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We closely replicated and extended a recent experiment ("Spatial properties of entangled two-photon absorption," Phys. Rev. Lett. 129, 183601, 2022) that reportedly observed enhancement of two-photon absorption rates in molecular samples by…

Quantum Physics · Physics 2024-04-26 Tiemo Landes , Brian J. Smith , Michael G. Raymer

Imaging of rapidly occurring events in biology requires high temporal resolution. In this review article, I have tried to investigate an approximate estimate to quantify temporal resolution limit of a fluorescence imaging system. It was…

Medical Physics · Physics 2014-07-25 Partha Pratim Mondal

In this article, we derive the limit of detection for a two-step molecular recognition process and show that in-spite of all the recognition reactions being in equilibrium the overall error rates can be reduced exactly as much as possible…

Medical Physics · Physics 2019-07-11 Tuhin Chakrabortty , Manoj M Varma

The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein…

Biological Physics · Physics 2020-06-17 Kristin S. Grußmayer , Klaus Yserentant , Dirk-Peter Herten

Nowadays, Machine Learning (ML) is experiencing tremendous popularity that has never been seen before. The operationalization of ML models is governed by a set of concepts and methods referred to as Machine Learning Operations (MLOps).…

Machine Learning · Computer Science 2023-10-05 Angelo Yamachui Sitcheu , Nils Friederich , Simon Baeuerle , Oliver Neumann , Markus Reischl , Ralf Mikut

A novel algorithm is proposed for segmenting an image into multiple levels using its mean and variance. Starting from the extreme pixel values at both ends of the histogram plot, the algorithm is applied recursively on sub-ranges computed…

Computer Vision and Pattern Recognition · Computer Science 2007-05-23 Siddharth Arora , Jayadev Acharya , Amit Verma , Prasanta K. Panigrahi

In recent years several methods to overcome diffraction limit in the far field microscopy have been demonstrated. Still the problem of superresolution is reliably solved only for fluorescent microscopy, giving a resolution of up to 20-30nm.…

Optics · Physics 2013-09-03 Yu. V. Miklyaev , S. A. Asselborn , K. A. Zaytsev , M. Ya. Darscht

In recent years several far-field microscopy techniques have been developed which manage to overcome the diffraction limit of resolution. A unifying classification scheme for them is clearly desirable. We argue that existing schemes based…

Optics · Physics 2016-06-17 Oliver Passon , Johannes Grebe-Ellis

Photonic RF transversal signal processors, which are equivalent to reconfigurable electrical digital signal processors but implemented with photonic technologies, have been widely used for modern high-speed information processing. With the…

Optics · Physics 2023-04-11 David J. Moss

In recent years, deep learning networks have made remarkable strides in the domain of multi-exposure image fusion. Nonetheless, prevailing approaches often involve directly feeding over-exposed and under-exposed images into the network,…

Computer Vision and Pattern Recognition · Computer Science 2024-04-11 Pan Mu , Zhiying Du , Jinyuan Liu , Cong Bai

Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies…

Clinical biosensors with low detection limit hold significant promise in the early diagnosis of debilitating diseases. Recent progress in sensor development has led to the demonstration of detection capable of detecting target molecules…

Medical Physics · Physics 2019-12-05 Tuhin Chakrabortty , Chandra R. Murthy , Manoj Varma

Advances in photo editing and manipulation tools have made it significantly easier to create fake imagery. Learning to detect such manipulations, however, remains a challenging problem due to the lack of sufficient amounts of manipulated…

Computer Vision and Pattern Recognition · Computer Science 2018-09-07 Minyoung Huh , Andrew Liu , Andrew Owens , Alexei A. Efros

Quality of image always plays a vital role in in-creasing object recognition or classification rate. A good quality image gives better recognition or classification rate than any unprocessed noisy images. It is more difficult to extract…

Computer Vision and Pattern Recognition · Computer Science 2020-11-16 Md Tanzil Shahriar , Huyue Li

Unsupervised deep image prior (DIP) addresses shortcomings of training data requirements and limited generalization associated with supervised deep learning. The performance of DIP depends on the network architecture and the stopping point…

In fluorescence diffuse optical tomography (fDOT), the reconstruction of the fluorophore concentration inside the target body is usually carried out using a normalized Born approximation model where the measured fluorescent emission data is…

Computational Physics · Physics 2015-01-05 Meghdoot Mozumder , Tanja Tarvainen , Simon Arridge , Jari P. Kaipio , Cosimo d'Andrea , Ville Kolehmainen

Traditional image acquisition for cryo focused ion-beam scanning electron microscopy tomography often sees thousands of images being captured over a period of many hours, with immense data sets being produced. When imaging beam sensitive…

Although many advances have been made in light-field and camera-array image processing, there is still a lack of thorough analysis of the localisation accuracy of different multi-camera systems. By considering the problem from a…

Computer Vision and Pattern Recognition · Computer Science 2016-02-25 Alireza Ghasemi , Adam Scholefield , Martin Vetterli

Fluorescence spectroscopy is an image correlation technique to analyze and characterize the molecular dynamics from a sequence of fluorescence images. Many image correlation techniques have been developed for different applications [1]. But…

Quantitative Methods · Quantitative Biology 2017-10-24 C. Chen , P. Paul-Gilloteaux , T. Vignaud , J. Salamero , F. Waharte

Image distortion correction is a critical pre-processing step for a variety of computer vision and image processing algorithms. Standard real-time software implementations are generally not suited for direct hardware porting, so…

Computer Vision and Pattern Recognition · Computer Science 2016-11-01 Paolo Di Febbo , Stefano Mattoccia , Carlo Dal Mutto