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Polarized light microscopy provides unique opportunities for analyzing the molecular order in man-made and natural materials, including biological structures inside living cells, tissues, and whole organisms. 20 years ago, the LC-PolScope…

光学 · 物理学 2015-06-16 Shalin B. Mehta , Michael Shribak , Rudolf Oldenbourg

Polarization imaging is a technique that creates a pixel map of the polarization state in a scene. Although invisible to the human eye, polarization can assist various sensing and computer vision tasks. Existing polarization cameras use…

图像与视频处理 · 电气工程与系统科学 2026-03-19 Noa Kraicer , Shay Elmalem , Erez Yosef , Hani Barhum , Raja Giryes

Optical metasurfaces have been recently explored as ultrathin analog image differentiators. By tailoring the momentum transfer function, they can perform efficient Fourier-filtering - and thus potentially any linear mathematical operation -…

Three-dimensional (3D) subcellular imaging is essential for biomedical research, but the diffraction limit of optical microscopy compromises axial resolution, hindering accurate 3D structural analysis. This challenge is particularly…

Polarisation imaging is used to distinguish objects and surface characteristics that are otherwise not visible with black-and-white or colour imaging. Full-Stokes polarisation imaging allows complex image processing like water glint…

As a basic optical element, circular polarizer plays significant roles in signal transmission, measurements and life science microscopy. Three-dimensional (3D) chiral structures have been thought to be necessary to realize circular…

光学 · 物理学 2014-10-13 Weimin Ye , Xiaodong Yuan , Chucai Guo , Jianfa Zhang , Biao Yang , Ken Liu , Zhihong Zhu , Chun Zeng

We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…

光学 · 物理学 2018-01-04 Seungwoo Shin , Doyeon Kim , Kyoohyun Kim , Yongkeun Park

3D imaging of specular surfaces remains challenging in real-world scenarios, such as in-line inspection or hand-held scanning, requiring fast and accurate measurement of complex geometries. Optical metrology techniques such as deflectometry…

计算机视觉与模式识别 · 计算机科学 2026-04-23 Jiazhang Wang , Hyelim Yang , Tianyi Wang , Florian Willomitzer

Matrix-Assisted Laser Desorption/Ionisation Mass Spectrometry Imaging (MSI) is a powerful technique for spatially resolved molecular profiling and cancer biomarker discovery. Recent advances, including a novel multiomics workflow, enable…

统计方法学 · 统计学 2025-09-03 Francesco Denti , Cecilia Balocchi , Vanna Denti , Giulia Capitoli

Epithelial cells form diverse structures from squamous spherical organoids to densely packed pseudostratified tissues. Quantification of cellular properties in these contexts requires high-resolution deep imaging and computational…

定量方法 · 定量生物学 2025-02-03 G. Paci , P. Vicente-Munuera , I. Fernandez-Mosquera , A. Miranda , K. Lau , Q. Zhang , R. Barrientos , Y. Mao

Three-dimensional (3D) imaging of the subcellular organisation and morphology of cells and tissues is essential for understanding biological function. Although staining is the most widely used approach for visualising biological samples…

Hierarchical biomaterials embody nature's intricate design principles, offering advanced functionalities through the complex, multi-level organization of their molecular and nanosized building blocks. However, the comprehensive…

介观与纳米尺度物理 · 物理学 2024-07-10 Matteo Busi , Elisabetta Nocerino , Agnes Åhl , Lennart Bergström , Markus Strobl

The real-time, in-line analysis of light polarization is critical in optical communication networks, which suffers from the complex systems with numerous bulky opto-electro-mechanical elements tandemly arranged along optical path. Here, we…

A new scheme based on (i) upcoming brilliant X-ray Free Electron Laser (FEL) sources, (ii) novel energy and angular dispersive, large-area electron imagers and (iii) the well-known photoelectron holography is elaborated that provides…

原子物理 · 物理学 2015-05-14 F. Krasniqi , B. Najjari , L. Strüder , D. Rolles , A. Voitkiv , J. Ullrich

Comprehensive assessment of the various aspects of the brain's microstructure requires the use of complementary imaging techniques. This includes measuring the spatial distribution of cell bodies (cytoarchitecture) and nerve fibers…

Label-free characterization of biological specimens seeks to supplement existing imaging techniques and avoid the need for contrast agents that can disturb the native state of living samples. Conventional label-free optical imaging…

Porous poly(ionic liquid)s (PILs) recently have been actively serving as a multifunctional, interdisciplinary materials platform in quite a few research areas, including separation, catalysis, actuator, sensor, and energy storage, just to…

化学物理 · 物理学 2019-02-07 Ming-Jie Yin , Qiang Zhao , Jushuai Wu , Karoline Seefeldt , Jiayin Yuan

Fast, volumetric imaging that integrates multiple imaging modalities is essential for probing dynamic, heterogeneous soft and biological matter. Here, we present the M3Scope, a simple yet versatile multiplane microscope that extends…

Recent advances in deep learning-based image reconstruction techniques have led to significant progress in phase retrieval using digital in-line holographic microscopy (DIHM). However, existing deep learning-based phase retrieval methods…

计算机视觉与模式识别 · 计算机科学 2024-10-01 Jihwan Kim , Youngdo Kim , Hyo Seung Lee , Eunseok Seo , Sang Joon Lee

We perform a theoretical and numerical study of the optical properties of both direct and inverse three-dimensional (3D) chiral woodpile structures, and a corresponding chiral Bragg stack. We compute transmission spectra in the helical…