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Structured illumination in Single Molecule Localization Microscopy provides new information on the position of molecules and thus improves the localization precision compared to standard localization methods. Here, we used a time-shifted…

A new method to tag the barium daughter in the double beta decay of $^{136}$Xe is reported. Using the technique of single molecule fluorescent imaging (SMFI), individual barium dication (Ba$^{++}$) resolution at a transparent scanning…

Self-supervised monocular depth estimation (SSMDE) has gained attention in the field of deep learning as it estimates depth without requiring ground truth depth maps. This approach typically uses a photometric consistency loss between a…

Computer Vision and Pattern Recognition · Computer Science 2025-03-31 Wonhyeok Choi , Kyumin Hwang , Minwoo Choi , Kiljoon Han , Wonjoon Choi , Mingyu Shin , Sunghoon Im

Ptychography has become prominent at synchrotron facilities worldwide for characterizing biological and material specimens' topological structures and properties at the nanometer or atomic scale, due to its lens - less, highly quantitative…

Image and Video Processing · Electrical Eng. & Systems 2025-01-28 Tao Liu , Bingyang Wang , JiangTao Zhao , Maik Kahnt , Fucai Zhang

Single Molecule Localization Microscopy (SMLM) enables the acquisition of high-resolution images by alternating between activation of a sparse subset of fluorescent molecules present in a sample and localization. In this work, the…

Image and Video Processing · Electrical Eng. & Systems 2018-12-17 Arne Bechensteen , Laure Blanc-Féraud , Gilles Aubert

Molecule-surface interaction is key to many physical and chemical processes at interfaces. Here, we show that the dynamics of single molecules on a surface under ultrahigh vacuum can be resolved using fluorescence imaging. By adapting…

Chemical Physics · Physics 2021-10-19 Tianyu Fang , Florian Elsen , Nick Vogeley , Daqing Wang

Multi-spectral imaging, which simultaneously captures the spatial and spectral information of a scene, is widely used across diverse fields, including remote sensing, biomedical imaging, and agricultural monitoring. Here, we introduce a…

The non-separability between the spatial and polarisation Degrees of Freedom (DoFs) of complex vector light fields has drawn significant attention in recent time. Key to this are its remarkable similarities with quantum entanglement, with…

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

Optics · Physics 2024-03-26 Tingting Wu , Matthew D. Lew

In this work, we study the conditions under which fluorescent beads can be used to emulate single fluorescent molecules in the calibration of optical microscopes. Although beads are widely used due to their brightness and easy manipulation,…

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely…

Optics · Physics 2025-01-17 David Alonso , Javier Garcia , Vicente Mico

Single-mode optical nanofibres are a central component of a broad range of applications and emerging technologies. Their fabrication has been extensively studied over the past decade, but imaging of the final sub-micrometre products has…

Imaging across both the full transverse spatial and temporal dimensions of a scene with high precision in all three coordinates is key to applications ranging from LIDAR to fluorescence lifetime imaging. However, compromises that sacrifice,…

Image and Video Processing · Electrical Eng. & Systems 2021-01-12 C. Callenberg , A. Lyons , D. den Brok , A. Fatima , A. Turpin , V. Zickus , L. Machesky , J. Whitelaw , D. Faccio , M. B. Hullin

We present a compact, diffuser-assisted, single-pixel computational camera. A rotating ground glass diffuser is adopted, in preference to a commonly used digital micro-mirror device (DMD), to encode a two-dimensional (2D) image into…

Optics · Physics 2021-11-04 Baolei Liu , Fan Wang , Chaohao Chen , David McGloin

We propose a method to monochromatize multiple orders of high harmonics by using a proper designed multilayer mirror. Multilayer mirrors designed by our concept realize the perfect extraction of a single-order harmonic from multiple-order…

Super-resolution optical fluctuation imaging (SOFI) provides a resolution beyond the diffraction limit by analysing stochastic fluorescence fluctuations with higher-order statistics. Using nth order spatio-temporal cross-cumulants the…

Lensless illumination single-pixel imaging with a multicore fiber (MCF) is a computational imaging technique that enables potential endoscopic observations of biological samples at cellular scale. In this work, we show that this technique…

Signal Processing · Electrical Eng. & Systems 2023-07-18 Olivier Leblanc , Matthias Hofer , Siddharth Sivankutty , Hervé Rigneault , Laurent Jacques

Super-resolution imaging using sub-diffraction field localization by micron sized transparent beads (microspheres) was recently demonstrated [1]. Practical applications in microscopy require control over the positioning of the microspheres.…

Optics · Physics 2013-12-25 Leonid A. Krivitsky , Jia Jun Wang , Zengbo Wang , Boris Lukiyanchuk
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