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Phase Measuring Deflectometry (PMD) acquires the two components of the local surface gradient via a sequence of two orthogonal sinusoidal fringe patterns that have to be displayed and captured separately. We will demonstrate that the…

光学 · 物理学 2014-04-24 Yuankun Liu , Evelyn Olesch , Zheng Yang , Gerd Häusler

F\"orster theory describes electronic exciton energy migration in molecular assemblies as an incoherent hopping process between donor and acceptor molecules. The rate is expressed in terms of the overlap integral between donor fluorescence…

化学物理 · 物理学 2018-10-17 Marco Schröter , Tõnu Pullerits , Oliver Kühn

Several past studies have described how absorption spectroscopy can be used to determine spatial temperature variations along the optical path by measuring the unique, nonlinear response to temperature of many molecular absorption…

应用物理 · 物理学 2021-08-18 Nathan Malarich , Greg Rieker

Single fluorescent molecules, behaving as ideal electric dipole emitters, are powerful nanoscopic probes of complex optical fields. Here, this property is exploited to precisely map the polarization and vectorial structure of tightly…

Photothermal spectroscopy and microscopy provides a route to measure the spectral and spatial properties of individual nanoscopic absorbers, independent from scattering, extinction, and emission. The approach relies upon use of two light…

光学 · 物理学 2021-04-12 Harrison J. Goldwyn , Stephan Link , David J. Masiello

We investigate the possibility of non-destructive electron diffraction imaging of a single molecule to determine its structure. The molecular specimen will be held on a free-standing sheet of graphene. Due to the high conductivity of…

化学物理 · 物理学 2009-11-13 E. E. Fill , F. Krausz , M. Raizen

Single-shot fluorescence imaging techniques have gained increasing interest in recent years due to their ability to rapidly capture complex biological data without the need for extensive scanning. In this letter, we introduce polarized…

光学 · 物理学 2025-01-31 Oumeng Zhang , Changhuei Yang

Time course measurement of single molecules on a cell surface provides detailed information on the dynamics of the molecules, which is otherwise inaccessible. To extract the quantitative information, single particle tracking (SPT) is…

定量方法 · 定量生物学 2016-01-06 Shunsuke Teraguchi , Yutaro Kumagai

The development of single-photon sources has been nothing but rapid in recent years, with quantum emitter-based systems showing especially impressive progress. In this article, we give an overview of the developments in single-photon…

量子物理 · 物理学 2026-05-14 Alexey Shkarin , Stephan Götzinger

Single-molecule fluorescence techniques are essential for investigating the molecular mechanisms in biological processes. However, achieving sub-millisecond temporal resolution to monitor fast molecular dynamics remains a significant…

光学 · 物理学 2023-03-02 Sunny Tiwari , Prithu Roy , Jean-Benoît Claude , Jérôme Wenger

We investigate theoretically a scheme for spectroscopy of electrons emitted by an on-demand single particle source. The total system, with an electron turnstile source and a single level quantum dot spectrometer, is implemented with edge…

介观与纳米尺度物理 · 物理学 2015-06-03 F. Battista , P. Samuelsson

Background: Windowed Fourier decompositions (WFD) are widely used in measuring stationary and non-stationary spectral phenomena and in describing pairwise relationships among multiple signals. Although a variety of WFDs see frequent…

定量方法 · 定量生物学 2019-01-30 Christopher K. Kovach , Phillip E. Gander

Optical spectrum analysis is the cornerstone of spectroscopic sensing, optical network performance monitoring, and hyperspectral imaging. While conventional high-performance spectrometers used to perform such analysis are often large…

应用物理 · 物理学 2018-03-19 Derek M. Kita , Brando Miranda , David Favela , David Bono , Jerome Michon , Hongtao Lin , Tian Gu , Juejun Hu

Interferometry has underpinned a century of discoveries, ranging from the disproval of the ether theory to the detection of gravitational waves, offering insights into wave dynamics with unrivalled precision through the measurement of phase…

Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…

生物大分子 · 定量生物学 2015-04-15 Mark Leake

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

Progress in electron-beam spectroscopies has recently enabled the study of optical excitations with combined space, energy and time resolution in the nanometer, millielectronvolt and femtosecond domain, thus providing unique access into…

光学 · 物理学 2019-11-13 Albert Polman , Mathieu Kociak , F. Javier García de Abajo

Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…

光学 · 物理学 2015-06-15 Eric D. Diebold , Brandon W. Buckley , Daniel R. Gossett , Bahram Jalali

We measure the AC-Stark shifts of the 5S{1/2}, F=2 to 5P{3/2}, F'=3 transitions of individual optically trapped 87Rb atoms using a non-destructive detection technique that allows us to measure the fluorescent signal of one-and-the-same atom…

原子物理 · 物理学 2015-06-15 Chung-Yu Shih , Michael S. Chapman

We demonstrate spectroscopy of incoherent light with sub-diffraction resolution. In a proof-of-principle experiment we analyze the spectrum of a pair of incoherent point-like sources whose separation is below the diffraction limit. The two…

光学 · 物理学 2025-03-24 Luigi Santamaria Amato , Fabrizio Sgobba , Deborah Pallotti , Cosmo Lupo