Simultaneous Multi-Harmonic Imaging of Nanoparticles in Tissues for Increased Selectivity
Optics
2015-11-05 v2
Abstract
We investigate the use of Bismuth Ferrite (BFO) nanoparticles for tumor tissue labelling in combination with infrared multi-photon excitation at 1250 nm. We report the efficient and simultaneous generation of second and third harmonic by the nanoparticles. On this basis, we set up a novel imaging protocol based on the co-localization of the two harmonic signals and demonstrate its benefits in terms of increased selectivity against endogenous background sources in tissue samples. Finally, we discuss the use of BFO nanoparticles as mapping reference structures for correlative light-electron microscopy.
Keywords
Cite
@article{arxiv.1410.7969,
title = {Simultaneous Multi-Harmonic Imaging of Nanoparticles in Tissues for Increased Selectivity},
author = {Andrii Rogov and Marie Irondelle and Fernanda Ramos-Gomes and Julia Bode and Davide Staedler and Solène Passemard and Sébastien Courvoisier and Yasuaki Yamamoto and François Waharte and Daniel Ciepielewski and Philippe Rideau and Sandrine Gerber-Lemaire and Frauke Alves and Jean Salamero and Luigi Bonacina and Jean-Pierre Wolf},
journal= {arXiv preprint arXiv:1410.7969},
year = {2015}
}
Comments
19 pages, 6 figures