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We introduce a comprehensive framework for modeling single cell transcriptomic responses to perturbations, aimed at standardizing benchmarking in this rapidly evolving field. Our approach includes a modular and user-friendly model…

Xenium, a new spatial transcriptomics platform, enables subcellular-resolution profiling of complex tumor tissues. Despite the rich morphological information in histology images, extracting robust cell-level features and integrating them…

计算机视觉与模式识别 · 计算机科学 2025-08-15 Paul H. Acosta , Pingjun Chen , Simon P. Castillo , Maria Esther Salvatierra , Yinyin Yuan , Xiaoxi Pan

Single-cell multi-omics (scMulti-omics) refers to the paired multimodal data, such as Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq), where the regulation of each cell was measured from different modalities, i.e.…

机器学习 · 计算机科学 2024-10-18 Dian Meng , Bohao Xing , Xinlei Huang , Yanran Liu , Yijun Zhou , Yongjun xiao , Zitong Yu , Xubin Zheng

Influenced by breakthroughs in LLMs, single-cell foundation models are emerging. While these models show successful performance in cell type clustering, phenotype classification, and gene perturbation response prediction, it remains to be…

Advances in single-cell sequencing have enabled high-resolution profiling of diverse molecular modalities, while integrating unpaired multi-omics single-cell data remains challenging. Existing approaches either rely on pair information or…

定量方法 · 定量生物学 2026-01-21 Jianle Sun , Chaoqi Liang , Ran Wei , Peng Zheng , Lei Bai , Wanli Ouyang , Hongliang Yan , Peng Ye

Phenotypic drug discovery has attracted widespread attention because of its potential to identify bioactive molecules. Transcriptomic profiling provides a comprehensive reflection of phenotypic changes in cellular responses to external…

机器学习 · 计算机科学 2025-01-16 Hui Liu , Shikai Jin

Spatial transcriptomics (ST) enables transcriptome-wide profiling while preserving the spatial context of tissues, offering unprecedented opportunities to study tissue organization and cell-cell interactions in situ. Despite recent…

计算机视觉与模式识别 · 计算机科学 2026-01-21 Wei Wang , Quoc-Toan Ly , Chong Yu , Jun Bai

Cellular identity and function are linked to both their intrinsic genomic makeup and extrinsic spatial context within the tissue microenvironment. Spatial transcriptomics (ST) offers an unprecedented opportunity to study this, providing in…

机器学习 · 计算机科学 2026-02-16 Rui Yan , Xiaohan Xing , Xun Wang , Zixia Zhou , Md Tauhidul Islam , Lei Xing

Single-cell RNA sequencing (scRNA-seq), especially temporally resolved datasets, enables genome-wide profiling of gene expression dynamics at single-cell resolution across discrete time points. However, current technologies provide only…

基因组学 · 定量生物学 2025-11-19 Yue Ling , Peiqi Zhang , Zhenyi Zhang , Peijie Zhou

Transcriptome foundation models TFMs hold great promises of deciphering the transcriptomic language that dictate diverse cell functions by self-supervised learning on large-scale single-cell gene expression data, and ultimately unraveling…

机器学习 · 计算机科学 2025-03-03 Xinyu Yuan , Zhihao Zhan , Zuobai Zhang , Manqi Zhou , Jianan Zhao , Boyu Han , Yue Li , Jian Tang

Predicting cellular responses to genetic perturbations represents a fundamental challenge in systems biology, critical for advancing therapeutic discovery and virtual cell modeling. While large language models (LLMs) show promise for…

Celcomen leverages a mathematical causality framework to disentangle intra- and inter- cellular gene regulation programs in spatial transcriptomics and single-cell data through a generative graph neural network. It can learn gene-gene…

The integration of single-cell proteomic data is often hindered by the fragmented nature of targeted antibody panels. To address this limitation, we introduce scpFormer, a transformer-based foundation model designed for single-cell…

定量方法 · 定量生物学 2026-04-23 Qifeng Zhou , Lei Yu , Yuzhi Guo , Yuwei Miao , Hehuan Ma , Wenliang Zhong , Lin Xu , Junzhou Huang

Recent advances in single cell sequencing and multi-omics techniques have significantly improved our understanding of biological phenomena and our capacity to model them. Despite combined capture of data modalities showing similar progress,…

Microscopy-based phenotypic profiling is scalable for drug discovery but lacks the mechanistic depth of transcriptomics, which remains costly and scarce. Existing multimodal approaches either use images to support other modalities or…

计算机视觉与模式识别 · 计算机科学 2026-04-28 Jiayuan Chen , Ruoqi Liu , Zishan Gu , Ping Zhang

Understanding the relationships among genes, compounds, and their interactions in living organisms remains limited due to technological constraints and the complexity of biological data. Deep learning has shown promise in exploring these…

Pathology foundation models learn morphological representations through self-supervised pretraining on large-scale whole-slide images, yet they do not explicitly capture the underlying molecular state of the tissue. Spatial transcriptomics…

计算机视觉与模式识别 · 计算机科学 2026-03-10 Minsoo Lee , Jonghyun Kim , Juseung Yun , Sunwoo Yu , Jongseong Jang

Digital Pathology is a cornerstone in the diagnosis and treatment of diseases. A key task in this field is the identification and segmentation of cells in hematoxylin and eosin-stained images. Existing methods for cell segmentation often…

计算机视觉与模式识别 · 计算机科学 2025-01-10 Fabian Hörst , Moritz Rempe , Helmut Becker , Lukas Heine , Julius Keyl , Jens Kleesiek

The development of single-cell and spatial transcriptomics has revolutionized our capacity to investigate cellular properties, functions, and interactions in both cellular and spatial contexts. However, the analysis of single-cell and…

基因组学 · 定量生物学 2024-12-09 Shuang Ge , Shuqing Sun , Huan Xu , Qiang Cheng , Zhixiang Ren

A central goal in systems biology and drug discovery is to predict the transcriptional response of cells to perturbations. This task is challenging due to the noisy and sparse nature of single-cell measurements, as well as the fact that…

定量方法 · 定量生物学 2026-02-10 Chenglei Yu , Chuanrui Wang , Bangyan Liao , Tailin Wu
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