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Light-sheet fluorescence microscopy (LSFM) is used to capture volume images of biological specimens. It offers high contrast deep inside densely fluorescence labelled samples, fast acquisition speed and minimal harmful effects on the…

图像与视频处理 · 电气工程与系统科学 2024-04-05 Niklas Rottmayer , Claudia Redenbach , Florian Fahrbach

We report two techniques to mitigate stripe artifacts in light-sheet fluorescence imaging. The first uses an image processing algorithm called the multidirectional stripe remover (MDSR) method to filter stripes from an existing image. The…

仪器与探测器 · 物理学 2023-11-03 Seyyed Muhammad Salili , Matt Harrington , Douglas J. Durian

Microscopy such as Scanning Tunneling Microscopy (STM), Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM) are essential tools in material imaging at micro- and nanoscale resolutions to extract physical knowledge and…

Light sheet fluorescence microscopy (LSFM) has transformed the way we visualize biological tissues in three dimensions, offering high-resolution imaging while minimizing photo-induced damage to the samples. Recent breakthroughs in…

光学 · 物理学 2025-05-27 Alireza Tabatabaei Mashayekh , Jeremy Witzens

The non-uniform photoelectric response of infrared imaging systems results in fixed-pattern stripe noise being superimposed on infrared images, which severely reduces image quality. As the applications of degraded infrared images are…

图像与视频处理 · 电气工程与系统科学 2022-09-30 Zeshan Fayyaz , Daniel Platnick , Hannan Fayyaz , Nariman Farsad

Optical microscopy is an essential tool in biology and medicine. Imaging thin, yet non-flat objects in a single shot (without relying on more sophisticated sectioning setups) remains challenging as the shallow depth of field that comes with…

图像与视频处理 · 电气工程与系统科学 2020-10-14 Adrian Shajkofci , Michael Liebling

It was recently demonstrated [J. Electron. Imaging, 25(2), 2016] that one can perform fast non-local means (NLM) denoising of one-dimensional signals using a method called lifting. The cost of lifting is independent of the patch length,…

计算机视觉与模式识别 · 计算机科学 2018-03-14 Sanjay Ghosh , Kunal N. Chaudhury

We report on the recently emerging (Laser) Light Sheet based Fluorescence Microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects non-destructively in high-resolution through virtual…

仪器与探测器 · 物理学 2011-06-17 Jan A. N. Buytaert , Emilie Descamps , Dominique Adriaens , Joris J. J. Dirckx

Many microscopy applications are limited by the total amount of usable light and are consequently challenged by the resulting levels of noise in the acquired images. This problem is often addressed via (supervised) deep learning based…

图像与视频处理 · 电气工程与系统科学 2020-08-20 Anna S. Goncharova , Alf Honigmann , Florian Jug , Alexander Krull

Atomic Force Microscopy (AFM) enables high-resolution surface imaging at the nanoscale, yet the output is often degraded by artifacts introduced by environmental noise, scanning imperfections, and tip-sample interactions. To address this…

计算机视觉与模式识别 · 计算机科学 2026-02-05 Juntao Zhang , Angona Biswas , Jaydeep Rade , Charchit Shukla , Juan Ren , Anwesha Sarkar , Adarsh Krishnamurthy , Aditya Balu

Image deblurring aims to restore the detailed texture information or structures from blurry images, which has become an indispensable step in many computer vision tasks. Although various methods have been proposed to deal with the image…

计算机视觉与模式识别 · 计算机科学 2024-10-28 Yanni Zhang , Qiang Li , Miao Qi , Di Liu , Jun Kong , Jianzhong Wang

We study the problem of deconvolution for light-sheet microscopy, where the data is corrupted by spatially varying blur and a combination of Poisson and Gaussian noise. The spatial variation of the point spread function (PSF) of a…

A method for spatial deconvolution of spectra is presented. It follows the same fundamental principles as the ``MCS image deconvolution algorithm'' (Magain, Courbin, Sohy, 1998) and uses information contained in the spectrum of a reference…

天体物理学 · 物理学 2009-10-31 F. Courbin , P. Magain , M. Kirkove , S. Sohy

A lensfree spectral light-field fusion microscopy (LSLFM) system is presented for enabling contrast- and resolution-enhanced imaging of biological specimens. LSLFM consists of a pulsed multispectral lensfree microscope for capturing…

In multi-photon microscopy (MPM), a recent in-vivo fluorescence microscopy system, the task of image restoration can be decomposed into two interlinked inverse problems: firstly, the characterization of the Point Spread Function (PSF) and…

图像与视频处理 · 电气工程与系统科学 2024-11-08 Julien Ajdenbaum , Emilie Chouzenoux , Claire Lefort , Ségolène Martin , Jean-Christophe Pesquet

We propose a solution to the image deconvolution problem where the convolution kernel or point spread function (PSF) is assumed to be only partially known. Small perturbations generated from the model are exploited to produce a few…

数据分析、统计与概率 · 物理学 2015-06-04 Se Un Park , Nicolas Dobigeon , Alfred O. Hero

Light-field microscopy (LFM) enables rapid volumetric imaging through single-frame acquisition and fast 3D reconstruction algorithms. The high speed and low phototoxicity of LFM make it highly suitable for real-time 3D fluorescence imaging,…

光学 · 物理学 2025-02-24 Bohan Qu , Zhouyu Jin , You Zhou , Bo Xiong , Xun Cao

Machine learning (ML) methods are extraordinarily successful at denoising photographic images. The application of such denoising methods to scientific images is, however, often complicated by the difficulty in experimentally obtaining a…

Experimentally acquired microscopy images are unavoidably affected by the presence of noise and other unwanted signals, which degrade their quality and might hide relevant features. With the recent increase in image acquisition rate, modern…

图像与视频处理 · 电气工程与系统科学 2025-05-15 Marco Corrias , Giada Franceschi , Michele Riva , Alberto Tampieri , Karin Föttinger , Ulrike Diebold , Thomas Pock , Cesare Franchini

In this article, we present a denoising algorithm to improve the interpretation and quality of scanning tunneling microscopy (STM) images. Given the high level of self-similarity of STM images, we propose a denoising algorithm by…

计算机视觉与模式识别 · 计算机科学 2016-10-12 João P. Oliveira , Ana Bragança , José Bioucas-Dias , Mário Figueiredo , Luís Alcácer , Jorge Morgado , Quirina Ferreira
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