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Fourier imaging is an indirect imaging method which records the diffraction pattern of the object scene coherently in the focal plane of the imaging system and reconstructs the image using computational resources. The spatial resolution,…

Optics · Physics 2023-06-05 Hui Yuan , Alvydas Lisauskas , Mark D. Thomson , Hartmut G. Roskos

Second harmonic generation microscopy (SHG) is a powerful imaging modality which has found applications in investigating both biological and synthetic nanostructures. Like all optical microscopy techniques, the resolution of SHG is limited…

Photonic sensors have many applications in a range of physical settings, from measuring mechanical pressure in manufacturing to detecting protein concentration in biomedical samples. A variety of sensing approaches exist, and plasmonic…

Interferometers are widely used in imaging technologies to achieve enhanced spatial resolution, but require that the incoming photons be indistinguishable. In previous work, we built and analyzed color erasure detectors which expand the…

Diffractive X-ray telescopes using zone plates, phase Fresnel lenses, or related optical elements have the potential to provide astronomers with true imaging capability with resolution several orders of magnitude better than available in…

Instrumentation and Methods for Astrophysics · Physics 2010-09-14 Gerald K. Skinner

We demonstrate a new 'microsphere nanoscope' that uses ordinary SiO2 microspheres as superlenses to create a virtual image of the object in near field. The magnified virtual image greatly overcomes the diffraction limit. We are able to…

Optics · Physics 2012-03-21 Zengbo Wang , Wei Guo , Lin Li , Zhu Liu , Boris Luk'yanchuk , Zaichun Chen , Minghui Hong

The manifestation of the wave nature of light through diffraction imposes limits on the resolution of optical imaging. For over a century, the Abbe-Rayleigh criterion has been utilized to assess the spatial resolution limits of optical…

Conventional microscope objective lenses are diffraction limited, which means that they cannot resolve features smaller than half the illumination wavelength. Under white light illumination, such resolution limit is about 250-300 nm for an…

Instrumentation and Detectors · Physics 2021-08-26 Bing Yan , Zengbo Wang , Alan Parker , Yukun Lai , John Thomas , Liyang Yue , James Monks

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Light-field imaging is an emerging paradigm in biomedical optics, offering the unique ability to capture volumetric information in a single snapshot by encoding both the spatial and angular components of light. Unlike conventional…

Optics · Physics 2025-09-30 Ruixuan Zhao , Xuanwen Hua , Woongjae Baek , Zhaoqiang Wang , Shu Jia , Liang Gao

Our visual perception of our surroundings is ultimately limited by the diffraction limit, which stipulates that optical information smaller than roughly half the illumination wavelength is not retrievable. Over the past decades, many…

Fluorescence microscopy, widely used in the study of living cells, tissues, and organisms, often faces the challenge of photodamage. This is primarily caused by the interaction between light and biochemical components during the imaging…

Quantitative Methods · Quantitative Biology 2024-04-01 Estibaliz Gómez-de-Mariscal , Mario Del Rosario , Joanna W Pylvänäinen , Guillaume Jacquemet , Ricardo Henriques

The ability to resolve detail in the object that is being imaged, named by resolution, is the core parameter of an imaging system. Super-resolution is a class of techniques that can enhance the resolution of an imaging system and even…

Data Structures and Algorithms · Computer Science 2022-10-13 Yaonan Jin , Daogao Liu , Zhao Song

One of the fundamental limits of classical optical microscopy is the diffraction limit of optical resolution. It results from the finite bandwidth of the optical transfer function (or OTF) of an optical microscope, which restricts the…

Optics · Physics 2022-03-11 Sjoerd Stallinga , Niels Radmacher , Antoine Delon , Jörg Enderlein

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

In deep tissue photoacoustic imaging the spatial resolution is inherently limited by the acoustic wavelength. Recently, it was demonstrated that it is possible to surpass the acoustic diffraction limit by analyzing fluctuations in a set of…

We report the experimental demonstration of deeply subwavelength far-field optical imaging of unlabelled samples with resolution better than $\lambda/20$. We beat the ~$\lambda$/2 diffraction limit of conventional optical microscopy several…

Optics · Physics 2020-01-07 Tanchao Pu , Jun-Yu Ou , Nikitas Papasimakis , Nikolay I. Zheludev

Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) to segment the Fourier Plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional(3D)…

Optics · Physics 2022-08-29 Haixin Huang , Haoyuan Qiu , Hanzhe Wu , Yihong Ji , Heng Li , Bin Yu , Danni Chen , Junle Qu

The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware…

We report the application of fifth order nonlinear optical processes for multiphoton microscopy, including fifth harmonic and 5-photon excitation fluorescence. This novel imaging modality has been characterized with spectral and power…

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