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Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds…

Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the process is laborious and time-consuming,…

Three-dimensional (3D) histopathology of unprocessed tissues has the potential to transform disease management by enabling volumetric characterization of tissue microarchitecture and in-vivo assessment. Back-illumination Interference…

计算机视觉与模式识别 · 计算机科学 2026-05-22 Anthony Song , Boyan Zhou , Mayank Golhar , Marisa Morakis , Alex Baras , Nicholas Durr

Label-free metabolic dynamics contrast is highly appealing but difficult to achieve in biomedical imaging. Interference offers a highly sensitive mechanism for capturing the metabolic dynamics of the subcellular scatterers. However,…

Confocal laser endomicroscopy (CLE) allow on-the-fly in vivo intraoperative imaging in a discreet field of view, especially for brain tumors, rather than extracting tissue for examination ex vivo with conventional light microscopy.…

Histopathological imaging is vital for cancer research and clinical practice, with multiplexed Immunofluorescence (MxIF) and Hematoxylin and Eosin (H&E) providing complementary insights. However, aligning different stains at the cell level…

图像与视频处理 · 电气工程与系统科学 2024-10-02 Jun Jiang , Raymond Moore , Brenna Novotny , Leo Liu , Zachary Fogarty , Ray Guo , Markovic Svetomir , Chen Wang

Optical coherence tomography (OCT) is a label-free, non-invasive 3D imaging tool widely used in both biological research and clinical diagnosis. Current OCT modalities can only visualize specimen tomography without chemical information.…

While optical coherence tomography (OCT) provides a resolution down to 1 micrometer it has difficulties to visualize cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast…

An organoid is a three-dimensional (3D) in vitro cell culture emulating human organs. We applied 3D dynamic optical coherence tomography (DOCT) to visualize the intratissue and intracellular activities of human induced pluripotent stem…

Computer-aided diagnosis (CAD) can be used as an important tool to aid and enhance pathologists' diagnostic decision-making. Deep learning techniques, such as convolutional neural networks (CNN) and fully convolutional networks (FCN), have…

图像与视频处理 · 电气工程与系统科学 2023-03-21 Dalí F. D. dos Santos , Paulo R. de Faria , Adriano M. Loyola , Sérgio V. Cardoso , Bruno A. N. Travençolo , Marcelo Z. do Nascimento

Retinal degenerative diseases lead to the blindness of millions of people around the world. In case of age-related macular degeneration (AMD), the atrophy of retinal pigment epithelium (RPE) precedes neural dystrophy. But as crucial as…

Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method…

光学 · 物理学 2025-09-11 Charlie Kersuzan , Anirban Jana , Amaury Badon

Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as an invaluable live label-free and non-invasive imaging modality able to image subcellular biological structures and their metabolic activity within complex 3D…

Virtual staining streamlines traditional staining procedures by digitally generating stained images from unstained or differently stained images. While conventional staining methods involve time-consuming chemical processes, virtual…

We present a benchmark pilot study in which high-resolution Full-Field Optical Coherence Tomography (FF-OCT) is used to image human breast tissue and is evaluated to assess its ability to aid the pathologist's management of intra-operative…

Computational analysis of multiplexed immunofluorescence histology data is emerging as an important method for understanding the tumour micro-environment in cancer. This work presents HEMIT, a dataset designed for translating Hematoxylin…

图像与视频处理 · 电气工程与系统科学 2024-09-30 Chang Bian , Beth Philips , Tim Cootes , Martin Fergie

In recent years, the integration of advanced imaging techniques and deep learning methods has significantly advanced computer-aided diagnosis (CAD) systems for breast cancer detection and classification. Transformers, which have shown great…

计算机视觉与模式识别 · 计算机科学 2025-07-22 Mahtab Ranjbar , Mehdi Mohebbi , Mahdi Cherakhloo , Bijan Vosoughi. Vahdat

Dynamic full-field optical coherence microscopy (d-FF-OCM) is a label-free imaging technique that captures intrinsic subcellular motions to generate functional contrast. This dynamic approach yields images with fluorescence-like contrast,…

光学 · 物理学 2025-08-06 Erikas Tarvydas , Austeja Treciokaite , Egidijus Auksorius

Histological examination is crucial for cancer diagnosis, including hematoxylin and eosin (H&E) staining for mapping morphology and immunohistochemistry (IHC) staining for revealing chemical information. Recently developed two-color…

The field of histology relies heavily on antiquated tissue processing and staining techniques that limit the efficiency of pathologic diagnoses of cancer and other diseases. Current staining and advanced labeling methods are often…

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