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Using a microfluidic trap, we study the behavior of individual phospholipid vesicles in contact with fatty acids. We show that spontaneous fatty acids insertion inside the bilayer is controlled by the vesicle size, osmotic pressure…

亚细胞过程 · 定量生物学 2016-12-14 Julien Dervaux , Vincent Noireaux , Albert Libchaber

The interaction of two types of vesicle systems was investigated: micrometer-sized, giant unilamellar vesicles (GUVs) formed from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and submicrometer-sized, large unilamellar vesicles…

生物物理 · 物理学 2009-05-26 Primoz Peterlin , Vesna Arrigler , Ksenija Kogej , Sasa Svetina , Peter Walde

The hydration process of multi-stacked phospholipid bilayers to form giant vesicles was investigated by time-resolved small angle X-ray scattering. The hydration of lipids in the liquid-crystalline phase was found to proceed in two stages.…

化学物理 · 物理学 2007-05-23 M. Hishida , H. Seto , N. L. Yamada , K. Yoshikawa

A coarse-grained model developed by Marrink et al. [J. Phys. Chem. B 111, 7812 (2007)] is applied to investigate vesiculation of lipid [dipalmitoylphosphatidylcholine (DPPC)] droplets in water. Three kinds of morphologies of micelles are…

软凝聚态物质 · 物理学 2009-11-13 Zilu Wang , Xuehao He

We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a…

软凝聚态物质 · 物理学 2022-07-14 Pierre Tapie , Alexis M. Prevost , Lorraine Montel , Léa-Laetitia Pontani , Elie Wandersman

The study of interactions between biomimetic membranes and micron-sized particles is crucial for understanding various biological processes. Here, we control microparticle spontaneous engulfment by giant lipid vesicles by tuning particle…

软凝聚态物质 · 物理学 2025-01-22 Clément Marque , Gaetano D'Avino , Domenico Larobina , Aude Michel , Ali Abou-Hassan , Antonio Stocco

Entry of micro- or nano-sized objects into cells or vesicles made of lipid membranes occur in many processes such as entry of viruses in host cells, microplastics pollution, drug delivery or biomedical imaging. Here, we investigated the…

软凝聚态物质 · 物理学 2023-05-24 Florent Fessler , Vaibhav Sharma , Pierre Muller , Antonio Stocco

This paper demonstrates that disposable polyester-toner microfluidic devices are suitable to produce either water-in-oil (W/O) or oil-in-water (O/W) droplets without using any surface treatment of the microchannels walls. Highly…

We investigate the influence of a non-ionic surfactant like polymer on phospholipid vesicles. Our results from cryogenic transmission electron microscopy (cryo-TEM), dynamic light scattering (DLS), small angle neutron and X-ray scattering…

Lipid bilayer membranes are the fundamental biological barriers that permit life. The bilayer dynamics largely participates in orchestrating cellular workings and is characterized by substantial stability together with extreme plasticity…

软凝聚态物质 · 物理学 2022-12-05 Matteo Bottacchiari , Mirko Gallo , Marco Bussoletti , Carlo Massimo Casciola

Relatively short peptides, such as toxins and antimicrobial-peptides, are known to insert themselves into cell membranes. On the basis of simple bead-spring models for the membrane lipids, the peptide, and water, detailed processes of the…

软凝聚态物质 · 物理学 2007-05-23 Mitsuharu Okazaki , Tomoki Watanabe , Naohito Urakami , Takashi Yamamoto

Vesicle fusion is an important process underlying cell division, transport, and membrane trafficking. In phospholipid systems, a range of fusogens including divalent cations and depletants have been shown to induce adhesion, hemifusion, and…

软凝聚态物质 · 物理学 2023-05-03 Ziyan Fan , Yaam Deckel , Lauren A. Lowe , Daniel W. K. Loo , Tetsuya Yomo , Jack W. Szostak , Collin Nisler , Anna Wang

Droplet formation in a system of two or more immiscible fluids is a celebrated topic of research in the fluid mechanics community. In this work, we propose an innovative phenomenon where oil when injected drop-wise into a pool of water…

流体动力学 · 物理学 2013-11-26 Saurabh Nath , Anish Mukherjee , Souvick Chatterjee

The bursting of bubbles at an air/liquid interface is a familiar occurrence important to foam stability, cell cultures in bioreactors and mass transfer between the sea and atmosphere. Here we document the hitherto unreported formation and…

We report results concerning the destabilisation of supported phospholipid bilayers in a well-defined geometry. When heating up supported phospholipid membranes deposited on highly hydrophilic glass slides from room temperature (i.e. with…

软凝聚态物质 · 物理学 2007-05-23 S. Lecuyer , T. Charitat

Phospholipid bilayers are a major component of the cell membrane that is in contact with physiological electrolyte solutions including salt ions. The effect of salt on the phospholipid bilayer mechanics is an active research area due to its…

软凝聚态物质 · 物理学 2023-10-16 Yaoqi Huang , Vineeth Chandran Suja , Layaa Amirthalingam , Gerald G. Fuller

Microfluidic techniques have been extensively developed to realize micro-total analysis systems in a small chip. For microanalysis, electro-magnetic forces have generally been utilized for the trapping of objects, but hydrodynamics has been…

流体动力学 · 物理学 2021-04-22 Takuya Ohmura , Masatoshi Ichikawa , Ken-ichiro Kamei , Yusuke T. Maeda

Droplet interface bilayers are a convenient model system to study the physio-chemical properties of phospholipid bilayers, the major component of the cell membrane. The mechanical response of these bilayers to various external mechanical…

生物物理 · 物理学 2021-02-08 Yaoqi Huang , Vineeth Chandran Suja , Javier Tajuelo , Gerald G. Fuller

We report that a cell-sized water droplet (CWD) with a diameter of several tens of microns can serve as a micro-biochemical reactor. Since the droplet inner surface is covered by a phospholipid layer, it provides an environment similar to…

生物物理 · 物理学 2007-05-23 Masahiko Hase , Ayako Yamada , Tsutomu Hamada , Kenichi Yoshikawa

The biogenesis of lipid droplets (LD) in the yeast Saccharomyces cerevisiae was theoretically investigated on basis of a biophysical model. In accordance with the prevailing model of LD formation, we assumed that neutral lipids oil-out…

亚细胞过程 · 定量生物学 2009-10-30 J. Zanghellini , F. Wodlei , H. -H. von Gruenberg
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