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Accurate intraoperative assessment of glioma infiltration is essential for maximizing tumor resection while preserving functional brain tissue. Fluorescence lifetime imaging (FLIm) offers real-time, label-free biochemical contrast, but its…

计算机视觉与模式识别 · 计算机科学 2026-04-30 Silvia Noble Anbunesan , Mohamed Abul Hassan , Jinyi Qi , Lisanne Kraft , Han Sung Lee , Orin Bloch , Laura Marcu

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

计算机视觉与模式识别 · 计算机科学 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis,…

图像与视频处理 · 电气工程与系统科学 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Cara Ravasio , Scott S. Howard

Fluorescence lifetime imaging microscopy (FLIM) systems are limited by their slow processing speed, low signal-to-noise ratio (SNR), and expensive and challenging hardware setups. In this work, we demonstrate applying a denoising…

图像与视频处理 · 电气工程与系统科学 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Takashi Hato , Pierre C. Dagher , Evan L. Nichols , Cody J. Smith , Kenneth W. Dunn , Scott Howard

Fluorescence Lifetime Imaging (FLI) is a critical molecular imaging modality that provides unique information about the tissue microenvironment, which is invaluable for biomedical applications. FLI operates by acquiring and analyzing photon…

图像与视频处理 · 电气工程与系统科学 2024-12-06 Ismail Erbas , Vikas Pandey , Navid Ibtehaj Nizam , Nanxue Yuan , Amit Verma , Margarida Barosso , Xavier Intes

The research community has witnessed the powerful potential of self-supervised Masked Image Modeling (MIM), which enables the models capable of learning visual representation from unlabeled data. In this paper, to incorporate both the…

计算机视觉与模式识别 · 计算机科学 2023-12-01 Wenxuan Wang , Jing Wang , Chen Chen , Jianbo Jiao , Yuanxiu Cai , Shanshan Song , Jiangyun Li

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

图像与视频处理 · 电气工程与系统科学 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

We present a fast and accurate analytical method for fluorescence lifetime imaging microscopy (FLIM) using the extreme learning machine (ELM). We used extensive metrics to evaluate ELM and existing algorithms. First, we compared these…

生物物理 · 物理学 2022-03-28 Zhenya Zang , Dong Xiao , Quan Wang , Zinuo Li , Wujun Xie , Yu Chen , David Day Uei Li

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

医学物理 · 物理学 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

Many healthcare sensing applications utilize multimodal time-series data from sensors embedded in mobile and wearable devices. Federated Learning (FL), with its privacy-preserving advantages, is particularly well-suited for health…

机器学习 · 计算机科学 2024-11-28 Adiba Orzikulova , Jaehyun Kwak , Jaemin Shin , Sung-Ju Lee

Salient Object Detection (SOD) with deep learning often requires substantial computational resources and large annotated datasets, making it impractical for resource-constrained applications. Lightweight models address computational demands…

计算机视觉与模式识别 · 计算机科学 2025-04-16 Leonardo M. Joao , Jancarlo F. Gomes , Silvio J. F. Guimaraes , Ewa Kijak , Alexandre X. Falcao

Cryo Focused Ion-Beam Scanning Electron Microscopy (cryo FIB-SEM) enables three-dimensional and nanoscale imaging of biological specimens via a slice and view mechanism. The FIB-SEM experiments are, however, limited by a slow (typically,…

We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and…

定量方法 · 定量生物学 2009-11-10 R. V. Krishnan , H. Saitoh , H. Terada , V. E. Centonze , B. Herman

To date, endovascular surgeries are performed using the golden standard of Fluoroscopy, which uses ionising radiation to visualise catheters and vasculature. Prolonged Fluoroscopic exposure is harmful for the patient and the clinician, and…

图像与视频处理 · 电气工程与系统科学 2023-09-27 Alex Ranne , Yordanka Velikova , Nassir Navab , Ferdinando Rodriguez y Baena

Multimodal and multi-information microscopy techniques such as Fluorescence Lifetime Imaging Microscopy (FLIM) extend the informational channels beyond intensity-based fluorescence microscopy but suffer from reduced image quality due to…

图像与视频处理 · 电气工程与系统科学 2025-04-15 Hao Chen , Julian Najera , Dagmawit Geresu , Meenal Datta , Cody Smith , Scott Howard

In this study, a radiomics approach was extended to optical fluorescence molecular imaging data for tissue classification, termed 'optomics'. Fluorescence molecular imaging is emerging for precise surgical guidance during head and neck…

In developing medical interventions using untethered milli- and microrobots, ensuring safety and effectiveness relies on robust methods for detection, real-time tracking, and precise localization within the body. However, the inherent…

机器人学 · 计算机科学 2024-09-16 Husnu Halid Alabay , Tuan-Anh Le , Hakan Ceylan

While recent advances in deep learning have significantly advanced the state of the art for vessel detection in color fundus (CF) images, the success for detecting vessels in fluorescein angiography (FA) has been stymied due to the lack of…

计算机视觉与模式识别 · 计算机科学 2020-05-12 Li Ding , Mohammad H. Bawany , Ajay E. Kuriyan , Rajeev S. Ramchandran , Charles C. Wykoff , Gaurav Sharma

Fiber orientation distribution (FOD) is an advanced diffusion MRI modeling technique that represents complex white matter fiber configurations, and a key step for subsequent brain tractography and connectome analysis. Its reliability and…

Purpose: Mechanical thrombectomy (MT) improves stroke outcomes, but is limited by a lack of local treatment access. Widespread distribution of reinforcement learning (RL)-based robotic systems can be used to alleviate this challenge through…

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