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Organ transplantation serves as the primary therapeutic strategy for end-stage organ failures. However, allograft rejection is a common complication of organ transplantation. Histological assessment is essential for the timely detection and…

Histological analysis of tissue samples is one of the most widely used methods for disease diagnosis. After taking a sample from a patient, it goes through a lengthy and laborious preparation, which stains the tissue to visualize different…

计算机视觉与模式识别 · 计算机科学 2019-03-05 Yair Rivenson , Hongda Wang , Zhensong Wei , Yibo Zhang , Harun Gunaydin , Aydogan Ozcan

Histological staining is a vital step used to diagnose various diseases and has been used for more than a century to provide contrast to tissue sections, rendering the tissue constituents visible for microscopic analysis by medical experts.…

图像与视频处理 · 电气工程与系统科学 2020-05-08 Yijie Zhang , Kevin de Haan , Yair Rivenson , Jingxi Li , Apostolos Delis , Aydogan Ozcan

Histological staining is the gold standard for tissue examination in clinical pathology and life-science research, which visualizes the tissue and cellular structures using chromatic dyes or fluorescence labels to aid the microscopic…

医学物理 · 物理学 2023-03-07 Bijie Bai , Xilin Yang , Yuzhu Li , Yijie Zhang , Nir Pillar , Aydogan Ozcan

Deep learning-based virtual staining was developed to introduce image contrast to label-free tissue sections, digitally matching the histological staining, which is time-consuming, labor-intensive, and destructive to tissue. Standard…

图像与视频处理 · 电气工程与系统科学 2022-10-31 Yijie Zhang , Luzhe Huang , Tairan Liu , Keyi Cheng , Kevin de Haan , Yuzhu Li , Bijie Bai , Aydogan Ozcan

Modern histopathology relies on the microscopic examination of thin tissue sections stained with histochemical techniques, typically using brightfield or fluorescence microscopy. However, the staining of samples can permanently alter their…

Histopathological visualizations are a pillar of modern medicine and biological research. Surgical oncology relies exclusively on post-operative histology to determine definitive surgical success and guide adjuvant treatments. The current…

图像与视频处理 · 电气工程与系统科学 2022-04-04 Marian Boktor , Benjamin Ecclestone , Vlad Pekar , Deepak Dinakaran , John R. Mackey , Paul Fieguth , Parsin Haji Reza

Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the process is laborious and time-consuming,…

Virtual staining streamlines traditional staining procedures by digitally generating stained images from unstained or differently stained images. While conventional staining methods involve time-consuming chemical processes, virtual…

Pathological diagnosis relies on the visual inspection of histologically stained thin tissue specimens, where different types of stains are applied to bring contrast to and highlight various desired histological features. However, the…

医学物理 · 物理学 2022-08-18 Xilin Yang , Bijie Bai , Yijie Zhang , Yuzhu Li , Kevin de Haan , Tairan Liu , Aydogan Ozcan

In histopathology, tissue sections are typically stained using common H&E staining or special stains (MAS, PAS, PASM, etc.) to clearly visualize specific tissue structures. The rapid advancement of deep learning offers an effective solution…

图像与视频处理 · 电气工程与系统科学 2025-04-23 Zizhi Chen , Xinyu Zhang , Minghao Han , Yizhou Liu , Ziyun Qian , Weifeng Zhang , Xukun Zhang , Jingwei Wei , Lihua Zhang

Three-dimensional X-ray histology techniques offer a non-invasive alternative to conventional 2D histology, enabling volumetric imaging of biological tissues without the need for physical sectioning or chemical staining. However, the…

图像与视频处理 · 电气工程与系统科学 2025-09-12 Sarah C. Irvine , Christian Lucas , Diana Krüger , Bianca Guedert , Julian Moosmann , Berit Zeller-Plumhoff

The field of histology relies heavily on antiquated tissue processing and staining techniques that limit the efficiency of pathologic diagnoses of cancer and other diseases. Current staining and advanced labeling methods are often…

Virtual staining of histopathology images (e.g., H&E-IHC) is an emerging tool in digital pathology, enabling faster and cheaper workflows by synthesizing target stains from routinely acquired slides. Yet, the quality of virtual staining…

Hematoxylin and eosin (H&E) staining visualizes histology but lacks specificity for diagnostic markers. Immunohistochemistry (IHC) staining provides protein-targeted staining but is restricted by tissue availability and antibody…

图像与视频处理 · 电气工程与系统科学 2025-07-01 Anran Liu , Xiaofei Wang , Jing Cai , Chao Li

Multiplex imaging is revolutionizing pathology by enabling the simultaneous visualization of multiple biomarkers within tissue samples, providing molecular-level insights that traditional hematoxylin and eosin (H&E) staining cannot provide.…

图像与视频处理 · 电气工程与系统科学 2026-01-06 Hyun-Jic Oh , Junsik Kim , Zhiyi Shi , Yichen Wu , Yu-An Chen , Peter K Sorger , Hanspeter Pfister , Won-Ki Jeong

Virtual immunohistochemistry (IHC) staining from hematoxylin and eosin (H&E) images can accelerate diagnostics by providing preliminary molecular insight directly from routine sections, reducing the need for repeat sectioning when tissue is…

计算机视觉与模式识别 · 计算机科学 2026-03-16 Jillur Rahman Saurav , Thuong Le Hoai Pham , Pritam Mukherjee , Paul Yi , Brent A. Orr , Jacob M. Luber

In addition to evaluating tumor morphology using H&E staining, immunohistochemistry is used to assess the presence of specific proteins within the tissue. However, this is a costly and labor-intensive technique, for which virtual staining,…

计算机视觉与模式识别 · 计算机科学 2025-11-25 José Teixeira , Pascal Klöckner , Diana Montezuma , Melis Erdal Cesur , João Fraga , Hugo M. Horlings , Jaime S. Cardoso , Sara P. Oliveira

Pathology is practiced by visual inspection of histochemically stained slides. Most commonly, the hematoxylin and eosin (H&E) stain is used in the diagnostic workflow and it is the gold standard for cancer diagnosis. However, in many cases,…

Histopathological evaluation of tissue samples is a key practice in patient diagnosis and drug development, especially in oncology. Historically, Hematoxylin and Eosin (H&E) has been used by pathologists as a gold standard staining.…

计算机视觉与模式识别 · 计算机科学 2018-10-16 Amal Lahiani , Jacob Gildenblat , Irina Klaman , Shadi Albarqouni , Nassir Navab , Eldad Klaiman
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