xSCYTE: Express Single-frame Cytometer through Tomographic Phase
Abstract
Rapid, comprehensive, and accurate cell phenotyping without compromising viability, is crucial to many important biomedical applications, including stem-cell therapy, drug screening, and liquid biopsy. Typical image cytometry methods acquire two-dimensional (2D) fluorescence images, where the fluorescence labelling process may damage living cells, and the information from 2D images is not comprehensive enough for precise cell analysis. Although three-dimensional (3D) label-free image cytometry holds great promise, its high throughput development faces several technical challenges. Here, we report eXpress Single-frame CYtometer through Tomographic phasE (xSCYTE), which reconstructs 3D Refractive Index (RI) maps of cells with diffraction-limited resolution. With these high-speed and high-precision imaging capabilities empowered by artificial intelligence, we envision xSCYTE may open up many new avenues of biomedical investigations and industries, such as multi-omic assays and quality control during cellular therapeutic manufacturing.
Cite
@article{arxiv.2202.03627,
title = {xSCYTE: Express Single-frame Cytometer through Tomographic Phase},
author = {Baoliang Ge and Yanping He and Mo Deng and Md Habibur Rahman and Yijin Wang and Ziling Wu and Yongliang Yang and Cuifang Kuang and Chung Hong N. Wong and Michael K. Chan and Yi-Ping Ho and Liting Duan and Zahid Yaqoob and Peter T. C. So and George Barbastathis and Renjie Zhou},
journal= {arXiv preprint arXiv:2202.03627},
year = {2024}
}
Comments
Baoliang Ge, Yanping He, Mo Deng contributed equally to this work