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Monolayer semiconductors hold great potential for nanoscale electronics, optoelectronics, and photonics. Excitons dominate their optical properties. As their electric fields extend outside the monolayer, they are sensitive to their…

Super-resolution mapping of the 3D orientation of fluorophores reveals the alignment of biological structures where the fluorophores are tightly attached, and thus plays a vital role in studying the organization and dynamics of…

光学 · 物理学 2024-01-23 Xiaowei Liu , Mingwei Tang , Ning Zhou , Chenlei Pang , Zhong Wen , Xu Liu , Qing Yang

Extending super-resolution imaging techniques to objects hidden in strongly scattering media potentially revolutionize the technical analysis for much broader categories of samples, such as biological tissues. The main challenge is the…

This paper presents a comprehensive numerical analysis of super-resolution imaging using dielectric microparticles, employing the Finite-Difference Time-Domain (FDTD) method to elucidate the mechanisms that enable resolution enhancements…

光学 · 物理学 2024-09-18 Arlen Bekirov , Zengbo Wang , Boris Lukyanchuk

Super-resolution microscopy is crucial for imaging sub-wavelength biological structures. However, most techniques rely on nonlinear saturation or stochastic switching of emitters, limiting imaging speed and increasing phototoxicity. Here,…

Super-resolution localization microscopy is based on determining the positions of individual fluorescent markers in a sample. The major challenge in reaching an ever higher localization precision lies in the limited number of collected…

光学 · 物理学 2020-08-05 Daniel Böning , Franz-Ferdinand Wieser , Vahid Sandoghdar

We proposed a method to achieve superresolved optical imaging without beating the diffraction limit of light. This is achieved by magnifying the ideal optical image of the object through higher-order spatial frequency generation while…

光学 · 物理学 2016-08-08 Zhixiang Li , Jianji Liu , Guoquan Zhang

Fluorescence Correlation Spectroscopy (FCS) is widely used to detect and quantify diffusion processes at the molecular level. The molecules of which diffusion is studied are marked with fluorescent dyes. It is commonly maintained that this…

生物物理 · 物理学 2018-06-12 Mathias Lechelon , Marco Pettini

Single molecule localization microscopy (SMLM) techniques enable imaging biological samples well beyond the diffraction limit of light, but they vary significantly in their spatial and temporal resolutions. High-order statistical analysis…

生物物理 · 物理学 2016-03-15 Shachar Schidorsky , Xiyu Yi , Yair Razvag , Yonatan Golan , Shimon Weiss , Eilon Sherman

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

We provide a quantitative analysis of super-resolution imaging techniques which exploit temporal fluctuations of luminosity of the sources in order to beat the Rayleigh limit. We define an operationally justified resolution gain figure of…

量子物理 · 物理学 2021-06-08 Stanislaw Kurdzialek , Rafal Demkowicz-Dobrzanski

Existing super-resolution microscopy is often constrained by inherent trade-offs between resolution, acquisition speed, phototoxicity, and hardware complexity. Computational post-processing approaches offer a promising alternative, but they…

The resolution limits of classical spectroscopy can be surpassed by quantum-inspired methods leveraging the information contained in the phase of the complex electromagnetic field. Their counterpart in spatial imaging has been widely…

光学 · 物理学 2024-09-02 Michał Lipka , Michał Parniak

Spectral imaging is a fundamental diagnostic technique with widespread application. Conventional spectral imaging approaches have intrinsic limitations on spatial and spectral resolutions due to the physical components they rely on. To…

图像与视频处理 · 电气工程与系统科学 2021-06-07 Figen S. Oktem , Oğuzhan Fatih Kar , Can Deniz Bezek , Farzad Kamalabadi

Resolving sources beyond the diffraction limit is important in imaging, communications, and metrology. Current image-based methods of super-resolution require phase information (either of the source points or an added filter) and perfect…

光学 · 物理学 2025-12-16 S. A. Wadood , Shaurya Aarav , Kevin Liang , Jason W Fleischer

Hallucinating high frequency image details in single image super-resolution is a challenging task. Traditional super-resolution methods tend to produce oversmoothed output images due to the ambiguity in mapping between low and high…

计算机视觉与模式识别 · 计算机科学 2017-01-27 Libin Sun , James Hays

As proof-of-principle for generating superresolution structural information from DNA we applied a method of localization microscopy utilizing photoblinking comparing intercalating dye YOYO-1 against minor groove binding dye SYTO-13, using a…

生物大分子 · 定量生物学 2015-04-15 Helen Miller , Zhaokun Zhou , Adam J. M. Wollman , Mark C. Leake

Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) to segment the Fourier Plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional(3D)…

光学 · 物理学 2022-08-29 Haixin Huang , Haoyuan Qiu , Hanzhe Wu , Yihong Ji , Heng Li , Bin Yu , Danni Chen , Junle Qu

The ability to resolve detail in the object that is being imaged, named by resolution, is the core parameter of an imaging system. Super-resolution is a class of techniques that can enhance the resolution of an imaging system and even…

数据结构与算法 · 计算机科学 2022-10-13 Yaonan Jin , Daogao Liu , Zhao Song