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Capturing screens is now routine in our everyday lives. But the photographs of emissive displays are often influenced by the flicker-banding (FB), which is alternating bright%u2013dark stripes that arise from temporal aliasing between a…
Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust…
Patient-specific therapies require that cells be manufactured in multiple batches of small volumes, making it a challenge for conventional modes of quality control. The added complexity of inherent variability (even within batches)…
Theory predicts that with an ultrashort and extremely bright coherent X-ray pulse, a single diffraction pattern may be recorded from a large macromolecule, a virus, or a cell before the sample explodes and turns into a plasma. Here we…
Numerical simulation is used to characterise double potential step chronoamperometry at a microband electrode for a simple redox process A + e- goes to B, under conditions of full support such that diffusion is the only active form of mass…
Multi-photon microscopy is a powerful tool in biomolecular research. Less complex and more cost effective excitation light sources will make this technique accessible to a broader community. Especially semiconductor diode seeded fiber…
Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. We review here the recent…
Due to the confined focal length of optical sensors, focusing all objects in a scene with a single sensor is a difficult task. To handle such a situation, image fusion methods are used in multi-focus environment. Discrete Cosine Transform…
The optical diffraction limit, formulated by Abbe 140 years ago, imposes a bound on imaging resolution in classical optics. Over the last twenty years, many theoretical schemes have been presented for overcoming the diffraction barrier in…
We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…
In this paper we propose a very efficient method to fuse the unregistered multi-focus microscopical images based on the speed-up robust features (SURF). Our method follows the pipeline of first registration and then fusion. However, instead…
Fourier ptychography (FP) imaging, drawing on the idea of synthetic aperture, has been demonstrated as a potential approach for remote sub-diffraction-limited imaging. Nevertheless, the farthest imaging distance is still limited around 10 m…
Accessing the low-energy non-equilibrium dynamics of materials and their polaritons with simultaneous high spatial and temporal resolution has been a bold frontier of electron microscopy in recent years. One of the main challenges lies in…
Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…
Fluorescence microscopy is of vital importance for understanding biological function. However most fluorescence experiments are only qualitative inasmuch as the absolute number of fluorescent particles can often not be determined.…
Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…
Fluorescence microscopy, while being a key driver for progress in the life sciences, is also subject to technical limitations. To overcome them, computational multiplexing techniques have recently been proposed, which allow multiple…
Rapid, comprehensive, and accurate cell phenotyping without compromising viability, is crucial to many important biomedical applications, including stem-cell therapy, drug screening, and liquid biopsy. Typical image cytometry methods…
Sequential registering of fluorescence signals in conventional Excitation-Emission Matrices (EEMs), followed by modeling based on multilinear properties of the data, requires stable fluorophore concentrations throughout the acquisition of…
Femtosecond-scale ultrafast imaging is an essential tool for visualizing ultrafast dynamics in molecular biology, physical chemistry, atomic physics, and fluid dynamics. Pump-probe imaging and a streak camera are the most widely used…