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Single cell segmentation is critical and challenging in live cell imaging data analysis. Traditional image processing methods and tools require time-consuming and labor-intensive efforts of manually fine-tuning parameters. Slight variations…

Cell instance segmentation in fluorescence microscopy images is becoming essential for cancer dynamics and prognosis. Data extracted from cancer dynamics allows to understand and accurately model different metabolic processes such as…

This paper addresses the problem of quantifying biomarkers in multi-stained tissues, based on color and spatial information. A deep learning based method that can automatically localize and quantify the cells expressing biomarker(s) in a…

组织与器官 · 定量生物学 2017-01-02 Fahime Sheikhzadeh , Martial Guillaud , Rabab K. Ward

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

计算机视觉与模式识别 · 计算机科学 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

While most previous automation-assisted reading methods can improve efficiency, their performance often relies on the success of accurate cell segmentation and hand-craft feature extraction. This paper presents an efficient and totally…

计算机视觉与模式识别 · 计算机科学 2019-12-17 Yao Xiang , Wanxin Sun , Changli Pan , Meng Yan , Zhihua Yin , Yixiong Liang

Background: Image analysis applications in digital pathology include various methods for segmenting regions of interest. Their identification is one of the most complex steps, and therefore of great interest for the study of robust methods…

计算机视觉与模式识别 · 计算机科学 2022-12-29 Giuseppe Giacopelli , Michele Migliore , Domenico Tegolo

Advances in fluorescence microscopy enable acquisition of 3D image volumes with better image quality and deeper penetration into tissue. Segmentation is a required step to characterize and analyze biological structures in the images and…

计算机视觉与模式识别 · 计算机科学 2019-04-16 Chichen Fu , Soonam Lee , David Joon Ho , Shuo Han , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Accurate detection and counting of virus patches in focus-forming unit (FFU) images, also known as foci images, are important for quantifying viral infection and analyzing cellular structures. This task is challenging because biomedical…

计算机视觉与模式识别 · 计算机科学 2026-05-22 Amrita Singh , Snehasis Mukherjee

X-Ray image enhancement, along with many other medical image processing applications, requires the segmentation of images into bone, soft tissue, and open beam regions. We apply a machine learning approach to this problem, presenting an…

计算机视觉与模式识别 · 计算机科学 2019-04-23 Joseph Bullock , Carolina Cuesta-Lazaro , Arnau Quera-Bofarull

Image segmentation in total knee arthroplasty is crucial for precise preoperative planning and accurate implant positioning, leading to improved surgical outcomes and patient satisfaction. The biggest challenges of image segmentation in…

图像与视频处理 · 电气工程与系统科学 2024-05-28 Viet Dung Nguyen , Michael T. LaCour , Richard D. Komistek

Cell detection and segmentation is fundamental for all downstream analysis of digital pathology images. However, obtaining the pixel-level ground truth for single cell segmentation is extremely labor intensive. To overcome this challenge,…

计算机视觉与模式识别 · 计算机科学 2019-10-29 Alireza Chamanzar , Yao Nie

Accurate localization of proteins from fluorescence microscopy images is challenging due to the inter-class similarities and intra-class disparities introducing grave concerns in addressing multi-class classification problems. Conventional…

计算机视觉与模式识别 · 计算机科学 2021-10-11 Muhammad Tahir , Saeed Anwar , Ajmal Mian , Abdul Wahab Muzaffar

In this paper, we introduce a conceptually simple network for generating discriminative tissue-level segmentation masks for the purpose of breast cancer diagnosis. Our method efficiently segments different types of tissues in breast biopsy…

计算机视觉与模式识别 · 计算机科学 2018-06-06 Sachin Mehta , Ezgi Mercan , Jamen Bartlett , Donald Weave , Joann G. Elmore , Linda Shapiro

Digital pathology has recently been revolutionized by advancements in artificial intelligence, deep learning, and high-performance computing. With its advanced tools, digital pathology can help improve and speed up the diagnostic process,…

图像与视频处理 · 电气工程与系统科学 2024-03-28 Mohamed Elmanna , Ahmed Elsafty , Yomna Ahmed , Muhammad Rushdi , Ahmed Morsy

Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…

定量方法 · 定量生物学 2025-08-26 Surajit Das , Gourav Roy , Pavel Zun

We present a weak to strong generalization methodology for fully automated training of a multi-head extension of the Mask-RCNN method with efficient channel attention for reliable segmentation of overlapping cell nuclei in multiplex cyclic…

计算机视觉与模式识别 · 计算机科学 2025-12-15 Lin Bai , Xiaoyang Li , Liqiang Huang , Quynh Nguyen , Hien Van Nguyen , Saurabh Prasad , Dragan Maric , John Redell , Pramod Dash , Badrinath Roysam

Instance segmentation and classification of nuclei is an important task in computational pathology. We show that StarDist, a deep learning nuclei segmentation method originally developed for fluorescence microscopy, can be extended and…

计算机视觉与模式识别 · 计算机科学 2022-08-22 Martin Weigert , Uwe Schmidt

This paper introduces a comprehensive approach for segmenting regions of interest (ROI) in diverse medical imaging datasets, encompassing ultrasound, CT scans, and X-ray images. The proposed method harnesses the capabilities of the YOLOv8…

计算机视觉与模式识别 · 计算机科学 2023-10-23 Sumit Pandey , Kuan-Fu Chen , Erik B. Dam

We propose a 3D convolutional neural network to simultaneously segment and detect cell nuclei in confocal microscopy images. Mirroring the co-dependency of these tasks, our proposed model consists of two serial components: the first part…

图像与视频处理 · 电气工程与系统科学 2018-09-07 Sundaresh Ram , Vicky T. Nguyen , Kirsten H. Limesand , Mert R. Sabuncu

Cell segmentation is essential in biomedical research for analyzing cellular morphology and behavior. Deep learning methods, particularly convolutional neural networks (CNNs), have revolutionized cell segmentation by extracting intricate…

图像与视频处理 · 电气工程与系统科学 2024-08-27 Boyuan Peng , Jiaju Chen , P. Bilha Githinji , Ijaz Gul , Qihui Ye , Minjiang Chen , Peiwu Qin , Xingru Huang , Chenggang Yan , Dongmei Yu , Jiansong Ji , Zhenglin Chen