English
Related papers

Related papers: Single-image measurements of monochromatic subdiff…

200 papers

The Depth from Defocus (DFD) imaging technique for measuring the size and number concentration of particles in a dispersed two-phase flow has up to now been restricted to relatively sparse particle densities and to identifying only…

Fluid Dynamics · Physics 2024-04-01 Rixin Xua , Zuojie Huanga , Wenchao Gonga , Wu Zhoua , Cameron Tropea

Advancement in mid-infrared (MIR) technology has led to promising biomedical applications of MIR spectroscopy, such as liquid biopsy or breath diagnosis. On the contrary, MIR microscopy has been rarely used for live biological samples in an…

Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…

Optics · Physics 2021-02-02 Oumeng Zhang , Matthew D. Lew

Mid-infrared (MIR) spectroscopy is widely recognized as a powerful, non-distractive method for chemical analysis. However, its utility is constrained by a micrometer-scale spatial resolution imposed by the long-wavelength MIR diffraction…

In multi-photon microscopy (MPM), a recent in-vivo fluorescence microscopy system, the task of image restoration can be decomposed into two interlinked inverse problems: firstly, the characterization of the Point Spread Function (PSF) and…

Image and Video Processing · Electrical Eng. & Systems 2024-11-08 Julien Ajdenbaum , Emilie Chouzenoux , Claire Lefort , Ségolène Martin , Jean-Christophe Pesquet

The wavefront measurement of a light beam is a complex task, which often requires a series of spatially resolved intensity measurements. For instance, a detector array may be used to measure the local phase gradient in the transverse plane…

The advent of single molecule optics has had a profound impact in fields ranging from biophysics to material science, photophysics, and quantum optics. However, all existing room-temperature single molecule methods have been based on…

Mesoscale and Nanoscale Physics · Physics 2010-07-22 Michele Celebrano , Philipp Kukura , Alois Renn , Vahid Sandoghdar

Silicon Photomultipliers (SiPMs) are the state-of-the-art technology in single-photon detection with solid-state detectors. Single Photon Avalanche Diodes (SPADs), the key element of SiPMs, can now be manufactured in CMOS processes,…

Super-resolution fluorescence microscopy is of great interest in life science studies for visualizing subcellular structures at the nanometer scale. Among various kinds of super-resolution approaches, image scanning microscopy (ISM) offers…

Mid-infrared (MIR) imaging offers powerful capabilities for label-free chemical analysis, yet its practical deployment remains hindered by the high cost and cryogenic complexity of conventional cameras. Two-photon absorption (TPA) provides…

Optics · Physics 2026-05-25 Huijie Ma , Kun Huang , Jianan Fang , Ziyu He , Yan Liang , Heping Zeng

Room temperature detection of molecular vibrations in the mid-infrared (MIR, $\lambda$ =3-30$\mu$m) has numerous applications including real-time gas sensing, chemical reactivity, medical imaging, astronomical surveys, and quantum…

Applied Physics · Physics 2022-05-17 Rohit Chikkaraddy , Rakesh Arul , Lukas A. Jakob , Jeremy J. Baumberg

The movement of molecules inside living cells is a fundamental feature of biological processes. The ability to both observe and analyse the details of molecular diffusion in vivo at the single molecule and single cell level can add…

Quantitative Methods · Quantitative Biology 2012-11-21 Alex Robson , Kevin Burrage , Mark Leake

Diffuse optical imaging (DOI) offers valuable insights into scattering mediums, but the quest for high-resolution imaging often requires dense sampling strategies, leading to higher imaging errors and lengthy acquisition times. This work…

Optics · Physics 2025-04-07 Ben Wiesel , Shlomi Arnon

Purpose: Microscopic fractional anisotropy ({\mu}FA) can disentangle microstructural information from orientation dispersion. While double diffusion encoding (DDE) MRI methods are widely used to extract accurate {\mu}FA, it has only…

Medical Physics · Physics 2019-03-21 Rafael Neto Henriques , Sune N Jespersen , Noam Shemesh

Endoscopic optical imaging using a single multimode fiber (MMF) has emerged as a promising approach for highly compact, minimally invasive, and high-resolution imaging. Unlike conventional fiber bundles, MMF-based endomicroscopes exploit…

Moir\'e fringes are used throughout a wide variety of applications in physics and engineering to bring out small variations in an underlying lattice by comparing with another reference lattice. This method was recently demonstrated in…

Applied Physics · Physics 2020-02-06 Viveksharma Prabhakara , Daen Jannis , Armand Béché , Hugo Bender , Johan Verbeeck

Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…

Biomolecules · Quantitative Biology 2015-04-15 Mark Leake

Single molecule localization microscopy is a recently developed superresolution imaging technique to visualize structural properties of single cells. The basic principle consists in chemically attaching fluorescent dyes to the molecules,…

Single-molecule fluorescence spectroscopy is a powerful method that avoids ensemble averaging, but its temporal resolution is limited by the fluorescence lifetime to nanoseconds at most. At the ensemble level, two-dimensional spectroscopy…

Optics · Physics 2024-10-11 Sanchayeeta Jana , Simon Durst , Markus Lippitz

Scalable atom-based quantum platforms for simulation, computing, and metrology require fast high-fidelity, low-loss imaging of individual atoms. Standard fluorescence detection methods rely on continuous cooling, limiting the detection…