Fifth Harmonic and Five-Photon Excitation Fluorescence Multiphoton Microscopy
Optics
2025-06-30 v2
Abstract
We report the application of fifth order nonlinear optical processes for multiphoton microscopy, including fifth harmonic and 5-photon excitation fluorescence. This novel imaging modality has been characterized with spectral and power dependence measurements. The advantages of fifth harmonic and 5-photon excitation fluorescence imaging are higher resolution and use of longer wavelength excitation sources, which may allow deeper penetration depth, while still exciting near-UV to blue fluorophores. This imaging modality may find interesting applications in biological research, materials characterization, geologic studies, and semiconductor manufacturing.
Cite
@article{arxiv.2504.20309,
title = {Fifth Harmonic and Five-Photon Excitation Fluorescence Multiphoton Microscopy},
author = {Joshua H. Magnus and Lam T. Nguyen and Elana G. Alevy and Travis W. Sawyer and Samuel D. Crossley and Khanh Kieu},
journal= {arXiv preprint arXiv:2504.20309},
year = {2025}
}