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Knowledge of optical properties, such as the refractive index (RI), of biological tissues is important in optical imaging, as they influence the distribution and propagation of light in tissue. To accurately study the response of cancerous…

生物物理 · 物理学 2019-04-10 Neelam Hari , Priyanka Patel , Jacqueline Ross , Kevin Hicks , Frédérique Vanholsbeeck

Dynamic optical coherence tomography (DOCT) statistically analyzes fluctuations in time-sequential OCT signals, enabling label-free and three-dimensional visualization of intratissue and intracellular activities. Current DOCT methods, such…

Optical coherence tomography (OCT) is a label-free, non-invasive 3D imaging tool widely used in both biological research and clinical diagnosis. Current OCT modalities can only visualize specimen tomography without chemical information.…

Optical coherence tomography (OCT) is a powerful biomedical imaging technology that relies on the coherent detection of backscattered light to image tissue morphology in vivo. As a consequence, OCT is susceptible to coherent noise (speckle…

Optical coherence tomography (OCT) microscope (OCM) uses a high-numerical-aperture objective to achieve cellular-level lateral resolution. However, its practical imaging depth range is limited by the depth of focus (DOF). Although…

A prospective study was performed on neurosurgical samples from 18 patients to evaluate the use of Full-Field Optical Coherence Tomography (FF-OCT) in brain tumor diagnosis. FF-OCT captures en face slices of tissue samples at 1\mum…

Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds…

An organoid is a three-dimensional (3D) in vitro cell culture emulating human organs. We applied 3D dynamic optical coherence tomography (DOCT) to visualize the intratissue and intracellular activities of human induced pluripotent stem…

Objective: Ultrahigh-resolution optical coherence microscopy (OCM) has recently demonstrated its potential for accurate diagnosis of human cervical diseases. One major challenge for clinical adoption, however, is the steep learning curve…

计算机视觉与模式识别 · 计算机科学 2020-04-28 Yutao Ma , Tao Xu , Xiaolei Huang , Xiaofang Wang , Canyu Li , Jason Jerwick , Yuan Ning , Xianxu Zeng , Baojin Wang , Yihong Wang , Zhan Zhang , Xiaoan Zhang , Chao Zhou

We present a benchmark pilot study in which high-resolution Full-Field Optical Coherence Tomography (FF-OCT) is used to image human breast tissue and is evaluated to assess its ability to aid the pathologist's management of intra-operative…

The measurement of three-dimensional (3D) images and the analysis of subcellular organelles are crucial for the study of the pathophysiology of cells and tissues. Optical diffraction tomography (ODT) facilitates label-free and quantitative…

图像与视频处理 · 电气工程与系统科学 2019-10-08 Taean Chang , Seungwoo Shin , Moosung Lee , YongKeun Park

Current surgical procedures for spinal cord tumors lack in vivo high-resolution, high-speed multifunctional imaging systems, posing challenges for precise tumor resection and intraoperative decision-making. This study introduces the Fast…

Can a single label-free image reveal whether cancer cells were exposed to chemotherapy? We present an innovative methodology on the label-free and high-resolution imaging properties of phase holotomographic microscopy coupled with neural…

Dynamic full-field optical coherence microscopy (d-FF-OCM) is a label-free imaging technique that captures intrinsic subcellular motions to generate functional contrast. This dynamic approach yields images with fluorescence-like contrast,…

光学 · 物理学 2025-08-06 Erikas Tarvydas , Austeja Treciokaite , Egidijus Auksorius

Dynamic optical coherence tomography (DOCT) enables label-free functional imaging by capturing temporal OCT signal variations caused by intracellular and intratissue motions. However, the relationship between DOCT signals and the sample…

Optical diffraction tomography (ODT) is a three-dimensional (3D) label-free imaging technique. The 3D refractive index distribution of a sample can be reconstructed from multiple two-dimensional optical field images via ODT. Herein, we…

光学 · 物理学 2021-02-03 Chansuk Park , Kyeoreh Lee , Yoonseok Baek , Yongkeun Park

Label-free metabolic dynamics contrast is highly appealing but difficult to achieve in biomedical imaging. Interference offers a highly sensitive mechanism for capturing the metabolic dynamics of the subcellular scatterers. However,…

Label-free metabolic imaging of non-alcoholic fatty liver disease (NAFLD) mouse liver is demonstrated ex vivo by dynamic optical coherence tomography (OCT). The NAFLD mouse is a methionine choline-deficient (MCD)-diet model, and two mice…

Optical coherence tomography (OCT) is capable of non-destructively obtaining cross-sectional information of samples with micrometer spatial resolution, which plays an important role in ophthalmology and endovascular medicine. Measuring OCT…

医学物理 · 物理学 2023-11-30 Yang Jianlong , Zhang Haoran , Liu Chang , Gu Chengfu

Optical coherence tomography (OCT) is being increasingly adopted as a label-free and non-invasive technique for biomedical applications such as cancer and ocular disease diagnosis. Diagnostic information for these tissues is manifest in…

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