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The resolution of optical imaging is classically limited by the width of the point-spread function, which in turn is determined by the Rayleigh length. Recently, spatial-mode demultiplexing (SPADE) has been proposed as a method to achieve…

Quantum Physics · Physics 2025-02-26 Giuseppe Buonaiuto , Cosmo Lupo

Single-molecule localization microscopy (SMLM) allows reconstructing biology-relevant structures beyond the diffraction limit by detecting and localizing individual fluorophores -- fluorescent molecules stained onto the observed specimen --…

Computer Vision and Pattern Recognition · Computer Science 2026-03-03 Romain Seailles , Jean-Baptiste Masson , Jean Ponce , Julien Mairal

Diffusion models have recently achieved remarkable performance in image super-resolution (SR), but their high computational cost limits practical deployment in remote sensing applications. To address this issue, we propose SlimDiffSR, a…

Computer Vision and Pattern Recognition · Computer Science 2026-05-19 Ce Wang , Zhenyu Hu , Wanjie Sun

Blind image separation (BIS) refers to the inverse problem of simultaneously estimating and restoring multiple independent source images from a single observation image under conditions of unknown mixing mode and without prior knowledge of…

Computer Vision and Pattern Recognition · Computer Science 2026-02-17 Jingwei Li , Wei Pu

We propose a single-snapshot depth-from-defocus (DFD) reconstruction method for coded-aperture imaging that replaces hand-crafted priors with a learned diffusion prior used purely as regularization. Our optimization framework enforces…

Computer Vision and Pattern Recognition · Computer Science 2025-09-29 Hodaka Kawachi , Jose Reinaldo Cunha Santos A. V. Silva Neto , Yasushi Yagi , Hajime Nagahara , Tomoya Nakamura

Single-shot fluorescence imaging techniques have gained increasing interest in recent years due to their ability to rapidly capture complex biological data without the need for extensive scanning. In this letter, we introduce polarized…

Optics · Physics 2025-01-31 Oumeng Zhang , Changhuei Yang

Super-resolution localization microscopy is based on determining the positions of individual fluorescent markers in a sample. The major challenge in reaching an ever higher localization precision lies in the limited number of collected…

Optics · Physics 2020-08-05 Daniel Böning , Franz-Ferdinand Wieser , Vahid Sandoghdar

Structure from Motion (SfM) often fails to estimate accurate poses in environments that lack suitable visual features. In such cases, the quality of the final 3D mesh, which is contingent on the accuracy of those estimates, is reduced. One…

Computer Vision and Pattern Recognition · Computer Science 2021-04-08 Victor Amblard , Timothy P. Osedach , Arnaud Croux , Andrew Speck , John J. Leonard

The speed and quality of single-pixel imaging (SPI) are fundamentally limited by image modulation frequency and by the levels of optical noise and compression noise. In an approach to come close to these limits, we introduce a SPI…

Optics · Physics 2022-05-25 Anna Pastuszczak , Rafał Stojek , Piotr Wróbel , Rafał Kotyński

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

We present a simple method to measure the radiative and non-radiative recombination rates of individual fluorescent emitters at room temperature. By placing a single molecule successively close and far from a dielectric interface and…

Other Condensed Matter · Physics 2009-03-14 X. Brokmann , L. Coolen , Maxime Dahan , J. P. Hermier

Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…

Image and Video Processing · Electrical Eng. & Systems 2022-10-20 Abhishek Mall , Kartik Ayyer

In single-molecule super-resolution microscopy, engineered point-spread functions (PSFs) are designed to efficiently encode new molecular properties, such as 3D orientation, into complex spatial features captured by a camera. To fully…

Image and Video Processing · Electrical Eng. & Systems 2019-07-16 Hesam Mazidi , Eshan S. King , Oumeng Zhang , Arye Nehorai , Matthew D. Lew

Image sensors are the backbone of many imaging technologies of great importance to modern sciences, being particularly relevant in biomedicine. An ideal image sensor should be usable through all the electromagnetic spectrum (large…

Medical Physics · Physics 2022-03-16 Juan Aguirre

SSD (Single Shot Multibox Detector) is one of the most successful object detectors for its high accuracy and fast speed. However, the features from shallow layer (mainly Conv4_3) of SSD lack semantic information, resulting in poor…

Computer Vision and Pattern Recognition · Computer Science 2021-08-26 Hao Zhang , Xianggong Hong , Li Zhu

Super-resolution is the process of obtaining a high-resolution image from one or more low-resolution images. Single image super-resolution (SISR) and multi-frame super-resolution (MFSR) methods have been evolved almost independently for…

Computer Vision and Pattern Recognition · Computer Science 2023-03-07 Mohammad Mahdi Afrasiabi , Reshad Hosseini , Aliazam Abbasfar

Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…

Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar…

We propose a holographic microscopy method based on single-pixel imaging technology (HM-SPI). We used a holographic microscopy method based on in-line Gabor holography. In single-pixel imaging technology, cyclic binary masks and…

Optics · Physics 2025-04-11 Arthur G. Fedorov

Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…