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We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…

光学 · 物理学 2019-04-03 Yoav N. Nygate , Gyanendra Singh , Itay Barnea , Natan T. Shaked

Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the…

Super-resolution fluorescence microscopy overcomes blurring arising from light diffraction, allowing the reconstruction of fine scale details in biological structures. Standard methods come at the expense of long acquisition time and/or…

最优化与控制 · 数学 2021-10-12 Bastien Laville , Laure Blanc-Féraud , Gilles Aubert

Beamforming in ultrasound imaging has significant impact on the quality of the final image, controlling its resolution and contrast. Despite its low spatial resolution and contrast, delay-and-sum is still extensively used nowadays in…

计算机视觉与模式识别 · 计算机科学 2016-05-20 Teodora Szasz , Adrian Basarab , Denis Kouamé

This work is concerned with optical imaging in strongly diffusive environments. We consider a typical setting in optical coherence tomography where a sample is probed by a collection of wavefields produced by a laser and propagating through…

光学 · 物理学 2024-12-10 Randy Bartels , Olivier Pinaud , Maxine Varughese

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

图像与视频处理 · 电气工程与系统科学 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Recent advances in optical technology have significantly enhanced the resolution of imaging of living cells, achieving nanometer-scale precision. However, the crowded three-dimensional environment within cells presents a challenge for…

光学 · 物理学 2024-11-20 Vahid Nasirimarekani , Zuzana Ditte , Eberhard Bodenschatz

Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light…

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Oblique plane microscopy is a method enabling light-sheet fluorescence imaging through a single microscope objective lens by focusing on a tilted plane within the sample. To focus the fluorescence emitted by the oblique plane on a camera,…

Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…

光学 · 物理学 2025-06-17 Ruiming Cao , Fanglin Linda Liu , Li-Hao Yeh , Laura Waller

Lensless imaging is a popular research field for the advantages of small size, wide field-of-view and low aberration in recent years. However, some traditional lensless imaging methods suffer from slow convergence, mechanical errors and…

Context. Narrow-band interference filters can be tuned toward shorter wavelengths by tilting them from the perpendicular to the optical axis. This can be used as a cheap alternative to real tunable filters, such as Fabry-P\'erot…

天体物理仪器与方法 · 物理学 2011-08-31 Mats G. Löfdahl , Vasco M. J. Henriques , Dan Kiselman

This study formally adapts the time-domain linear sampling method (TLSM) for ultrasonic imaging of stationary and evolving fractures in safety-critical components. The TLSM indicator is then applied to the laboratory test data of [22, 18]…

数值分析 · 数学 2022-09-16 Xiaoli Liu , Jian Song , Fatemeh Pourahmadian , Houssem Haddar

One of the main goals of modern observational cosmology is to map the large scale structure of the Universe. A potentially powerful approach for doing this would be to exploit three-dimensional spectral maps, i.e. the specific intensity of…

宇宙学与河外天体物理 · 物理学 2014-03-18 Roland de Putter , Gilbert P. Holder , Tzu-Ching Chang , Olivier Dore

Polarized light microscopy provides unique opportunities for analyzing the molecular order in man-made and natural materials, including biological structures inside living cells, tissues, and whole organisms. 20 years ago, the LC-PolScope…

光学 · 物理学 2015-06-16 Shalin B. Mehta , Michael Shribak , Rudolf Oldenbourg

Optical endomicroscopy (OEM) is an emerging technology platform with preclinical and clinical imaging applications. Pulmonary OEM via fibre bundles has the potential to provide in vivo, in situ molecular signatures of disease such as…

计算机视觉与模式识别 · 计算机科学 2018-08-29 Ahmed Karam Eldaly , Yoann Altmann , Antonios Perperidis , Nikola Krstajic , Tushar Choudhary , Kevin Dhaliwal , Stephen McLaughlin

Fourier Ptychographic Microscopy (FPM) is a computational imaging method that is able to super-resolve features beyond the diffraction-limit set by the objective lens of a traditional microscope. This is accomplished by using synthetic…

信号处理 · 电气工程与系统科学 2019-04-09 Michael Kellman , Emrah Bostan , Michael Chen , Laura Waller

Pink-beam Dark-Field X-ray Microscopy (pDFXM) is a powerful emerging technique for time-resolved studies of microstructure and strain evolution in bulk crystalline materials. In this work, we systematically assess the performance of pDFXM…

Single molecule localization microscopy (SMLM) techniques enable imaging biological samples well beyond the diffraction limit of light, but they vary significantly in their spatial and temporal resolutions. High-order statistical analysis…

生物物理 · 物理学 2016-03-15 Shachar Schidorsky , Xiyu Yi , Yair Razvag , Yonatan Golan , Shimon Weiss , Eilon Sherman