Related papers: Far-field fluorescence microscopy beyond the diffr…
A near-field microwave imaging system attempts to reveal the presence of an object and/or an electrical property distribution by measuring the scattered field from many positions surrounding the object. Over the past few decades, both the…
In recent years, a great deal of emphasis has been placed on achieving the diffraction limit with large aperture telescopes. For a well matched focal-plane instrument, the diffraction limit provides the highest possible angular resolution…
Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation,…
We demonstrate sub-wavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fuorescence, wherein only a fluorophore next to the…
Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent…
This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…
A variety of recent imaging techniques are able to beat the diffraction limit in fluorescence microcopy by activating and localizing subsets of the fluorescent molecules in the specimen, and repeating this process until all of the molecules…
Diffusion models have shown great results in image generation and in image editing. However, current approaches are limited to low resolutions due to the computational cost of training diffusion models for high-resolution generation. We…
Owing to its capacity for unique (bio)-chemical specificity, microscopy withmid-IR illumination holds tremendous promise for a wide range of biomedical and industrial applications. The primary limitation, however, remains detection; with…
Fourier back plane (FBP) imaging technique has been widely used in the frontier research of nanophotonics. In this paper, based on the diffraction theory and wave front transformation principle, the FBP imaging basic principle, the setup…
While Fourier ptychography (FP) offers super-resolution for macroscopic imaging, its real-world application is severely hampered by atmospheric turbulence, a challenge largely unaddressed in existing macroscopic FP research operating under…
There has been a consistent growth in research involving imaging of microvasculature over the past few decades. By 2008, publications mentioning the microcirculation had grown more than 2000 per annum. Many techniques have been demonstrated…
Mid-infrared photo-induced force microscopy (IR-PiFM/PiF-IR) enables high-resolution chemical imaging of surfaces with lateral resolution less than 5 nm. Here are some answers to questions about the physical background, practical handling…
Super-resolution imaging refers to imaging techniques that surpass the Rayleigh resolution limit. One standard way to achieve super-resolution is by structuring the phase of the field illuminating the object. Although super-resolution…
Diffractive/refractive optics, such as Phase Fresnel Lenses (PFL's), offer the potential to achieve excellent imaging performance in the x-ray and gamma-ray photon regimes. In principle, the angular resolution obtained with these devices…
We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and…
Ptychography involves a sample being illuminated by a coherent, localised probe of illumination. When the probe interacts with the sample, the light is diffracted and a diffraction pattern is detected. Then the probe or sample is shifted…
High-resolution imaging in the terahertz (THz) spectral range remains fundamentally constrained by the limited numerical apertures of currently existing state-of-the-art imagers, which restricts its applicability across many fields, such as…
Currently, to further improve visual enjoyment, Ultra-High-Definition (UHD) images are catching wide attention. Here, UHD images are usually referred to as having a resolution greater than or equal to $3840 \times 2160$. However, since the…
Medical image segmentation is crucial for accurate clinical diagnoses, yet it faces challenges such as low contrast between lesions and normal tissues, unclear boundaries, and high variability across patients. Deep learning has improved…