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We investigate the dynamics of an active gel of bundled microtubules that is driven by clusters of kinesin molecular motors. Upon the addition of ATP, the coordinated action of thousands of molecular motors drives the gel to a highly…

软凝聚态物质 · 物理学 2015-06-23 Gil Henkin , Stephen J. DeCamp , Daniel TN Chen , Tim Sanchez , Zvonimir Dogic

Cytoskeletal networks are foundational examples of active matter and central to self-organized structures in the cell. In vivo, these networks are active and heavily crosslinked. Relating their large-scale dynamics to properties of their…

The cytoskeleton relies on diverse populations of motors, filaments, and binding proteins acting in concert to enable non-equilibrium processes ranging from mitosis to chemotaxis. Its versatile reconfigurability, programmed by interactions…

The cytoskeleton is regulated by a plethora of enzymes that influence the stability and dynamics of cytoskeletal filaments. Molecular motors of the kinesin-8 protein family depolymerise microtubules in a length-dependent manner, and…

亚细胞过程 · 定量生物学 2014-10-29 Louis Reese , Anna Melbinger , Erwin Frey

In nature interactions between biopolymers and motor proteins give rise to biologically essential emergent behaviours. Besides cytoskeleton mechanics, active nematics arise from such interactions. Here we present a study on 3D active…

In the presence of ATP, molecular motors generate active force dipoles that drive suspensions of protein filaments far from thermodynamic equilibrium, leading to exotic dynamics and pattern formation. Microscopic modelling can help to…

生物物理 · 物理学 2015-06-17 D. A. Head , W. J. Briels , G. Gompper

We have developed several distinct model systems of microtubule-based 3D active isotropic fluids and have compared their dynamical and structural properties. The non-equilibrium dynamics of these fluids is powered by three different types…

Motor-driven cytoskeletal remodeling in cellular systems can often be accompanied by a diffusive-like effect at local scales, but distinguishing the contributions of the ordering process, such as active contraction of a network, from this…

Motor-proteins are responsible for transport inside cells. Harnessing their activity is key towards developing new nano-technologies, or functional biomaterials. Cytoskeleton-like networks, recently tailored in vitro, result from the…

软凝聚态物质 · 物理学 2016-06-21 Pau Guillamat , Jordi Ignés-Mullol , Francesc Sagués

The cytoskeleton is an active composite of filamentous proteins that dictates diverse mechanical properties and processes in eukaryotic cells by generating forces and autonomously restructuring itself. Enzymatic motors that act on the…

Active fluids have applications in micromixing, but little is known about the mixing kinematics of systems with spatiotemporally-varying activity. To investigate, UV-activated caged ATP was used to activate controlled regions of…

软凝聚态物质 · 物理学 2022-11-07 Teagan E Bate , Megan E Varney , Ezra H Taylor , Joshua H Dickie , Chih-Che Chueh , Michael M Norton , Kun-Ta Wu

Within living cells, the transport of cargo is accomplished by groups of molecular motors. Such collective transport could utilize mechanisms which emerge from inter-motor interactions in ways that are yet to be fully understood. Here we…

生物大分子 · 定量生物学 2016-10-04 David Ando , Michelle K. Mattson , Jing Xu , Ajay Gopinathan

We study the dynamics and phase behaviour of a dry suspension of microtubules and molecular motors. We obtain a set of continuum equations by rigorously coarse graining a microscopic model where motor-induced interactions lead to parallel…

软凝聚态物质 · 物理学 2018-06-27 Ivan Maryshev , Andrew B. Goryachev , Davide Marenduzzo , Alexander Morozov

Biomolecular motor proteins that generate forces by consuming chemical energy obtained from ATP hydrolysis are pivotal for organizing broad cytoskeletal structures in living cells. The control of such cytoskeletal structures benefits…

软凝聚态物质 · 物理学 2021-12-30 Shunya Araki , Kazusa Beppu , Arif Md. Rashedul Kabir , Akira Kakugo , Yusuke T. Maeda

Active stresses, which are collectively generated by the motion of energy-consuming rod-like constituents, generate chaotic autonomous flows. Controlling active stresses in space and time is an essential prerequisite for controlling the…

软凝聚态物质 · 物理学 2022-09-15 Linnea M. Lemma , Minu Varghese , Tyler D. Ross , Matt Thomson , Aparana Baskaran , Zvonimir Dogic

Living cells dynamically modulate the local morphologies of their actin cytoskeletons to perform biological functions, including force transduction, intracellular transport, and cell division. A major challenge is to understand how diverse…

生物物理 · 物理学 2018-12-07 Simon L. Freedman , Glen M. Hocky , Shiladitya Banerjee , Aaron R. Dinner

With exquisite precision and reproducibility, cells orchestrate the cooperative action of thousands of nanometer-sized molecular motors to carry out mechanical tasks at much larger length scales, such as cell motility, division and…

软凝聚态物质 · 物理学 2013-01-08 Tim Sanchez , Daniel T. N. Chen , Stephen J. DeCamp , Michael Heymann , Zvonimir Dogic

Myosin motor proteins drive vigorous steady-state fluctuations in the actin cytoskeleton of cells. Endogenous embedded semiflexible filaments such as microtubules, or added filaments such as single-walled carbon nanotubes are used as novel…

生物物理 · 物理学 2016-06-21 J. Gladrow , N. Fakhri , F. C. MacKintosh , C. F. Schmidt , C. P. Broedersz

The cytoskeleton is a model active matter system that controls diverse cellular processes from division to motility. While both active actomyosin dynamics and actin-microtubule interactions are key to the cytoskeleton's versatility and…

The cellular cytoskeleton is an active material, driven out of equilibrium by molecular motor proteins. It is not understood how the collective behaviors of cytoskeletal networks emerge from the properties of the network's constituent motor…

亚细胞过程 · 定量生物学 2017-12-19 Peter J. Foster , Wen Yan , Sebastian Fürthauer , Michael J. Shelley , Daniel J. Needleman
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