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Super-resolution microscopy normally requiring complex and cumbersome optics is not applicable for in situ imaging through a narrow channel. Here, we demonstrate single hair-thin multimode fiber (MMF) endoscope (less than 250 ${\mu}m$) for…

Fluorescence microscopy is a key driver to promote discoveries of biomedical research. However, with the limitation of microscope hardware and characteristics of the observed samples, the fluorescence microscopy images are susceptible to…

Image and Video Processing · Electrical Eng. & Systems 2022-09-15 Xuanyu Tian , Qing Wu , Hongjiang Wei , Yuyao Zhang

Time-resolved single photon imaging is a promising imaging modality characterized by the unique capability of timestamping the arrivals of single photons. Single-Photon Avalanche Diodes (SPADs) are the leading technology for implementing…

Computer Vision and Pattern Recognition · Computer Science 2024-10-23 Aleksi Suonsivu , Lauri Salmela , Edoardo Peretti , Leevi Uosukainen , Radu Ciprian Bilcu , Giacomo Boracchi

High-speed multiplex imaging of fluorescent probes is limited by a combination of spectral resolution, sensitivity, high cost and low light throughput of detectors, and filters. In this work, we present a hyperspectral detection system…

The most significant problem may be undesirable effects for the spectral signatures of fused images as well as the benefits of using fused images mostly compared to their source images were acquired at the same time by one sensor. They may…

Computer Vision and Pattern Recognition · Computer Science 2012-08-27 Firouz Abdullah Al-Wassai , N. V. Kalyankar

A new procedure aiming at folding a powder diffraction 2-D into a 1-D scan is presented. The technique consists of three steps: tracking the beam centre by means of a Simulated Annealing (SA) of the diffraction rings along the same axis,…

Materials Science · Physics 2008-12-02 Antonio Cervellino , Cinzia Giannini , Antonietta Guagliardi , Massimo Ladisa

Images obtained by microscopes are generally degraded by aberrations. Adaptive optics (AO) has been widely used in to compensate for aberrations and improve reduced image quality. Requiring no separate wavefront sensor, sensorless AO…

Optics · Physics 2025-09-23 Biwei Zhang , Qi Hu , Jingyu Wang , Martin J. Booth

Precise manipulation of single molecules has already led to remarkable insights in physics, chemistry, biology and medicine. However, widespread adoption of single-molecule techniques has been impeded by equipment cost and the laborious…

Biological Physics · Physics 2015-05-14 Ken Halvorsen , Wesley P. Wong

We report the modification of a label-free image scanning microscope (ISM) to perform asynchronous 2D imaging at 24kHz while keeping the lateral resolution gain and background rejection of a regular label-free ISM setup. Our method uses a…

Optics · Physics 2023-08-31 Duc-Minh Ta , Alberto Aguilar , Pierre Bon

Comparing images captured by disparate sensors is a common challenge in remote sensing. This requires image translation -- converting imagery from one sensor domain to another while preserving the original content. Denoising Diffusion…

Computer Vision and Pattern Recognition · Computer Science 2024-12-05 João Gabriel Vinholi , Marco Chini , Anis Amziane , Renato Machado , Danilo Silva , Patrick Matgen

In this work, we present a novel technique to directly measure the phase shift of the optical signal scattered by single plasmonic nanoparticles in a diffraction-limited laser focus. We accomplish this by equipping an inverted confocal…

Hyperspectral cameras face harsh trade-offs between spatial, spectral, and temporal resolution in inherently low-photon conditions. Computational imaging systems break through these trade-offs with compressive sensing, but have required…

Computer Vision and Pattern Recognition · Computer Science 2026-02-12 M. Kerem Aydin , Yi-Chun Hung , Jaclyn Pytlarz , Qi Guo , Emma Alexander

Spectroscopy is a well-established nonintrusive tool that has played an important role in identifying substances and quantifying their compositions, from quantum descriptions to chemical and biomedical diagnostics. Challenges exist in…

Atmospheric and Oceanic Physics · Physics 2020-12-02 S. Yu , Z. Zhang , H. Xia , X. Dou , M. Li , T. Wei , L. Wang , P. Jiang , Y. Wu , C. Zhang , L. You , Y. Hu , T. Wu , L. Zhao , M. Shangguan , L. Tao , J. Qiu

A novel multi-focus image fusion algorithm performed in spatial domain based on similarity characteristics is proposed incorporating with region segmentation. In this paper, a new similarity measure is developed based on the structural…

Computer Vision and Pattern Recognition · Computer Science 2022-02-01 Ya-Qiong Zhang , Xiao-Jun Wu , Hui Li

We present a method to separate a single image captured under two illuminants, with different spectra, into the two images corresponding to the appearance of the scene under each individual illuminant. We do this by training a deep neural…

Computer Vision and Pattern Recognition · Computer Science 2019-04-24 Zhuo Hui , Ayan Chakrabarti , Kalyan Sunkavalli , Aswin C. Sankaranarayanan

Fluorescence spectroscopy is an image correlation technique to analyze and characterize the molecular dynamics from a sequence of fluorescence images. Many image correlation techniques have been developed for different applications [1]. But…

Quantitative Methods · Quantitative Biology 2017-10-24 C. Chen , P. Paul-Gilloteaux , T. Vignaud , J. Salamero , F. Waharte

Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

Single particle reconstruction (SPR) from cryo-electron microscopy (EM) is a technique in which the 3D structure of a molecule needs to be determined from its contrast transfer function (CTF) affected, noisy 2D projection images taken at…

Applications · Statistics 2017-06-30 Tejal Bhamre , Zhizhen Zhao , Amit Singer

In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has…

Distinguishing photon-arrival time and position is crucial for advancing quantum technology. However, capturing spatial and temporal information efficiently remains challenging. Here, we present a novel photon-detection technique to achieve…

Quantum Physics · Physics 2024-07-24 Ozora Iso , Kensuke Miyajima , Ryosuke Shimizu