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We report a novel microscopy platform, termed Multi-Aperture Fourier ptychographic microscopy (MA-FPM), capable of realizing gigapixel complex field images with large data acquisition bandwidths - in gigapixels per second. MA-FPM is a…

Optics · Physics 2020-10-14 Pavan Chandra Konda , Jonathan M. Taylor , Andrew R. Harvey

Fluorescence microscopic imaging in centimeter-deep tissue has been highly sought-after for many years because much interesting in vivo micro-information, such as microcirculation, tumor angiogenesis, and metastasis, may deeply locate in…

The physical and chemical environment inside cells is of fundamental importance to all life but has traditionally been difficult to determine on a subcellular basis. Here we combine cutting-edge genomically integrated FRET biosensing to…

Cell Behavior · Quantitative Biology 2020-10-22 Jack W Shepherd , Sarah Lecinski , Jasmine Wragg , Sviatlana Shashkova , Chris MacDonald , Mark C Leake

Experimental characterization of blood flow in living organisms is crucial for understanding the development and function of cardiovascular systems, but there have been no techniques reported for snapshot imaging of thick samples in large…

A real-time ranging lidar with 0.1 Mega Hertz update rate and few-micrometer resolution incorporating dispersive Fourier transformation and instantaneous microwave frequency measurement is proposed and demonstrated. As time-stretched…

Signal Processing · Electrical Eng. & Systems 2020-08-06 Lijie Zhao , Haiyun Xia , Yihua Hu , Tengfei Wu , Zhen Zhang , Jibo Han , Yunbin Wu , Tiancheng Luo

Fluorescence is a powerful mean to probe information processing in the mammalian brain. However, neuronal tissues are highly heterogeneous and thus opaque to light. A wide set of non-invasive or invasive techniques for scattered light…

Optics · Physics 2020-05-11 Claudio Moretti , Sylvain Gigan

We present a technique for atomic density measurements by the off-resonant phase-shift induced on a two-frequency, coherently-synthesised light beam. We have used this scheme to measure the column density of a magnetically trapped atom…

Quantum Physics · Physics 2013-08-19 M. Kohnen , P. G. Petrov , R. A. Nyman , E. A. Hinds

Recently, diffusion models have shown remarkable results in image synthesis by gradually removing noise and amplifying signals. Although the simple generative process surprisingly works well, is this the best way to generate image data? For…

Computer Vision and Pattern Recognition · Computer Science 2022-11-22 Sangyun Lee , Hyungjin Chung , Jaehyeon Kim , Jong Chul Ye

Super-resolution light microscopy overcomes the physical barriers due to light diffraction, allowing for the observation of otherwise indistinguishable subcellular entities. However, the specific acquisition conditions required by…

Standard frame-based cameras that sample light intensity frames are heavily impacted by motion blur for high-speed motion and fail to perceive scene accurately when the dynamic range is high. Event-based cameras, on the other hand, overcome…

Computer Vision and Pattern Recognition · Computer Science 2021-03-22 Chankyu Lee , Adarsh Kumar Kosta , Kaushik Roy

Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…

In this paper, we propose an efficient, fast, and versatile distillation method to accelerate the generation of pre-trained diffusion models: Flash Diffusion. The method reaches state-of-the-art performances in terms of FID and CLIP-Score…

Computer Vision and Pattern Recognition · Computer Science 2024-12-19 Clément Chadebec , Onur Tasar , Eyal Benaroche , Benjamin Aubin

Measuring subdiffraction separations between single fluorescent particles is important for biological, nano-, and medical-technology studies. Major challenges include (i) measuring changing molecular separations with high temporal…

Biological Physics · Physics 2015-05-18 Shawn H. DeCenzo , Michael C. DeSantis , Y. M. Wang

The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

Optics · Physics 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with…

Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Optical phase-space functions describe spatial and angular information simultaneously; examples include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration…

Optics · Physics 2017-08-02 Hsiou-Yuan Liu , Jingshan Zhong , Laura Waller

Ptychography is a popular imaging technique that combines diffractive imaging with scanning microscopy. The technique consists of a coherent beam that is scanned across an object in a series of overlapping positions, leading to reliable and…

Numerical Analysis · Mathematics 2024-08-08 Ricardo Parada , Samy Wu Fung , Stanley Osher

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

Deep, high-resolution imaging is essential for unraveling biological complexity and advancing medical diagnostics, yet scattering fundamentally limits optical methods. Among the most promising approaches, photoacoustic imaging achieves…

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