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相关论文: Ptychographic sensor for large-scale lensless micr…

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Ptychography is an enabling coherent diffraction imaging technique for both fundamental and applied sciences. Its applications in optical microscopy, however, fall short for its low imaging throughput and limited resolution. Here, we report…

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

图像与视频处理 · 电气工程与系统科学 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng

We report the implementation of a fully on-chip, lensless microscopy technique termed optofluidic ptychography. This imaging modality complements the miniaturization provided by microfluidics and allows the integration of ptychographic…

仪器与探测器 · 物理学 2021-12-21 Pengming Song , Chengfei Guo , Shaowei Jiang , Tianbo Wang , Patrick Hu , Derek Hu , Zibang Zhang , Bin Feng , Guoan Zheng

We report a new lensless microscopy configuration by integrating the concepts of transverse translational ptychography and defocus multi-height phase retrieval. In this approach, we place a tilted image sensor under the specimen for…

图像与视频处理 · 电气工程与系统科学 2021-11-03 Shaowei Jiang , Chengfei Guo , Patrick Hu , Derek Hu , Pengming Song , Tianbo Wang , Zichao Bian , Zibang Zhang , Guoan Zheng

Capturing biological specimens at large scales with sub-micron resolution is crucial for biomedical research, but conventional cameras often can't handle the pixel requirements. While most microscopes use motorized stages to move samples…

光学 · 物理学 2025-03-28 Charlie Kersuzan , Aymerick Bazin , Amaury Badon

We report a new coherent imaging technique, termed ptychographic structured modulation (PSM), for quantitative super-resolution microscopy. In this technique, we place a thin diffuser (i.e., a scattering lens) in between the sample and the…

图像与视频处理 · 电气工程与系统科学 2019-07-26 Pengming Song , Shaowei Jiang , He Zhang , Zichao Bian , Chengfei Guo , Kazunori Hoshino , Guoan Zheng

Bacterial heterogeneity is pivotal for adaptation to diverse environments, posing significant challenges in microbial diagnostics and therapeutic interventions. Recent advancements in high-resolution optical microscopy have revolutionized…

Conventional fiber-bundle-based endoscopes allow minimally invasive imaging through flexible multi-core fiber (MCF) bundles by placing a miniature lens at the distal tip and using each core as an imaging pixel. In recent years, lensless…

光学 · 物理学 2024-02-02 Gil Weinberg , Munkyu Kang , Wonjun Choi , Wonshik Choi , Ori Katz

Developing rapid methods for pathogen detection and growth monitoring at low cell and analyte concentrations is an important goal, which numerous technologies are working towards solving. Rapid biosensors have already made a dramatic impact…

Blu-ray drive is an engineering masterpiece that integrates disc rotation, pickup head translation, and three lasers in a compact and portable format. Here we integrate a blood-coated image sensor with a modified Blu-ray drive for…

仪器与探测器 · 物理学 2022-10-28 Shaowei Jiang , Chengfei Guo , Tianbo Wang , Jia Liu , Pengming Song , Terrance Zhang , Ruihai Wang , Bin Feng , Guoan Zheng

Imaging of biological cells and tissues often relies on fluorescent labels, which offer high contrast with molecular specificity. The use of exogenous labeling agents, however, may alter the normal physiology of the bio-specimens.…

仪器与探测器 · 物理学 2019-10-01 Zichao Bian , Shaowei Jiang , Pengming Song , He Zhang , Pouria Hoveida , Kazunori Hoshino , Guoan Zheng

Achieving gigapixel space-bandwidth products (SBP) at video rates represents a fundamental challenge in imaging science. Here we demonstrate video-rate ptychography that overcomes this barrier by exploiting spatiotemporal correlations…

Bacteria sense a diverse range of environmental analytes with high sensitivity and temporal resolution. Engineering and synthetic biology approaches enabled harnessing this capability through development of whole-cell biosensors that…

Ptychography is a lensless, computational imaging method that utilises diffraction patterns to determine the amplitude and phase of an object. In transmission ptychography, the diffraction patterns are recorded by a detector positioned…

图像与视频处理 · 电气工程与系统科学 2023-01-04 Kira A. M. Maathuis , Jacob Seifert , Allard P. Mosk

Lensless on-chip microscopy has shown great potential for biomedical imaging due to its large-area and high-throughput imaging capabilities. By combining the pixel super-resolution (PSR) technique, it can improve the resolution beyond the…

图像与视频处理 · 电气工程与系统科学 2024-09-06 Xuyang Chang , Shaowei Jiang , Yongcun Hu , Liheng Bian

We present a computational live bacteria detection system that periodically captures coherent microscopy images of bacterial growth inside a 60 mm diameter agar-plate and analyzes these time-lapsed holograms using deep neural networks for…

We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at 5 wavelengths simultaneously. A prism is added at…

Live-cell microscopy allows to go beyond measuring average features of cellular populations to observe, quantify and explain biological heterogeneity. Deep Learning-based instance segmentation and cell tracking form the gold standard…

定量方法 · 定量生物学 2024-08-09 Richard D. Paul , Johannes Seiffarth , Hanno Scharr , Katharina Nöh

Ptychography is an emerging imaging technique that is able to provide wavelength-limited spatial resolution from specimen with extended lateral dimensions. As a scanning microscopy method, a typical two-dimensional image requires a number…

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in…

定量方法 · 定量生物学 2022-06-07 Santosh Pandey , Yunsoo Park , Ankita Ankita , Gregory J. Phillips
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