Related papers: Far-field fluorescence microscopy beyond the diffr…
A widely tested approach to overcoming the diffraction limit in microscopy without disturbing the sample relies on substituting widefield sample illumination with a structured light beam. This gives rise to confocal, image-scanning and…
Phase Fresnel lenses have the same imaging properties as zone plates, but with the possibility of concentrating all of the incident power into the primary focus, increasing the maximum theoretical efficiency from 11% to close to 100%. For…
Diffraction-limited imaging in epi-fluorescence microscopy remains a challenge when sample aberrations are present or when the region of interest rests deep within an inhomogeneous medium. Adaptive optics is an attractive solution albeit…
FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…
To overcome the resolution limit of conventional optics, near field imaging techniques using a negative index flat lens (NIFL) have been previously developed that amplify the evanescent components of the incident field. Here, a technique is…
The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…
We demonstrate that a deep neural network can significantly improve optical microscopy, enhancing its spatial resolution over a large field-of-view and depth-of-field. After its training, the only input to this network is an image acquired…
Optical scattering presents a major obstacle to high resolution imaging in biological tissue and other turbid media. Conventional photoacoustic imaging can partially overcome this obstacle, enabling imaging of optical absorption in the…
Hyperspectroscopy is a new method of surface image taking, providing simultaneously high position and spectral resolutions which allow one to make some conclusions about chemical compositions of the surfaces. We are now studying…
Perfect lensing using negative refractive index materials and radiationless electromagnetic interference both provide extreme subwavelength focusing by "amplifying" evanescent wave components that are usually lost. This paper provides a…
Through digital imaging, microscopy has evolved from primarily being a means for visual observation of life at the micro- and nano-scale, to a quantitative tool with ever-increasing resolution and throughput. Artificial intelligence, deep…
This paper presents a comprehensive numerical analysis of super-resolution imaging using dielectric microparticles, employing the Finite-Difference Time-Domain (FDTD) method to elucidate the mechanisms that enable resolution enhancements…
Visualization in the virtual image formed by dielectric microparticles has been shown to enable the distinction of objects that remain indistinguishable under direct observation. We perform the resolution analysis based on a full…
We report both sub-diffraction-limited quantum metrology and quantum enhanced spatial resolution for the first time in a biological context. Nanoparticles are tracked with quantum correlated light as they diffuse through an extended region…
Mid-infrared hyperspectral imaging has become an indispensable tool to spatially resolve chemical information in a wide variety of samples. However, acquiring three-dimensional data cubes is typically time-consuming due to the limited speed…
We introduce a full field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering.…
Compared to imaging in the visible and near-infrared regions below 900 nm, imaging in the second near-infrared window (NIR-II, 1000-1700 nm) is a promising method for deep-tissue high-resolution optical imaging in vivo mainly due to the…
Light field (LF) image super-resolution (SR) is a challenging problem due to its inherent ill-posed nature, where a single low-resolution (LR) input LF image can correspond to multiple potential super-resolved outcomes. Despite this…
Fluorescence microscopy is widely used for the study of biological specimens. Deconvolution can significantly improve the resolution and contrast of images produced using fluorescence microscopy; in particular, Bayesian-based methods have…
Converting the medium infrared field to the visible band is an effective image detection method. We propose a comprehensive theory of image up-conversion under continuous optical pumping, and discuss the relationship between the…