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The technique of remote refocusing is used in optical microscopy to provide rapid axial scanning without mechanically perturbing the sample and in techniques such as oblique plane microscopy that build on remote refocusing to image a tilted…

光学 · 物理学 2023-09-27 Wenzhi Hong , Hugh Sparks , Chris Dunsby

Wide field-of-view (FOV) cameras, which capture a larger scene area than narrow FOV cameras, are used in many applications including 3D reconstruction, autonomous driving, and video surveillance. However, wide-angle images contain…

计算机视觉与模式识别 · 计算机科学 2021-12-02 Jinlong Fan , Jing Zhang , Stephen J. Maybank , Dacheng Tao

High-throughput 2D and 3D scanning electron microscopy, which relies on automation and dependable control algorithms, requires high image quality with minimal human intervention. Classical focus and astigmatism correction algorithms attempt…

仪器与探测器 · 物理学 2023-05-10 Philipp Johannes Schubert , Rangoli Saxena , Joergen Kornfeld

Most algorithms in 3D computer vision rely on the pinhole camera model because of its simplicity, whereas virtually all imaging devices introduce certain amount of nonlinear distortion, where the radial distortion is the most severe part.…

计算机视觉与模式识别 · 计算机科学 2007-05-23 Lili Ma , YangQuan Chen , Kevin L. Moore

Off-axis parabolic mirrors (OAPs) are occasionally desirable for specialized applications, but are known to introduce field-dependent astigmatic aberrations. In an experiment where optical tweezers are formed by OAPs, another OAP is added…

光学 · 物理学 2025-11-20 Meimei Liu , Yuqi Zhu , Giorgio Gratta

Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function because of its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by…

生物物理 · 物理学 2020-01-08 Courtney Johnson , Jack Exell , Jonathon Kuo , Kevin Welsher

Nonlinear lens distortion rectification is a common first step in image processing applications where the assumption of a linear camera model is essential. For rectifying the lens distortion, forward distortion model needs to be known.…

计算机视觉与模式识别 · 计算机科学 2017-07-05 Burak Benligiray , Cihan Topal

Acousto-optic deflectors (AODs) are widely used across physics, microscopy, neuroscience, and laser engineering, providing fast, precise, and non-mechanical control of light. While conventional AODs naturally support multiplexing in one and…

光学 · 物理学 2025-10-10 Lewis R. B. Picard , Manuel Endres

Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…

仪器与探测器 · 物理学 2019-12-03 Gaelle Recher , Pierre Nassoy , Amaury Badon

We propose a novel method for non-rigid 3-D motion correction of orthogonally raster-scanned optical coherence tomography angiography volumes. This is the first approach that aligns predominantly axial structural features like retinal…

A simple method of constructing the 3D surface of non-transparent micro-objects by extending the depth-of-field on the whole attainable surface is presented. The series of images of a sample are recorded by the sequential movement of the…

仪器与探测器 · 物理学 2009-04-15 V. V. Berejnov

Volumetric optical microscopy using non-diffracting beams enables rapid imaging of 3D volumes by projecting them axially to 2D images but lacks crucial depth information. Addressing this, we introduce MicroDiffusion, a pioneering tool…

图像与视频处理 · 电气工程与系统科学 2024-03-19 Mude Hui , Zihao Wei , Hongru Zhu , Fei Xia , Yuyin Zhou

Autofocus (AF) methods are extensively used in biomicroscopy, for example to acquire timelapses, where the imaged objects tend to drift out of focus. AD algorithms determine an optimal distance by which to move the sample back into the…

图像与视频处理 · 电气工程与系统科学 2021-01-14 Adrian Shajkofci , Michael Liebling

We present a deep learning driven computational approach to overcome the limitations of self-interference digital holography that imposed by inferior axial imaging performances. We demonstrate a 3D deep neural network model can…

This paper presents a blind detection and compensation technique for camera lens geometric distortions. The lens distortion introduces higher-order correlations in the frequency domain and in turn it can be detected using higher-order…

计算机视觉与模式识别 · 计算机科学 2007-05-23 Lili Ma , YangQuan Chen , Kevin L. Moore

Three-photon (3-P) fluorescence microscopy enables deep in vivo imaging with subcellular resolution, but its performance is fundamentally constrained by the maximum permissible laser power required to avoid tissue heating and photodamage.…

光学 · 物理学 2026-03-04 Qi Hu , Jingyu Wang , Huriye Atilgan , Armin Lak , Martin J. Booth

Motion during image acquisition can cause image degradation in all medical imaging modalities. This is particularly relevant in 2-D ultrasound imaging, since out-of-plane motion can only be compensated for movements smaller than elevational…

医学物理 · 物理学 2019-02-07 S. Harput , K. Christensen-Jeffries , J. Brown , J. Zhu , G. Zhang , R. J. Eckersley , C. Dunsby , M-X. Tang

We demonstrate a new versatile method for 3D super-resolution microscopy by using a deformable mirror to shape the point spread function of our microscope in a continuous and controllable way. We apply this for 3D STORM imaging of…

光学 · 物理学 2014-01-07 Nicolas Piro , Thomas Pengo , Nicolas Olivier , Suliana Manley

Multifocal plane microscopy (MUM) allows three dimensional objects to be imaged in a single camera frame. Our approach uses dual orthogonal diffraction phase gratings with a quadratic distortion of the lines to apply defocus to the first…

光学 · 物理学 2020-03-13 Benjamin Gore , Noah Schwartz , Paul Dalgarno

Accurate camera calibration is a fundamental task for 3D perception, especially when dealing with real-world, in-the-wild environments where complex optical distortions are common. Existing methods often rely on pre-rectified images or…

计算机视觉与模式识别 · 计算机科学 2025-03-28 Liuyue Xie , Jiancong Guo , Ozan Cakmakci , Andre Araujo , Laszlo A. Jeni , Zhiheng Jia
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