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Laser Doppler holography (LDH) is a full-field imaging technique that was recently used in the human eye to reveal blood flow contrasts in the retinal and choroidal vasculature non-invasively, and with high temporal resolution. We here…

医学物理 · 物理学 2020-04-01 L. Puyo , M. Paques , M. Fink , J-A Sahel , M. Atlan

The choroid is a highly vascularized tissues supplying the retinal pigment epithelium and photoreceptors. Its implication in retinal diseases is gaining increasing interest. However, investigating the anatomy and flow of the choroid remains…

医学物理 · 物理学 2021-06-02 Léo Puyo , Michel Paques , Mathias Fink , José-Alain Sahel , Michael Atlan

A comprehensive assessment of retinal health demands reliable and precise methods to measure localized blood perfusion. Despite considerable advancements in imaging techniques, such as indocyanine green and fluorescein angiography, along…

医学物理 · 物理学 2024-03-15 Michael Atlan

Laser Doppler holography is a planar blood flow imaging technique recently introduced in ophthalmology to image human retinal and choroidal blood flow non-invasively. Here we present a digital method based on the Doppler spectrum asymmetry…

医学物理 · 物理学 2021-08-10 Léo Puyo , Michel Paques , Michael Atlan

This study presents a novel quantitative estimation method for total retinal arterial blood flow utilizing real-time Doppler holography at an unprecedented frame rate of 33,000 frames per second. This technique, leveraging high-speed…

图像与视频处理 · 电气工程与系统科学 2024-09-27 Yann Fischer , Zacharie Auray , Olivier Martinache , Marius Dubosc , Noé Topéza , Chloé Magnier , Maxime Boy-Arnould , Michael Atlan

Laser Doppler holography (LDH) is a full-field blood flow imaging technique able to reveal human retinal and choroidal blood flow with high temporal resolution. We here report on using LDH in the anterior segment of the eye without making…

The eye offers a unique opportunity for non-invasive exploration of cardiovascular diseases. Optical angiography in the retina requires sensitive measurements, which hinders conventional full-field laser Doppler imaging schemes. To overcome…

医学物理 · 物理学 2018-09-18 L. Puyo , M. Paques , M. Fink , J. -A. Sahel , M. Atlan

Doppler holography is an emerging retinal imaging technique that captures the dynamic behavior of blood flow with high temporal resolution, enabling quantitative assessment of retinal hemodynamics. This requires accurate segmentation of…

计算机视觉与模式识别 · 计算机科学 2026-02-20 Marius Dubosc , Yann Fischer , Zacharie Auray , Nicolas Boutry , Edwin Carlinet , Michael Atlan , Thierry Geraud

We report on local superficial blood flow monitoring in biological tissue from laser Doppler holographic imaging. In time averaging recording conditions, holography acts as a narrowband bandpass filter, which, combined with a frequency…

High-speed digital holography reveals local blood flow contrasts in the eye fundus through deterministic signal analysis, leveraging a forward scattering model of dynamically diffused light. This approach enables the estimation of absolute…

仪器与探测器 · 物理学 2025-05-14 Zacharie Auray , Yann Fischer , Olivier Martinache , Michael Atlan

Laser Doppler holography (LDH) is an interferometric blood flow imaging technique based on full-field measurements of the Doppler spectrum. LDH has so far been demonstrated in the retina with ultrafast cameras, typically at 75 kHz. We show…

图像与视频处理 · 电气工程与系统科学 2020-08-26 Leo Puyo , Michel Paques , Michael Atlan

Full-field swept-source optical coherence tomography (FF-SS-OCT) and laser Doppler holography (LDH) are two holographic imaging techniques presenting unique capabilities for ophthalmology. We report on interlaced FF-SS-OCT and LDH imaging…

医学物理 · 物理学 2022-03-01 Léo Puyo , Hendrik Spahr , Clara Pfäffle , Gereon Hüttmann , Dierck Hillmann

We performed non-invasive video imaging of retinal blood flow in a pigmented rat by holographic interferometry of near-infrared laser light backscattered by retinal tissue, beating against an off-axis reference beam sampled at a frame rate…

Laser Doppler holography (LDH) is a full-field interferometric imaging technique recently applied in ophthalmology to measure blood flow, a parameter of high clinical interest. From the temporal fluctuations of digital holograms acquired at…

图像与视频处理 · 电气工程与系统科学 2020-05-13 Leo Puyo , Michel Paques , Michael Atlan

We report on using a laser Doppler processing of Fourier-domain optical coherence tomography (OCT) data for the assessment of pulsatile blood flow in the choriocapillaris. Signal fluctuations in B-scans recorded at 2 kHz were analyzed by…

We report on wide-field imaging of pulsatile motion induced by blood flow using heterodyne holographic interferometry on the thumb of a healthy volunteer, in real-time. Optical Doppler images were measured with green laser light by a…

光学 · 物理学 2015-04-01 Jeffrey Bencteux , Pierre Pagnoux , Thomas Kostas , Sam Bayat , Michael Atlan

We have extended Laser Doppler holographic microscopy to transmission geometry. The technique is validated with living fish embryos imaged by a modified upright bio-microcope. By varying the frequency of the holographic reference beam, and…

光学 · 物理学 2014-05-01 Nicolas Verrier , Daniel Alexandre , Michel Gross

We report a pilot study with a wide-field laser Doppler detection scheme used to perform laser Doppler anemometry and imaging of particle seeded microflow. The optical field carrying the local scatterers (particles) dynamic state, as a…

光学 · 物理学 2013-12-24 Michel Gross , Michael Atlan , Jacques Leng

We report laser Doppler ophthalmoscopic fundus imaging in the rat eye with near-IR heterodyne holography. Sequential sampling of the beat of the reflected radiation against a frequency-shifted optical local oscillator is made onto an array…

The variation of blood flow characteristics caused by the probe pressure during noninvasive studies is of particular interest within the context of fundamental and applied research. It has been shown previously that the weak local pressure…

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