the VLT. This instrument allows simultaneous observation of infrared spectra in more than 1000 image points of a two dimensional field. With its set of four gratings and a pixel scale…

Astrophysics · Physics 2015-06-24 F. Eisenhauer , M. Tecza , S. Mengel , N. Thatte , C. Roehrle , K. Bickert , J. Schreiber

We present a deep learning driven computational approach to overcome the limitations of self-interference digital holography that imposed by inferior axial imaging performances. We demonstrate a 3D deep neural network model can…

Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method…

Optics · Physics 2025-09-11 Charlie Kersuzan , Anirban Jana , Amaury Badon

Mid-infrared hyperspectral imaging has become an indispensable tool to spatially resolve chemical information in a wide variety of samples. However, acquiring three-dimensional data cubes is typically time-consuming due to the limited speed…

Optics · Physics 2026-05-29 Jianan Fang , Kun Huang , Ruiyang Qin , Yan Liang , E Wu , Ming Yan , Heping Zeng

Camera calibration consists of estimating camera parameters such as the zenith vanishing point and horizon line. Estimating the camera parameters allows other tasks like 3D rendering, artificial reality effects, and object insertion in an…

Computer Vision and Pattern Recognition · Computer Science 2024-09-25 Sebastian Janampa , Marios Pattichis

3D multi-slab acquisitions are an appealing approach for diffusion MRI because they are compatible with the imaging regime delivering optimal SNR efficiency. In conventional 3D multi-slab imaging, shot-to-shot phase variations caused by…

Medical Physics · Physics 2024-11-26 Ziyu Li , Karla L. Miller , Xi Chen , Mark Chiew , Wenchuan Wu

Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds…

High spatio-angular resolution diffusion MRI (dMRI) has been shown to provide accurate identification of complex neuronal fiber configurations, albeit, at the cost of long acquisition times. We propose a method to recover intra-voxel fiber…

Image and Video Processing · Electrical Eng. & Systems 2021-12-07 Marica Pesce , Audrey Repetti , Anna Auría , Alessandro Daducci , Jean-Philippe Thiran , Yves Wiaux

This article experimentally examines different configurations of a novel multi-camera array microscope (MCAM) imaging technology. The MCAM is based upon a densely packed array of "micro-cameras" to jointly image across a large field-of-view…

Event-based image sensors provide microsecond temporal resolution but lack spectral discrimination, whereas diffractive spectral imagers encode wavelength information at conventional frame rates. We introduce a fluorescence microscopy…

Optics · Physics 2026-03-20 Richard G. Baird , Boyden Myers , Erik M. Jorgensen , Rajesh Menon

3D image display is essential for next-generation volumetric imaging; however, dense depth multiplexing for 3D image projection remains challenging because diffraction-induced cross-talk rapidly increases as the axial image planes get…

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels