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Because of the double-helical structure of DNA, in which two strands of complementary nucleotides intertwine around each other, a covalently closed DNA molecule with no interruptions in either strand can be viewed as two interlocked…

Biomolecules · Quantitative Biology 2015-05-13 Lauren A. Britton , Wilma K. Olson , Irwin Tobias

In mechanical manipulation experiments, a single DNA molecule overwound at constant force undergoes a discontinuous drop in extension as it buckles and forms a superhelical loop (a plectoneme). Further overwinding the DNA, we observe an…

Biological Physics · Physics 2018-04-23 Andrew Dittmore , Keir C. Neuman

DNA supercoiling, the under or overwinding of DNA, is a key physical mechanism both participating to compaction of bacterial genomes and making genomic sequences adopt various structural forms. DNA supercoiling may lead to the formation of…

Biological Physics · Physics 2019-06-26 Thibaut Lepage , Ivan Junier

A stretched DNA molecule which is also under- or overwound, undergoes a buckling transition forming intertwined looped domains called plectonemes. Here we develop a simple theory that extends the two-phase model of stretched supercoiled…

Soft Condensed Matter · Physics 2024-11-01 Midas Segers , Enrico Skoruppa , Helmut Schiessel , Enrico Carlon

Motivated by recent experiments on DNA torsion-force-extension characteristics we consider the writhing geometry of open stiff molecules. We exhibit a cyclic motion which allows arbitrarily large twisting of the end of a molecule via an…

Biological Physics · Physics 2009-09-07 V. Rossetto , A. C. Maggs

In this work, we combine coarse-grained Brownian dynamics simulations and mean-field theory to study supercoiling dynamics, as well as the steady-state profiles of twist and writhe, in an open DNA polymer where one of the free ends is…

Soft Condensed Matter · Physics 2026-02-17 Daniela Moretti , Giuseppe Gonnella , Antonio Suma , Giada Forte , Davide Marenduzzo , Cristian Micheletti

Here we present a systematic study of supercoil formation in DNA minicircles under varying linking number by using molecular dynamics simulations of a two-bead coarse-grained model. Our model is designed with the purpose of simulating long…

Biological Physics · Physics 2015-05-14 Mehmet Sayar , Baris Avsaroglu , Alkan Kabakcioglu

Motivated by experiments in which single DNA molecules are stretched and twisted we consider a perturbative approach around very high forces, where we determine the writhe distribution in a simple, analytically tractable model. Our results…

Soft Condensed Matter · Physics 2009-11-10 Supurna Sinha

We examine the conformations of a model for under- and overwound DNA. The molecule is represented as a cylindrically symmetric elastic string subjected to a stretching force and to constraints corresponding to a specification of the link…

Soft Condensed Matter · Physics 2009-10-28 Boris Fain , Joseph Rudnick , Stellan Ostlund

The key finding in the DNA double helix model is the specific pairing or binding between nucleotides A-T and C-G, and the pairing rules are the molecule basis of genetic code. Unfortunately, no such rules have been discovered for proteins.…

Biomolecules · Quantitative Biology 2017-02-28 Yuhong Wang , Junzhou Huang , Wei Li , Sheng Wang , Chuanfan Ding

Under supercoiling constraints, naked DNA, such as a large part of bacterial DNA, folds into braided structures called plectonemes. The double-helix can also undergo local structural transitions, leading to the formation of denaturation…

Biological Physics · Physics 2017-08-08 Thibaut Lepage , Ivan Junier

Twisting DNA under a constant applied force reveals a thermally activated transition into a state with a supercoiled structure known as a plectoneme. Using transition state theory, we predict the rate of this plectoneme nucleation to be of…

Biological Physics · Physics 2015-03-17 Bryan C. Daniels , James P. Sethna

Plectonemes are intertwined helically looped domains which form when a DNA molecule is supercoiled, i.e. over- or under-wounded. They are ubiquitous in cellular DNA and their physical properties have attracted significant interest both from…

Soft Condensed Matter · Physics 2022-09-14 Enrico Skoruppa , Enrico Carlon

Presented here, is a technical manuscript that may form the basis of later published work. In it, we develop a statistical mechanical model to describe a closed loop plectoneme, applicable for when the closed loop is sufficiently…

Soft Condensed Matter · Physics 2016-09-09 Dominic J. O'. Lee

A new set of DNA base-nucleic acid codes and their hypercomplex number representation have been introduced for taking the probability of each nucleotide into full account. A new scoring system has been proposed to suit the hypercomplex…

Other Quantitative Biology · Quantitative Biology 2014-03-12 Jian-Jun Shu , Li Shan Ouw

Fabrication of DNA nanostructures primarily follows two fundamental rules. First, DNA oligonucleotides mutually combine by Watson-Crick base pairing rules between complementary base sequences. Second, the geometrical compatibility of the…

Mesoscale and Nanoscale Physics · Physics 2011-06-01 Jihoon Shin , Junghoon Kim , Rashid Amin , Seungjae Kim , Young Hun Kwon , Sung Ha Park

A weak chord index $Ind'$ is constructed for self crossing points of virtual links. Then a new writhe polynomial $W$ of virtual links is defined by using $Ind'$. $W$ is a generalization of writhe polynomial defined in [6]. Based on $W$,…

Geometric Topology · Mathematics 2018-12-14 Mengjian Xu

DNA supercoiling plays an important role on a biological point of view. One of its consequences at the supra-molecular level is the formation of DNA superhelices named plectonemes. Normally separated by a distance on the order of 10 nm, the…

Soft Condensed Matter · Physics 2018-03-28 Céline Barde , Nicolas Destainville , Manoel Manghi

It is known that the writhe calculated from any reduced alternating link diagram of the same (alternating) link has the same value. That is, it is a link invariant if we restrict ourselves to reduced alternating link diagrams. This is due…

Geometric Topology · Mathematics 2020-09-29 Yuanan Diao , Van Pham

While slowly turning the ends of a single molecule of DNA at constant applied force, a discontinuity was recently observed at the supercoiling transition, when a small plectoneme is suddenly formed. This can be understood as an abrupt…

Biomolecules · Quantitative Biology 2011-03-15 Bryan C. Daniels , Scott Forth , Maxim Y. Sheinin , Michelle D. Wang , James P. Sethna
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